Mycoplasma iowae DK-CPA

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma; Mycoplasma iowae

Average proteome isoelectric point is 7.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 854 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A084U478|A0A084U478_MYCIO Uncharacterized protein OS=Mycoplasma iowae DK-CPA OX=1394179 GN=P271_623 PE=4 SV=1
MM1 pKa = 7.55EE2 pKa = 5.55LFEE5 pKa = 4.85KK6 pKa = 10.68LRR8 pKa = 11.84LIIKK12 pKa = 9.2NSKK15 pKa = 9.4FISIISIIYY24 pKa = 9.77IIMLALYY31 pKa = 10.1SVIIIINNQLRR42 pKa = 11.84SQNVHH47 pKa = 4.33VTTDD51 pKa = 3.45GEE53 pKa = 4.34IVINGPVQTLPVGIILFAQICLTIAITLIVLSSTLSINVFMKK95 pKa = 10.55LKK97 pKa = 10.2RR98 pKa = 11.84LSKK101 pKa = 10.69DD102 pKa = 3.22SVHH105 pKa = 6.07GQEE108 pKa = 5.26IYY110 pKa = 11.14DD111 pKa = 3.76NLIICSFLSIIFFGFIFSFILWIKK135 pKa = 8.7SVKK138 pKa = 9.62VSKK141 pKa = 10.71NLEE144 pKa = 3.78NYY146 pKa = 9.33YY147 pKa = 10.86DD148 pKa = 3.94YY149 pKa = 11.79VDD151 pKa = 3.29SLEE154 pKa = 4.14FNEE157 pKa = 5.35NEE159 pKa = 4.05VEE161 pKa = 4.29TKK163 pKa = 10.53KK164 pKa = 10.64RR165 pKa = 11.84FSFFKK170 pKa = 10.46RR171 pKa = 11.84FKK173 pKa = 9.91KK174 pKa = 10.67QKK176 pKa = 10.56DD177 pKa = 3.55DD178 pKa = 3.95FSNDD182 pKa = 3.33EE183 pKa = 4.23IDD185 pKa = 4.3DD186 pKa = 4.0NKK188 pKa = 10.67QIEE191 pKa = 4.5DD192 pKa = 3.34WNIRR196 pKa = 11.84DD197 pKa = 4.04DD198 pKa = 4.35DD199 pKa = 4.91YY200 pKa = 12.05YY201 pKa = 11.06MNSDD205 pKa = 3.18SYY207 pKa = 12.09YY208 pKa = 10.26NNQYY212 pKa = 10.13PDD214 pKa = 3.11NNKK217 pKa = 9.21TYY219 pKa = 11.17NSTQQSDD226 pKa = 4.06YY227 pKa = 11.34QEE229 pKa = 5.45SNDD232 pKa = 4.74LYY234 pKa = 11.5DD235 pKa = 5.35EE236 pKa = 5.25IYY238 pKa = 11.02DD239 pKa = 4.97DD240 pKa = 6.2DD241 pKa = 6.85DD242 pKa = 3.61DD243 pKa = 5.16TIYY246 pKa = 10.68HH247 pKa = 6.63YY248 pKa = 11.56DD249 pKa = 3.8NDD251 pKa = 3.72YY252 pKa = 11.65DD253 pKa = 5.46DD254 pKa = 5.36YY255 pKa = 12.02DD256 pKa = 3.86NEE258 pKa = 4.61PYY260 pKa = 10.45HH261 pKa = 7.63DD262 pKa = 5.8DD263 pKa = 3.52GLGYY267 pKa = 10.54YY268 pKa = 9.8QDD270 pKa = 3.55SQYY273 pKa = 11.67GDD275 pKa = 4.19NNDD278 pKa = 2.73HH279 pKa = 7.3RR280 pKa = 11.84YY281 pKa = 9.47

Molecular weight: 33.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A084U335|A0A084U335_MYCIO Protein translocase subunit SecY OS=Mycoplasma iowae DK-CPA OX=1394179 GN=secY PE=3 SV=1
MM1 pKa = 7.44ARR3 pKa = 11.84ILGVDD8 pKa = 3.26IPNNKK13 pKa = 9.09RR14 pKa = 11.84VVISLTYY21 pKa = 9.53VYY23 pKa = 10.79GIGRR27 pKa = 11.84SRR29 pKa = 11.84SSEE32 pKa = 3.58ILAKK36 pKa = 10.71AKK38 pKa = 9.82IDD40 pKa = 3.67EE41 pKa = 4.14NRR43 pKa = 11.84KK44 pKa = 9.66VSTLTDD50 pKa = 3.41EE51 pKa = 4.41EE52 pKa = 4.82LATIRR57 pKa = 11.84KK58 pKa = 8.52IAAEE62 pKa = 3.78YY63 pKa = 9.26MIEE66 pKa = 4.21GDD68 pKa = 4.07LRR70 pKa = 11.84RR71 pKa = 11.84EE72 pKa = 3.44TAMNIKK78 pKa = 10.02RR79 pKa = 11.84LMEE82 pKa = 4.29IGCYY86 pKa = 9.42RR87 pKa = 11.84GVRR90 pKa = 11.84HH91 pKa = 6.17RR92 pKa = 11.84RR93 pKa = 11.84SLPVRR98 pKa = 11.84GQRR101 pKa = 11.84TKK103 pKa = 11.26SNARR107 pKa = 11.84TRR109 pKa = 11.84KK110 pKa = 9.46GPRR113 pKa = 11.84KK114 pKa = 7.53TVANKK119 pKa = 10.28KK120 pKa = 9.63IEE122 pKa = 4.32SKK124 pKa = 11.03

Molecular weight: 14.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

854

0

854

325412

27

2273

381.0

43.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.14 ± 0.074

0.774 ± 0.028

5.861 ± 0.064

6.114 ± 0.103

5.49 ± 0.069

4.789 ± 0.088

1.149 ± 0.03

9.956 ± 0.086

10.269 ± 0.097

8.827 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.854 ± 0.03

8.997 ± 0.109

2.6 ± 0.045

2.852 ± 0.042

2.352 ± 0.046

7.399 ± 0.092

5.428 ± 0.091

5.74 ± 0.062

0.939 ± 0.027

4.47 ± 0.067
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski