Bacillus sp. P16(2019)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4393 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A554A316|A0A554A316_9BACI GAF domain-containing protein OS=Bacillus sp. P16(2019) OX=2597270 GN=FN960_00485 PE=4 SV=1
MM1 pKa = 7.29NLSNKK6 pKa = 8.5WFVGVSLSAIVLVTGCSNEE25 pKa = 3.7GSGDD29 pKa = 3.57GDD31 pKa = 3.65SDD33 pKa = 3.44QTTIRR38 pKa = 11.84VHH40 pKa = 4.99HH41 pKa = 7.26WYY43 pKa = 10.57NEE45 pKa = 4.06EE46 pKa = 3.54QDD48 pKa = 3.33NWDD51 pKa = 3.74EE52 pKa = 4.02VFAAYY57 pKa = 9.97EE58 pKa = 4.15EE59 pKa = 4.28EE60 pKa = 4.03TGIKK64 pKa = 9.46IEE66 pKa = 4.74SVTPEE71 pKa = 3.92NNDD74 pKa = 3.35ANEE77 pKa = 4.02TLQQIDD83 pKa = 3.62LAAASGDD90 pKa = 3.63QLDD93 pKa = 4.21VIMVNDD99 pKa = 3.93AANYY103 pKa = 7.2SQRR106 pKa = 11.84ISQGMFEE113 pKa = 4.8PLNDD117 pKa = 3.86YY118 pKa = 10.92LDD120 pKa = 3.97EE121 pKa = 6.59AGIQYY126 pKa = 10.85EE127 pKa = 4.88DD128 pKa = 5.2DD129 pKa = 3.98YY130 pKa = 12.1QNDD133 pKa = 3.77TSVDD137 pKa = 3.33NQYY140 pKa = 11.13YY141 pKa = 10.69ALPGKK146 pKa = 9.84YY147 pKa = 9.11NQSFVMLNEE156 pKa = 4.52DD157 pKa = 3.91ALDD160 pKa = 3.92EE161 pKa = 4.95AGLDD165 pKa = 4.45IPVDD169 pKa = 3.98WTWDD173 pKa = 3.36DD174 pKa = 3.65YY175 pKa = 11.5MDD177 pKa = 3.9YY178 pKa = 11.53AEE180 pKa = 5.62ALTEE184 pKa = 4.28GEE186 pKa = 4.57GSSKK190 pKa = 10.63RR191 pKa = 11.84YY192 pKa = 8.39GTFFHH197 pKa = 6.25TWVDD201 pKa = 3.99YY202 pKa = 11.5VKK204 pKa = 10.4LAAMNQSEE212 pKa = 4.05QSNLVQDD219 pKa = 4.94DD220 pKa = 4.46GVTSNINSDD229 pKa = 4.25IIRR232 pKa = 11.84QSLEE236 pKa = 3.14IRR238 pKa = 11.84QRR240 pKa = 11.84GIDD243 pKa = 3.63EE244 pKa = 4.85GSATPHH250 pKa = 7.16ADD252 pKa = 3.85TISLDD257 pKa = 3.62LNYY260 pKa = 10.26RR261 pKa = 11.84PQFFSEE267 pKa = 4.29SAAMIMTGSWMINEE281 pKa = 4.16AGGRR285 pKa = 11.84PEE287 pKa = 3.94EE288 pKa = 4.34PSTFRR293 pKa = 11.84TAFAPYY299 pKa = 9.78PKK301 pKa = 10.76ANDD304 pKa = 3.56NDD306 pKa = 5.54PITTPAGLDD315 pKa = 3.45FLTVYY320 pKa = 10.41SGSDD324 pKa = 3.15HH325 pKa = 6.19KK326 pKa = 11.36QEE328 pKa = 4.31AFDD331 pKa = 4.41FIRR334 pKa = 11.84WYY336 pKa = 6.68TTEE339 pKa = 5.24GILDD343 pKa = 3.39QGMYY347 pKa = 10.23YY348 pKa = 9.83PDD350 pKa = 4.17YY351 pKa = 10.95KK352 pKa = 11.05NADD355 pKa = 3.27IDD357 pKa = 4.23TVLDD361 pKa = 3.72NLLDD365 pKa = 3.68NTPNIEE371 pKa = 4.11MVNRR375 pKa = 11.84DD376 pKa = 3.48SLQATLEE383 pKa = 4.11ASEE386 pKa = 4.46PADD389 pKa = 3.37MNIPVPYY396 pKa = 9.37IGEE399 pKa = 4.19VVTAYY404 pKa = 10.26MNEE407 pKa = 3.9TDD409 pKa = 4.18RR410 pKa = 11.84FLLEE414 pKa = 4.2EE415 pKa = 4.18QDD417 pKa = 4.32LEE419 pKa = 4.58TTIEE423 pKa = 3.91RR424 pKa = 11.84AEE426 pKa = 4.17EE427 pKa = 4.12SVQQVIDD434 pKa = 3.59NNANN438 pKa = 3.09

Molecular weight: 49.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A553ZSJ3|A0A553ZSJ3_9BACI EAL domain-containing protein (Fragment) OS=Bacillus sp. P16(2019) OX=2597270 GN=FN960_21955 PE=4 SV=1
RR1 pKa = 7.24ARR3 pKa = 11.84PVRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84SGSRR12 pKa = 11.84GARR15 pKa = 11.84RR16 pKa = 11.84PGRR19 pKa = 11.84AAGAPSSARR28 pKa = 11.84TRR30 pKa = 11.84RR31 pKa = 11.84PAPAAPPRR39 pKa = 11.84GAPGRR44 pKa = 11.84PAPRR48 pKa = 11.84WSAPAGSGRR57 pKa = 11.84RR58 pKa = 11.84RR59 pKa = 11.84AGRR62 pKa = 11.84PSRR65 pKa = 11.84PGPGARR71 pKa = 11.84PRR73 pKa = 11.84RR74 pKa = 11.84RR75 pKa = 11.84GRR77 pKa = 11.84CGRR80 pKa = 11.84TGG82 pKa = 2.91

Molecular weight: 8.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4393

0

4393

1227302

21

2698

279.4

31.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.289 ± 0.04

0.673 ± 0.011

5.201 ± 0.033

7.659 ± 0.045

4.409 ± 0.035

6.853 ± 0.039

2.284 ± 0.02

7.41 ± 0.034

5.678 ± 0.038

10.139 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.754 ± 0.019

3.984 ± 0.031

3.642 ± 0.022

4.219 ± 0.028

4.207 ± 0.032

6.373 ± 0.027

5.665 ± 0.024

7.01 ± 0.031

1.097 ± 0.014

3.453 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski