Paenibacillus mucilaginosus 3016

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; Paenibacillus mucilaginosus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7018 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H6NNI5|H6NNI5_9BACL Uncharacterized protein OS=Paenibacillus mucilaginosus 3016 OX=1116391 GN=PM3016_6698 PE=4 SV=1
MM1 pKa = 8.0DD2 pKa = 6.05KK3 pKa = 11.06DD4 pKa = 3.58LTDD7 pKa = 3.96GSRR10 pKa = 11.84VSLWLGSWSSEE21 pKa = 4.05EE22 pKa = 3.61AAKK25 pKa = 10.56AYY27 pKa = 10.92VEE29 pKa = 4.21GSYY32 pKa = 10.16TEE34 pKa = 5.0DD35 pKa = 3.3GDD37 pKa = 4.27YY38 pKa = 11.17VPSPFEE44 pKa = 3.51RR45 pKa = 11.84DD46 pKa = 2.89FAFRR50 pKa = 11.84YY51 pKa = 10.52DD52 pKa = 3.56PDD54 pKa = 4.67FIEE57 pKa = 5.6LNFKK61 pKa = 7.54EE62 pKa = 4.68TPAADD67 pKa = 3.69WEE69 pKa = 4.62SLLQGHH75 pKa = 6.35SYY77 pKa = 11.09DD78 pKa = 4.27EE79 pKa = 4.61EE80 pKa = 5.44IIPAFMEE87 pKa = 4.73EE88 pKa = 4.26IGPLSRR94 pKa = 11.84SFNTVILVYY103 pKa = 10.74NLAYY107 pKa = 10.3DD108 pKa = 4.05AEE110 pKa = 4.5HH111 pKa = 6.54LRR113 pKa = 11.84SGTAAGEE120 pKa = 4.0LEE122 pKa = 4.76YY123 pKa = 10.3IGQAAYY129 pKa = 10.26DD130 pKa = 3.58

Molecular weight: 14.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H6NJ14|H6NJ14_9BACL Uncharacterized protein OS=Paenibacillus mucilaginosus 3016 OX=1116391 GN=PM3016_1870 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.81PNVRR10 pKa = 11.84KK11 pKa = 9.78RR12 pKa = 11.84SKK14 pKa = 9.51VHH16 pKa = 5.89GFRR19 pKa = 11.84KK20 pKa = 10.02RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.87VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.79VLSAA44 pKa = 4.05

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7018

0

7018

2302481

29

6525

328.1

36.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.412 ± 0.04

0.759 ± 0.008

4.805 ± 0.022

6.937 ± 0.035

3.898 ± 0.023

8.334 ± 0.034

2.105 ± 0.017

5.477 ± 0.03

4.472 ± 0.032

10.594 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.626 ± 0.017

2.99 ± 0.026

4.628 ± 0.02

3.671 ± 0.018

5.958 ± 0.036

6.054 ± 0.022

5.379 ± 0.026

7.083 ± 0.023

1.383 ± 0.012

3.434 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski