Clostridium phage HM T

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L2BY98|A0A1L2BY98_9CAUD Head-tail adaptor OS=Clostridium phage HM T OX=1779843 GN=HMT_31 PE=4 SV=1
MM1 pKa = 8.11DD2 pKa = 5.97IIEE5 pKa = 5.34KK6 pKa = 10.05IDD8 pKa = 3.44IALSNALDD16 pKa = 4.01PLNIRR21 pKa = 11.84SFYY24 pKa = 10.5GWYY27 pKa = 10.23DD28 pKa = 3.28EE29 pKa = 5.94DD30 pKa = 4.07INGTHH35 pKa = 5.3VTFTLISDD43 pKa = 4.37LDD45 pKa = 3.55EE46 pKa = 6.48DD47 pKa = 4.4YY48 pKa = 11.71ADD50 pKa = 5.55DD51 pKa = 4.02EE52 pKa = 4.99AEE54 pKa = 4.17STTTLFQVDD63 pKa = 3.05IWSKK67 pKa = 11.35EE68 pKa = 3.64NMEE71 pKa = 4.34EE72 pKa = 3.89LKK74 pKa = 9.69KK75 pKa = 9.4TIKK78 pKa = 10.51SAMKK82 pKa = 9.64TLDD85 pKa = 3.15NCTYY89 pKa = 11.16NSGADD94 pKa = 3.49LYY96 pKa = 11.47EE97 pKa = 4.78NDD99 pKa = 3.13TKK101 pKa = 10.6IYY103 pKa = 10.53HH104 pKa = 6.18KK105 pKa = 10.57ALRR108 pKa = 11.84FNITQEE114 pKa = 4.09VTT116 pKa = 3.02

Molecular weight: 13.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L2BY88|A0A1L2BY88_9CAUD Uncharacterized protein OS=Clostridium phage HM T OX=1779843 GN=HMT_21 PE=4 SV=1
MM1 pKa = 7.28GRR3 pKa = 11.84KK4 pKa = 9.0RR5 pKa = 11.84PAKK8 pKa = 9.79PIVRR12 pKa = 11.84TDD14 pKa = 3.34QVLDD18 pKa = 3.22IQDD21 pKa = 3.48YY22 pKa = 10.6LKK24 pKa = 10.78YY25 pKa = 10.43RR26 pKa = 11.84NEE28 pKa = 3.56RR29 pKa = 11.84DD30 pKa = 3.38YY31 pKa = 11.48ILFVVGITTGYY42 pKa = 9.91RR43 pKa = 11.84AGDD46 pKa = 3.91LVTLKK51 pKa = 10.87ARR53 pKa = 11.84DD54 pKa = 3.26IRR56 pKa = 11.84EE57 pKa = 4.05ALKK60 pKa = 10.69KK61 pKa = 10.6SEE63 pKa = 3.83FTIFEE68 pKa = 4.32GKK70 pKa = 9.8KK71 pKa = 10.15KK72 pKa = 9.72NSKK75 pKa = 10.3NIRR78 pKa = 11.84EE79 pKa = 4.39KK80 pKa = 10.28NRR82 pKa = 11.84KK83 pKa = 7.61PRR85 pKa = 11.84TVEE88 pKa = 4.09IIPKK92 pKa = 8.94VAKK95 pKa = 10.1LLKK98 pKa = 10.29EE99 pKa = 4.08YY100 pKa = 10.46IRR102 pKa = 11.84DD103 pKa = 3.73KK104 pKa = 11.14KK105 pKa = 10.59DD106 pKa = 3.06YY107 pKa = 10.59EE108 pKa = 4.55YY109 pKa = 10.65IFQSRR114 pKa = 11.84KK115 pKa = 9.7GVNKK119 pKa = 10.24HH120 pKa = 6.04IGVQAVSNILKK131 pKa = 10.17EE132 pKa = 3.97AGEE135 pKa = 4.28YY136 pKa = 10.29FGLYY140 pKa = 10.02DD141 pKa = 4.16ISAHH145 pKa = 6.0SMRR148 pKa = 11.84KK149 pKa = 6.96TYY151 pKa = 10.33AYY153 pKa = 9.51KK154 pKa = 10.17IYY156 pKa = 10.3IEE158 pKa = 4.09SDD160 pKa = 2.78KK161 pKa = 11.39DD162 pKa = 3.19IVAVQKK168 pKa = 11.1LLGHH172 pKa = 6.56RR173 pKa = 11.84TTQEE177 pKa = 3.62TEE179 pKa = 4.05LYY181 pKa = 10.23IGLDD185 pKa = 3.17KK186 pKa = 11.01EE187 pKa = 5.31KK188 pKa = 9.94YY189 pKa = 7.96HH190 pKa = 6.49QYY192 pKa = 10.95SKK194 pKa = 11.12SLSDD198 pKa = 3.62FVRR201 pKa = 4.72

Molecular weight: 23.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

11199

42

1069

233.3

26.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.179 ± 0.495

1.072 ± 0.176

6.635 ± 0.295

7.572 ± 0.496

3.965 ± 0.249

5.733 ± 0.45

0.911 ± 0.128

9.09 ± 0.376

9.287 ± 0.497

8.153 ± 0.27

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.402 ± 0.165

8.144 ± 0.325

2.116 ± 0.193

3.018 ± 0.227

3.098 ± 0.393

6.76 ± 0.368

5.858 ± 0.534

5.474 ± 0.336

1.063 ± 0.184

4.474 ± 0.407
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski