bacterium HR29

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2317 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5ZE43|A0A2H5ZE43_9BACT Uncharacterized protein OS=bacterium HR29 OX=2035424 GN=HRbin29_01628 PE=4 SV=1
MM1 pKa = 7.61LLCLLSGFACSHH13 pKa = 6.69SDD15 pKa = 3.64PNSDD19 pKa = 3.07TDD21 pKa = 4.05TNAHH25 pKa = 6.48ANADD29 pKa = 4.14SQPHH33 pKa = 6.95PDD35 pKa = 3.84SDD37 pKa = 3.95SHH39 pKa = 6.55TDD41 pKa = 4.15AADD44 pKa = 2.92GWAASGRR51 pKa = 11.84SAGPYY56 pKa = 10.25AEE58 pKa = 4.96LSEE61 pKa = 4.77DD62 pKa = 3.54AAPP65 pKa = 4.21

Molecular weight: 6.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5ZF64|A0A2H5ZF64_9BACT Sensor kinase CckA OS=bacterium HR29 OX=2035424 GN=cckA_4 PE=4 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.08RR4 pKa = 11.84TFQPHH9 pKa = 3.81NRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84FRR15 pKa = 11.84VHH17 pKa = 6.5GFRR20 pKa = 11.84ARR22 pKa = 11.84MKK24 pKa = 8.69TRR26 pKa = 11.84AGRR29 pKa = 11.84LVLKK33 pKa = 10.52ARR35 pKa = 11.84RR36 pKa = 11.84LKK38 pKa = 10.07GRR40 pKa = 11.84KK41 pKa = 9.08RR42 pKa = 11.84LTVEE46 pKa = 3.7

Molecular weight: 5.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2317

0

2317

719333

29

1969

310.5

33.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.449 ± 0.083

0.861 ± 0.018

4.872 ± 0.029

7.202 ± 0.055

3.453 ± 0.031

8.736 ± 0.05

1.871 ± 0.021

4.196 ± 0.035

1.898 ± 0.032

10.456 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.816 ± 0.019

1.859 ± 0.026

6.214 ± 0.044

2.645 ± 0.034

9.159 ± 0.055

4.384 ± 0.036

4.741 ± 0.037

8.397 ± 0.046

1.544 ± 0.022

2.247 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski