Synechococcus phage S-SM2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Bellamyvirus; unclassified Bellamyvirus

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 267 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3SJE7|E3SJE7_9CAUD Uncharacterized protein OS=Synechococcus phage S-SM2 OX=444860 GN=SSM2_262 PE=4 SV=1
MM1 pKa = 7.39LAVTLTIGTLVSITMFFVGGMVGWLAKK28 pKa = 9.29EE29 pKa = 4.19HH30 pKa = 6.52VYY32 pKa = 8.46QTQPVYY38 pKa = 9.48THH40 pKa = 7.25PEE42 pKa = 3.81MFDD45 pKa = 3.28EE46 pKa = 4.79NGNVLPDD53 pKa = 4.16EE54 pKa = 4.29ILAVRR59 pKa = 11.84FEE61 pKa = 4.43NDD63 pKa = 3.09YY64 pKa = 11.26EE65 pKa = 4.27PNEE68 pKa = 4.81DD69 pKa = 4.01YY70 pKa = 11.33DD71 pKa = 4.61EE72 pKa = 4.55EE73 pKa = 4.47DD74 pKa = 3.08

Molecular weight: 8.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3SJD8|E3SJD8_9CAUD Photosystem II D1 protein OS=Synechococcus phage S-SM2 OX=444860 GN=psbA PE=3 SV=1
MM1 pKa = 7.3MLLPYY6 pKa = 9.56MSEE9 pKa = 3.94PATGSRR15 pKa = 11.84MPSMSTGGAAMKK27 pKa = 9.56ATMKK31 pKa = 10.64QIVAANRR38 pKa = 11.84VGIIRR43 pKa = 11.84TPNQPTT49 pKa = 3.15

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

267

0

267

61413

30

4156

230.0

25.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.248 ± 0.203

0.977 ± 0.086

6.652 ± 0.119

6.694 ± 0.262

4.372 ± 0.106

7.712 ± 0.321

1.718 ± 0.1

6.363 ± 0.134

6.541 ± 0.341

7.194 ± 0.138

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.317 ± 0.152

5.561 ± 0.157

3.989 ± 0.141

3.476 ± 0.12

4.276 ± 0.132

6.865 ± 0.223

6.911 ± 0.388

6.878 ± 0.195

1.174 ± 0.102

4.082 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski