Bark beetle-associated genomovirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus denbre1

Average proteome isoelectric point is 7.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A344A3P5|A0A344A3P5_9VIRU Replication-associated protein OS=Bark beetle-associated genomovirus 1 OX=2230896 PE=3 SV=1
MM1 pKa = 7.82PFACNARR8 pKa = 11.84YY9 pKa = 8.96FLVTYY14 pKa = 8.7GHH16 pKa = 6.38VEE18 pKa = 4.01SLDD21 pKa = 3.42PFALVDD27 pKa = 3.58HH28 pKa = 6.89FGKK31 pKa = 10.67LGAEE35 pKa = 4.21IIVSLEE41 pKa = 3.82QYY43 pKa = 10.33NATLGVHH50 pKa = 5.51FHH52 pKa = 6.04VFADD56 pKa = 4.64FGRR59 pKa = 11.84KK60 pKa = 8.16FRR62 pKa = 11.84SRR64 pKa = 11.84RR65 pKa = 11.84TDD67 pKa = 2.73IFDD70 pKa = 3.67VEE72 pKa = 4.64GFHH75 pKa = 7.31PNISPSRR82 pKa = 11.84GTPEE86 pKa = 3.98AGYY89 pKa = 10.62DD90 pKa = 3.63YY91 pKa = 10.68AIKK94 pKa = 10.92DD95 pKa = 3.59GDD97 pKa = 4.02VVAGGLARR105 pKa = 11.84PSGVVASGRR114 pKa = 11.84QAKK117 pKa = 9.23WNTILDD123 pKa = 3.75AEE125 pKa = 4.57TRR127 pKa = 11.84DD128 pKa = 3.91EE129 pKa = 4.71FCALCEE135 pKa = 3.95EE136 pKa = 5.09LDD138 pKa = 3.97RR139 pKa = 11.84EE140 pKa = 4.14RR141 pKa = 11.84LVCSFGQIQKK151 pKa = 9.89FADD154 pKa = 2.8WRR156 pKa = 11.84FAVEE160 pKa = 3.92PEE162 pKa = 4.75PYY164 pKa = 10.07VSPDD168 pKa = 3.13GVFDD172 pKa = 4.04LADD175 pKa = 4.02YY176 pKa = 11.38GDD178 pKa = 3.67LGYY181 pKa = 10.33WRR183 pKa = 11.84DD184 pKa = 3.43NFLFADD190 pKa = 3.56TTGRR194 pKa = 11.84SKK196 pKa = 11.16SLILWGPSRR205 pKa = 11.84MGKK208 pKa = 6.86TVWARR213 pKa = 11.84SLGRR217 pKa = 11.84HH218 pKa = 6.15LYY220 pKa = 10.37FGGIFSARR228 pKa = 11.84NIGDD232 pKa = 3.2DD233 pKa = 3.86RR234 pKa = 11.84IRR236 pKa = 11.84FAVFDD241 pKa = 5.4DD242 pKa = 3.3IAGGIKK248 pKa = 10.03FFPRR252 pKa = 11.84FKK254 pKa = 10.79DD255 pKa = 3.12WLGCQMEE262 pKa = 4.42FMVKK266 pKa = 9.55EE267 pKa = 4.57LYY269 pKa = 10.07RR270 pKa = 11.84DD271 pKa = 3.33PHH273 pKa = 6.29LFRR276 pKa = 11.84WGRR279 pKa = 11.84PAIWIANSDD288 pKa = 3.56PRR290 pKa = 11.84HH291 pKa = 6.58DD292 pKa = 3.89MTHH295 pKa = 7.25DD296 pKa = 4.74DD297 pKa = 4.35IVWLEE302 pKa = 3.92ANCIFVEE309 pKa = 4.22ISSPIFRR316 pKa = 11.84ANTT319 pKa = 3.33

Molecular weight: 36.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A344A3P5|A0A344A3P5_9VIRU Replication-associated protein OS=Bark beetle-associated genomovirus 1 OX=2230896 PE=3 SV=1
MM1 pKa = 7.95AYY3 pKa = 10.09RR4 pKa = 11.84RR5 pKa = 11.84GGTARR10 pKa = 11.84KK11 pKa = 9.52SYY13 pKa = 10.48KK14 pKa = 9.14ATAKK18 pKa = 9.79RR19 pKa = 11.84RR20 pKa = 11.84YY21 pKa = 9.36AGRR24 pKa = 11.84SKK26 pKa = 10.51FRR28 pKa = 11.84RR29 pKa = 11.84SYY31 pKa = 10.51AKK33 pKa = 9.91KK34 pKa = 7.74RR35 pKa = 11.84TYY37 pKa = 10.27RR38 pKa = 11.84KK39 pKa = 9.67RR40 pKa = 11.84LISIKK45 pKa = 10.51AVLNVTSRR53 pKa = 11.84KK54 pKa = 9.23KK55 pKa = 10.47RR56 pKa = 11.84NGMLSWSNTNPGTGASQTIAAGGAVVAGNSVGFFLFSPTCMNLNQGSSNPTYY108 pKa = 10.4FINSAEE114 pKa = 4.24RR115 pKa = 11.84TSTTCYY121 pKa = 9.46MRR123 pKa = 11.84GFSEE127 pKa = 4.31TLRR130 pKa = 11.84IQTNSHH136 pKa = 5.34VPWFHH141 pKa = 7.26RR142 pKa = 11.84RR143 pKa = 11.84ICFCFRR149 pKa = 11.84GATPFASPNSSDD161 pKa = 3.36TPTQNMSPYY170 pKa = 9.75VDD172 pKa = 3.45TSNGMEE178 pKa = 4.14RR179 pKa = 11.84LWLNQQVNAMGQSVQAIWGVLFKK202 pKa = 11.39GMINQDD208 pKa = 2.97WNDD211 pKa = 3.5VVIAPVDD218 pKa = 3.6TTRR221 pKa = 11.84VDD223 pKa = 3.78LKK225 pKa = 10.96FDD227 pKa = 3.58KK228 pKa = 10.49TWLIKK233 pKa = 10.62SGNEE237 pKa = 3.51SGTIVQRR244 pKa = 11.84KK245 pKa = 7.82LWHH248 pKa = 6.5PMNKK252 pKa = 9.58NLVYY256 pKa = 10.84DD257 pKa = 4.31DD258 pKa = 5.62DD259 pKa = 4.21EE260 pKa = 4.93TGEE263 pKa = 4.4SMASQYY269 pKa = 11.39YY270 pKa = 10.32SVDD273 pKa = 3.43SKK275 pKa = 11.56QGMGDD280 pKa = 3.65FYY282 pKa = 11.23VYY284 pKa = 10.59DD285 pKa = 4.98IITPGLGGSATDD297 pKa = 5.45LISIQANSTMYY308 pKa = 9.35WHH310 pKa = 7.07EE311 pKa = 4.05KK312 pKa = 9.5

Molecular weight: 35.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

631

312

319

315.5

35.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.448 ± 0.517

1.585 ± 0.218

6.656 ± 1.561

3.645 ± 1.009

6.498 ± 1.678

8.399 ± 0.278

2.06 ± 0.56

5.388 ± 0.418

4.437 ± 0.959

5.864 ± 0.76

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.694 ± 0.829

4.754 ± 1.423

4.12 ± 0.428

2.853 ± 0.946

7.607 ± 0.4

7.607 ± 1.677

5.864 ± 1.778

6.022 ± 0.182

2.694 ± 0.094

3.803 ± 0.492
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski