Methylobacterium sp. YIM 132548

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Methylobacterium; unclassified Methylobacterium

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5304 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N6MJM9|A0A6N6MJM9_9RHIZ Acyl-CoA dehydrogenase OS=Methylobacterium sp. YIM 132548 OX=2615211 GN=F6X51_23340 PE=3 SV=1
AA1 pKa = 7.63FSVTPGTALGDD12 pKa = 3.48GPYY15 pKa = 10.88AFTATATDD23 pKa = 3.35AAGNLGAPSAPVSLTIDD40 pKa = 3.61TAVPAAPVVTSAGGATSDD58 pKa = 3.48TTPAITGTGEE68 pKa = 3.74AGATVTLLDD77 pKa = 3.34GTAPVGTATVGQNGGFTVSPGAPLGEE103 pKa = 4.69GPHH106 pKa = 5.93NLSVQLTDD114 pKa = 3.14AAGNVGAPSPAVAVTIDD131 pKa = 3.75SLAPTIPVVTGGGGATADD149 pKa = 3.52TTPAVTGTAEE159 pKa = 4.17PGATVTLLNGTTALGTATVNPDD181 pKa = 2.55GSFTVSPTTPLGQGTYY197 pKa = 9.87DD198 pKa = 3.46LSVRR202 pKa = 11.84VTDD205 pKa = 3.87AAGNTSPVTAAIGVTVDD222 pKa = 3.23TTLPTTPTLTAPAGPTSDD240 pKa = 3.61STPTLTGTAEE250 pKa = 4.13ANSTVTIANGNTVLGTVTADD270 pKa = 2.85SGGAFSFTPVPAFGDD285 pKa = 3.4GTYY288 pKa = 11.45ALTATVRR295 pKa = 11.84DD296 pKa = 3.39AAGNVGAPSAPVSLTIDD313 pKa = 3.35TAAPVMPVLTSAGGPTSSAAPAITGTGEE341 pKa = 3.06IGARR345 pKa = 11.84VTLLDD350 pKa = 3.17GTAPVGTAIVDD361 pKa = 3.42QDD363 pKa = 3.44GSFTVSPGAPLGEE376 pKa = 4.69GPHH379 pKa = 5.93NLSVQLTDD387 pKa = 3.14AAGNVGAPSMAITVTVDD404 pKa = 2.78VTAPAAPVFTTASGVTADD422 pKa = 4.31ATPAIAGTAEE432 pKa = 3.99AGATVTVFDD441 pKa = 5.57DD442 pKa = 4.03GTPLGTVQANARR454 pKa = 11.84GAWSFTPGTALANGPNLITATATDD478 pKa = 3.72AVGNISPAATGPTLTVDD495 pKa = 3.91TGAPPAPVLTIAPGPTNDD513 pKa = 3.54NTPTITGTAEE523 pKa = 3.8ANSTVTITSGGILIATVTADD543 pKa = 2.89AGGAFSVTPGTALGDD558 pKa = 3.48GPYY561 pKa = 10.88AFTATATDD569 pKa = 3.35AAGNLGAPSAPVSLTIDD586 pKa = 3.61TAVPAAPVVTSAGGATSDD604 pKa = 3.48TTPAITGTGEE614 pKa = 3.74AGATVTLLDD623 pKa = 3.34GTAPVGTATVGQNGGFTVSPGAPLGEE649 pKa = 4.69GPHH652 pKa = 6.09NLSVHH657 pKa = 5.55LTDD660 pKa = 4.04AAGNVGAPSPAVAVTVDD677 pKa = 3.68TNVDD681 pKa = 3.65VPPALAFTVATTADD695 pKa = 3.17GRR697 pKa = 11.84LNATEE702 pKa = 4.13AQATAFTIAGLDD714 pKa = 3.29AGTTGTATFTDD725 pKa = 3.8ASTASVSVAIGANGTYY741 pKa = 10.52AADD744 pKa = 3.94LSGLDD749 pKa = 3.84GPVTTSLRR757 pKa = 11.84VTDD760 pKa = 3.9PAGNAGTIAGPSLTLDD776 pKa = 3.19TLAPVGTAVADD787 pKa = 3.77ASGGPEE793 pKa = 3.87VASFTYY799 pKa = 9.52TVSFPEE805 pKa = 3.88AVANVGAEE813 pKa = 4.07DD814 pKa = 3.84FTLTGTNGATGTIAGVTGSGGTYY837 pKa = 8.04TVTVAEE843 pKa = 4.23VSGAGTLSLGLAAGSDD859 pKa = 3.54ITDD862 pKa = 3.85LAGNAASLTPADD874 pKa = 3.75RR875 pKa = 11.84AVIGVAPVLPVITGYY890 pKa = 9.3TIDD893 pKa = 3.91TGVPSDD899 pKa = 5.27GITGDD904 pKa = 3.63PTPTLTGIGAAGSTVTVTYY923 pKa = 8.86ATAAGPAQATGTVLADD939 pKa = 3.89GTWSLTLPTLADD951 pKa = 3.29GDD953 pKa = 4.36YY954 pKa = 11.33SFTASLATAGGTPIGTSAPLALTIDD979 pKa = 3.83TGVDD983 pKa = 2.69AGTPTTLVVDD993 pKa = 4.55GTADD997 pKa = 3.55GLINAAEE1004 pKa = 4.18ATAVAYY1010 pKa = 8.09TVAGLDD1016 pKa = 3.33AGTSGTITFRR1026 pKa = 11.84DD1027 pKa = 5.03GIRR1030 pKa = 11.84ATEE1033 pKa = 4.08VAVSQNGTFAVDD1045 pKa = 3.49LSGFSGPVSANLALRR1060 pKa = 11.84DD1061 pKa = 3.8VAGNAATLAGNTVSLATGAPGAPVITGLADD1091 pKa = 3.39DD1092 pKa = 4.78TGTLGDD1098 pKa = 4.4TLTADD1103 pKa = 3.88TNPTLSGLADD1113 pKa = 4.21PGSAIRR1119 pKa = 11.84LDD1121 pKa = 3.71FTTAAGPQFVEE1132 pKa = 5.16TVAGASGAWTATLPLLPDD1150 pKa = 3.74GAYY1153 pKa = 10.38AVTASARR1160 pKa = 11.84DD1161 pKa = 3.43AAGNASVASAPFPISIDD1178 pKa = 3.08ATAPAGTAGADD1189 pKa = 3.46TAGGLTAEE1197 pKa = 4.45TFTYY1201 pKa = 9.7TVSFPEE1207 pKa = 3.88AVANVGAEE1215 pKa = 4.16DD1216 pKa = 4.6FSLTGTNGATGTIAGVTGSGGTYY1239 pKa = 7.89TVTVAGVSGAGTLSLGLAAGSDD1261 pKa = 3.23IADD1264 pKa = 3.9RR1265 pKa = 11.84AGNLASLTPATRR1277 pKa = 11.84NVAVGPDD1284 pKa = 3.32ATVPTTISGFTEE1296 pKa = 4.37DD1297 pKa = 4.53SGVAGDD1303 pKa = 5.62GITSDD1308 pKa = 3.75ATPTLTGTGNAGGTVTVTFATAAGAQTLTGPVDD1341 pKa = 3.95ALGNWTVPVPEE1352 pKa = 4.87LADD1355 pKa = 3.31GRR1357 pKa = 11.84YY1358 pKa = 10.03SFTATTSGPSGAPGGTSAPLALTIDD1383 pKa = 4.12TVADD1387 pKa = 3.84ALPAVALALDD1397 pKa = 4.68GSADD1401 pKa = 3.68GVLTPAEE1408 pKa = 4.0AATARR1413 pKa = 11.84FTLSGLDD1420 pKa = 3.52AGSGAAVTFTDD1431 pKa = 3.67AAGTRR1436 pKa = 11.84VAATASADD1444 pKa = 3.25GTYY1447 pKa = 10.03TVDD1450 pKa = 3.63LSGLIGPVTSSVVVTDD1466 pKa = 3.74AAGNSAVGSGNPIVIDD1482 pKa = 4.02AGTTTPPRR1490 pKa = 11.84TPVPTITGIQEE1501 pKa = 4.03DD1502 pKa = 4.46TGTPGDD1508 pKa = 5.07GITSDD1513 pKa = 3.77ASPILVGTASPGSTVTVSYY1532 pKa = 10.14TGAAGGAATATGTVGTDD1549 pKa = 3.71GAWQIALPALADD1561 pKa = 3.69GSYY1564 pKa = 11.01SFVATALAPGSTSSPASQALPVVIDD1589 pKa = 3.76TVVPAAPVFGGIVGDD1604 pKa = 4.6DD1605 pKa = 3.59GDD1607 pKa = 4.75GVVTDD1612 pKa = 5.13ASPTLTGTAEE1622 pKa = 4.31PGSTVTVTYY1631 pKa = 7.42EE1632 pKa = 4.19TPQGPGSATGTTGTDD1647 pKa = 3.37GRR1649 pKa = 11.84WTVDD1653 pKa = 3.06IPTLPDD1659 pKa = 3.11GSYY1662 pKa = 10.83SLTVGTTDD1670 pKa = 3.18AAGNAGAPAAPVSLTINTGTGGGGTGGGGTGGGGTGGGSTGGGGTGGDD1718 pKa = 3.8GGTGGSGGSGGSGGTAPVLVGDD1740 pKa = 4.64LNQTLAHH1747 pKa = 6.46APAVSGNLLANDD1759 pKa = 3.74TGAALKK1765 pKa = 8.96VTAVQFSGGLPVAVPTEE1782 pKa = 4.13GTVKK1786 pKa = 10.27VVSDD1790 pKa = 4.02HH1791 pKa = 5.83GTLTVSADD1799 pKa = 3.06GSYY1802 pKa = 10.53SYY1804 pKa = 10.89QAIGANNLDD1813 pKa = 3.67DD1814 pKa = 3.9TVRR1817 pKa = 11.84TAEE1820 pKa = 4.09HH1821 pKa = 5.4FTYY1824 pKa = 10.23TVTDD1828 pKa = 3.28AAGAVGQSSLDD1839 pKa = 3.3ISLGGQAPQASASFGFAFTEE1859 pKa = 4.23ARR1861 pKa = 11.84VTLAGEE1867 pKa = 4.14ALVLVGPDD1875 pKa = 3.04GVTRR1879 pKa = 11.84DD1880 pKa = 3.08ISGIDD1885 pKa = 3.24TLRR1888 pKa = 11.84FTDD1891 pKa = 5.98GEE1893 pKa = 4.1IQNNDD1898 pKa = 2.76GHH1900 pKa = 7.09HH1901 pKa = 5.99VVDD1904 pKa = 5.03DD1905 pKa = 3.66VFYY1908 pKa = 10.62YY1909 pKa = 11.16ANNLDD1914 pKa = 3.19VWRR1917 pKa = 11.84AHH1919 pKa = 6.07VDD1921 pKa = 2.97ADD1923 pKa = 3.56THH1925 pKa = 6.61YY1926 pKa = 9.77EE1927 pKa = 3.98TFGWKK1932 pKa = 9.75EE1933 pKa = 3.67GRR1935 pKa = 11.84DD1936 pKa = 3.63PNAYY1940 pKa = 9.38FHH1942 pKa = 6.25TRR1944 pKa = 11.84AYY1946 pKa = 10.45LAEE1949 pKa = 4.43NPDD1952 pKa = 3.21VAAANINPLEE1962 pKa = 4.59HH1963 pKa = 6.65YY1964 pKa = 8.81LTFGEE1969 pKa = 4.35RR1970 pKa = 11.84EE1971 pKa = 4.18GRR1973 pKa = 11.84SPSPGFSAEE1982 pKa = 4.42AYY1984 pKa = 9.85LHH1986 pKa = 6.6ANPDD1990 pKa = 3.16VAAAGINALAHH2001 pKa = 6.02YY2002 pKa = 7.44LTNGQDD2008 pKa = 3.03EE2009 pKa = 4.65GRR2011 pKa = 11.84TVFAGEE2017 pKa = 4.12SEE2019 pKa = 4.53GPGRR2023 pKa = 11.84VIGDD2027 pKa = 3.85FDD2029 pKa = 5.77ADD2031 pKa = 4.43FYY2033 pKa = 11.05LAQYY2037 pKa = 10.57ADD2039 pKa = 3.38VAAAVPAGRR2048 pKa = 11.84EE2049 pKa = 3.8AASYY2053 pKa = 10.92ALEE2056 pKa = 4.66HH2057 pKa = 6.48YY2058 pKa = 8.73LTFGARR2064 pKa = 11.84EE2065 pKa = 3.95LRR2067 pKa = 11.84NPNAAFDD2074 pKa = 3.65TAYY2077 pKa = 10.97YY2078 pKa = 9.65LQQNPEE2084 pKa = 4.01VAASGANPLLHH2095 pKa = 5.49YY2096 pKa = 10.76QEE2098 pKa = 5.03VGWHH2102 pKa = 5.97EE2103 pKa = 4.01GRR2105 pKa = 11.84NPSASFNTNAYY2116 pKa = 8.95LASHH2120 pKa = 7.15PEE2122 pKa = 3.86VAQAGIDD2129 pKa = 3.77PLEE2132 pKa = 5.33HH2133 pKa = 6.1YY2134 pKa = 10.11LQTGGHH2140 pKa = 6.66ALAA2143 pKa = 5.38

Molecular weight: 205.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N6MSD0|A0A6N6MSD0_9RHIZ Transglutaminase-like cysteine peptidase OS=Methylobacterium sp. YIM 132548 OX=2615211 GN=F6X51_07385 PE=4 SV=1
MM1 pKa = 7.92RR2 pKa = 11.84LPLAGLGLFLPLAAWIAAAALVGGSAIATAPGASAQRR39 pKa = 11.84TVTPPAGQPRR49 pKa = 11.84VTAPGGRR56 pKa = 11.84RR57 pKa = 11.84GRR59 pKa = 11.84AAHH62 pKa = 5.75HH63 pKa = 6.21HH64 pKa = 6.26RR65 pKa = 11.84PAHH68 pKa = 5.06RR69 pKa = 11.84HH70 pKa = 3.94RR71 pKa = 5.6

Molecular weight: 7.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5304

0

5304

1615088

19

2478

304.5

32.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.355 ± 0.056

0.821 ± 0.01

5.438 ± 0.026

5.458 ± 0.031

3.369 ± 0.021

9.23 ± 0.033

1.948 ± 0.015

4.491 ± 0.023

2.385 ± 0.033

10.478 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.008 ± 0.016

2.116 ± 0.018

5.934 ± 0.034

2.769 ± 0.019

8.294 ± 0.037

4.949 ± 0.027

5.299 ± 0.033

7.427 ± 0.025

1.222 ± 0.014

2.009 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski