Rhodovulum sp. NI22

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Rhodovulum; unclassified Rhodovulum

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3439 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A095DSW4|A0A095DSW4_9RHOB Nudix hydrolase domain-containing protein OS=Rhodovulum sp. NI22 OX=1469613 GN=JT55_00930 PE=3 SV=1
MM1 pKa = 7.31KK2 pKa = 10.4RR3 pKa = 11.84IFLASAACLAVAACSGGDD21 pKa = 3.33GTNPITSGPTDD32 pKa = 3.69DD33 pKa = 5.23TEE35 pKa = 6.4DD36 pKa = 3.67PTTDD40 pKa = 3.24PTTDD44 pKa = 3.41PGTGIDD50 pKa = 3.66SDD52 pKa = 3.77RR53 pKa = 11.84TVPPGTTSPTADD65 pKa = 3.27LGIYY69 pKa = 9.77RR70 pKa = 11.84YY71 pKa = 9.37EE72 pKa = 4.4PPTDD76 pKa = 3.52SGDD79 pKa = 3.58GYY81 pKa = 10.6AQSVSYY87 pKa = 10.78DD88 pKa = 3.17AATDD92 pKa = 3.63TFSVDD97 pKa = 2.87NLAFDD102 pKa = 5.01GANTYY107 pKa = 10.33QRR109 pKa = 11.84DD110 pKa = 3.96DD111 pKa = 3.61MVGSLGPYY119 pKa = 9.52AVYY122 pKa = 10.55EE123 pKa = 4.14GDD125 pKa = 4.08GVFNDD130 pKa = 4.35PVTGVPISQFEE141 pKa = 4.04HH142 pKa = 6.22KK143 pKa = 10.34AIYY146 pKa = 9.81GVSTNTDD153 pKa = 3.18ANGLATTQFAIVRR166 pKa = 11.84TGAYY170 pKa = 8.87VGYY173 pKa = 10.09GFGGFIYY180 pKa = 10.52QRR182 pKa = 11.84DD183 pKa = 3.95GGVTLPTSGQAAYY196 pKa = 9.97SGQYY200 pKa = 9.9AALRR204 pKa = 11.84DD205 pKa = 3.93FNGRR209 pKa = 11.84GGLEE213 pKa = 4.16YY214 pKa = 9.92ATGDD218 pKa = 3.22MTAAIDD224 pKa = 4.36FNDD227 pKa = 4.05FNAGDD232 pKa = 3.85AVQGKK237 pKa = 5.59VTNRR241 pKa = 11.84RR242 pKa = 11.84VYY244 pKa = 10.47DD245 pKa = 3.67VNGSDD250 pKa = 2.86ITASIISALGTQYY263 pKa = 10.9GRR265 pKa = 11.84SYY267 pKa = 10.23TALPQINFVVGPGVLDD283 pKa = 3.59TNGEE287 pKa = 4.11ILGSVSSNVTDD298 pKa = 3.37ATGALQGFEE307 pKa = 3.97TGKK310 pKa = 10.46YY311 pKa = 7.69YY312 pKa = 11.11AVIAGDD318 pKa = 3.6NADD321 pKa = 4.44EE322 pKa = 4.21IVGVLTTEE330 pKa = 4.39ADD332 pKa = 3.54DD333 pKa = 3.88PRR335 pKa = 11.84YY336 pKa = 10.69DD337 pKa = 3.3SVTVRR342 pKa = 11.84EE343 pKa = 4.05TGGFILYY350 pKa = 10.14RR351 pKa = 11.84PP352 pKa = 3.95

Molecular weight: 36.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A095CK48|A0A095CK48_9RHOB Type-4 uracil-DNA glycosylase (Fragment) OS=Rhodovulum sp. NI22 OX=1469613 GN=JT55_18485 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84SRR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.43GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.02GRR39 pKa = 11.84KK40 pKa = 8.91SLSAA44 pKa = 3.86

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3439

0

3439

1042918

40

1915

303.3

32.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.755 ± 0.062

0.862 ± 0.012

5.856 ± 0.031

5.94 ± 0.038

3.801 ± 0.03

8.876 ± 0.033

2.019 ± 0.019

5.116 ± 0.032

3.073 ± 0.033

10.2 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.741 ± 0.021

2.48 ± 0.025

5.209 ± 0.029

3.051 ± 0.021

6.928 ± 0.042

4.888 ± 0.029

5.35 ± 0.025

7.28 ± 0.037

1.351 ± 0.018

2.223 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski