Klebsiella phage vB_KleM_RaK2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Alcyoneusvirus; Klebsiella virus RaK2

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 534 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H6X439|H6X439_9CAUD Uncharacterized protein OS=Klebsiella phage vB_KleM_RaK2 OX=1147094 GN=RaK2_00232 PE=4 SV=1
MM1 pKa = 7.77KK2 pKa = 10.07IKK4 pKa = 10.65CCVCDD9 pKa = 3.97CEE11 pKa = 4.94IDD13 pKa = 3.65EE14 pKa = 4.25KK15 pKa = 10.99HH16 pKa = 7.13VYY18 pKa = 10.39GEE20 pKa = 3.86WDD22 pKa = 4.72DD23 pKa = 4.07YY24 pKa = 11.96CSDD27 pKa = 3.35CWEE30 pKa = 4.22SEE32 pKa = 3.95GRR34 pKa = 11.84DD35 pKa = 3.43TEE37 pKa = 3.99RR38 pKa = 11.84WEE40 pKa = 5.91DD41 pKa = 3.58EE42 pKa = 4.59SEE44 pKa = 3.79WDD46 pKa = 3.67EE47 pKa = 5.42DD48 pKa = 3.95EE49 pKa = 4.31EE50 pKa = 4.63

Molecular weight: 6.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H6X4D5|H6X4D5_9CAUD Uncharacterized protein OS=Klebsiella phage vB_KleM_RaK2 OX=1147094 GN=RaK2_00328 PE=4 SV=1
MM1 pKa = 7.24ARR3 pKa = 11.84EE4 pKa = 4.01VNAGDD9 pKa = 3.07IKK11 pKa = 11.08AAVLRR16 pKa = 11.84TLKK19 pKa = 10.81AYY21 pKa = 9.39GRR23 pKa = 11.84KK24 pKa = 9.96LNDD27 pKa = 3.37RR28 pKa = 11.84NVPYY32 pKa = 10.78YY33 pKa = 10.48FLAATHH39 pKa = 6.15KK40 pKa = 10.0MAIAEE45 pKa = 4.03ARR47 pKa = 11.84GWLNDD52 pKa = 3.06RR53 pKa = 11.84STVKK57 pKa = 9.35STRR60 pKa = 11.84TRR62 pKa = 11.84NAIEE66 pKa = 3.81RR67 pKa = 11.84WIKK70 pKa = 9.78EE71 pKa = 4.02LNHH74 pKa = 6.29FF75 pKa = 4.52

Molecular weight: 8.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

534

0

534

104375

47

3326

195.5

22.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.095 ± 0.12

1.497 ± 0.073

6.479 ± 0.118

6.804 ± 0.155

4.62 ± 0.074

5.077 ± 0.136

1.882 ± 0.075

7.927 ± 0.116

7.664 ± 0.168

7.957 ± 0.124

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.632 ± 0.052

7.052 ± 0.121

2.874 ± 0.071

3.325 ± 0.078

3.448 ± 0.07

7.514 ± 0.132

6.221 ± 0.127

6.213 ± 0.099

1.052 ± 0.041

5.667 ± 0.122
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski