Candidatus Saccharibacteria bacterium RAAC3_TM7_1

Taxonomy: cellular organisms; Bacteria; Bacteria incertae sedis; Bacteria candidate phyla; Candidatus Saccharibacteria; unclassified Saccharibacteria

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 920 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5RUT5|V5RUT5_9BACT Flavodoxin/nitric oxide synthase OS=Candidatus Saccharibacteria bacterium RAAC3_TM7_1 OX=1394711 GN=RAAC3_TM7C00001G0130 PE=4 SV=1
MM1 pKa = 7.42LSIRR5 pKa = 11.84RR6 pKa = 11.84YY7 pKa = 7.85MLPGLSLILASLVFPIMFSTGAYY30 pKa = 9.66AVSQYY35 pKa = 11.68DD36 pKa = 3.23NAYY39 pKa = 10.43NHH41 pKa = 6.15VDD43 pKa = 3.23QLKK46 pKa = 10.0IVQNNYY52 pKa = 10.64NGVTCDD58 pKa = 3.86PLDD61 pKa = 3.15ITMTYY66 pKa = 10.48QSYY69 pKa = 11.18LNNSAKK75 pKa = 9.97WYY77 pKa = 6.12PTTSAFATTMQAYY90 pKa = 9.44KK91 pKa = 10.74SSFAQARR98 pKa = 11.84QAGSWTVSVVLVNDD112 pKa = 4.25GSGGYY117 pKa = 8.61TSIVRR122 pKa = 11.84INWFEE127 pKa = 4.48DD128 pKa = 3.74DD129 pKa = 3.66PGKK132 pKa = 10.74LVFKK136 pKa = 9.38GTSPNTQVEE145 pKa = 4.59YY146 pKa = 11.04QSTTDD151 pKa = 3.57TQGHH155 pKa = 5.47YY156 pKa = 11.11AFISTIDD163 pKa = 3.31FARR166 pKa = 11.84PYY168 pKa = 10.97GSTDD172 pKa = 3.07AGNCTPHH179 pKa = 7.4VYY181 pKa = 10.54AVGDD185 pKa = 4.1FVANANEE192 pKa = 3.7EE193 pKa = 4.42TYY195 pKa = 11.22AVVSTEE201 pKa = 4.68GAFSWTPTSSSEE213 pKa = 3.83MGHH216 pKa = 6.0WANLFATSTNVQYY229 pKa = 8.12PTGYY233 pKa = 9.89EE234 pKa = 3.99GAAISANSPTATYY247 pKa = 11.23VAMGDD252 pKa = 3.75SFSSGEE258 pKa = 3.97GNPPFEE264 pKa = 4.99YY265 pKa = 8.98GTDD268 pKa = 3.55SASDD272 pKa = 3.31KK273 pKa = 10.68CHH275 pKa = 6.98RR276 pKa = 11.84SPNAYY281 pKa = 8.87PRR283 pKa = 11.84LLQSDD288 pKa = 4.02SSLSLGSTAFVACSGATTDD307 pKa = 4.73DD308 pKa = 3.7VLNGGSGAGNWGEE321 pKa = 4.19GPQVDD326 pKa = 4.48ALLSDD331 pKa = 4.08TEE333 pKa = 4.59VVTITIGGNNIKK345 pKa = 10.21FAEE348 pKa = 4.42FAHH351 pKa = 6.26ACLFGSCNSSSDD363 pKa = 3.8EE364 pKa = 4.11YY365 pKa = 11.01QEE367 pKa = 4.32SWDD370 pKa = 3.63VMTDD374 pKa = 3.35LTRR377 pKa = 11.84SDD379 pKa = 4.27YY380 pKa = 11.27LPSQLDD386 pKa = 3.57SLFSDD391 pKa = 3.72IAFHH395 pKa = 6.72LWQNTSVKK403 pKa = 9.78VYY405 pKa = 9.96VVGYY409 pKa = 8.71PYY411 pKa = 10.96VITQASWNDD420 pKa = 3.43RR421 pKa = 11.84GVGICSDD428 pKa = 3.77FDD430 pKa = 3.82EE431 pKa = 6.41DD432 pKa = 3.68EE433 pKa = 5.37AIAAEE438 pKa = 4.47TIVSKK443 pKa = 10.97LDD445 pKa = 3.55TVIEE449 pKa = 4.16TAVTDD454 pKa = 3.96FDD456 pKa = 4.33DD457 pKa = 3.97SRR459 pKa = 11.84FVYY462 pKa = 10.41VDD464 pKa = 3.55PLATGSPFLGHH475 pKa = 5.81EE476 pKa = 4.31LCRR479 pKa = 11.84NGSYY483 pKa = 10.93FNGIEE488 pKa = 4.0TSGALGGDD496 pKa = 3.81DD497 pKa = 4.91AYY499 pKa = 11.65VFHH502 pKa = 8.09PDD504 pKa = 3.13TNGQQAYY511 pKa = 10.19ADD513 pKa = 5.12LIRR516 pKa = 11.84SYY518 pKa = 10.8MSS520 pKa = 3.07

Molecular weight: 56.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5RVU6|V5RVU6_9BACT Fimbrial protein pilin OS=Candidatus Saccharibacteria bacterium RAAC3_TM7_1 OX=1394711 GN=RAAC3_TM7C00001G0659 PE=4 SV=1
MM1 pKa = 7.84PKK3 pKa = 10.55LKK5 pKa = 8.66THH7 pKa = 6.85KK8 pKa = 9.66GTAKK12 pKa = 9.88RR13 pKa = 11.84VKK15 pKa = 8.75ITGSGKK21 pKa = 8.17ITRR24 pKa = 11.84QRR26 pKa = 11.84ASGGHH31 pKa = 6.27LLAKK35 pKa = 9.92KK36 pKa = 10.13SKK38 pKa = 9.24SRR40 pKa = 11.84KK41 pKa = 8.36RR42 pKa = 11.84AINSTGAITGRR53 pKa = 11.84IAKK56 pKa = 9.64NIKK59 pKa = 9.07QALGAKK65 pKa = 9.35

Molecular weight: 6.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

920

0

920

255811

30

1341

278.1

30.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.995 ± 0.089

0.602 ± 0.028

5.698 ± 0.072

6.273 ± 0.097

3.647 ± 0.058

7.011 ± 0.072

2.112 ± 0.046

6.468 ± 0.059

5.924 ± 0.088

9.67 ± 0.096

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.27 ± 0.036

3.717 ± 0.06

3.963 ± 0.052

3.736 ± 0.047

5.47 ± 0.079

6.327 ± 0.08

6.216 ± 0.081

7.329 ± 0.072

1.112 ± 0.035

3.458 ± 0.055
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski