Miniopterus bat coronavirus HKU8

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; Minunacovirus

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B1PHK3|B1PHK3_9ALPC ORF3 protein OS=Miniopterus bat coronavirus HKU8 OX=694001 GN=ORF3 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 10.42SLLVLSLLALLATLSVNAQVTGEE25 pKa = 4.15GGVAEE30 pKa = 4.18GNYY33 pKa = 8.3WVNASCAGWSYY44 pKa = 10.71FYY46 pKa = 11.12ALKK49 pKa = 10.83LGLPPNASAIVTGYY63 pKa = 10.35LPKK66 pKa = 10.29PKK68 pKa = 10.24GWICPRR74 pKa = 11.84FNGAGIYY81 pKa = 8.3TRR83 pKa = 11.84NNANAVFVMYY93 pKa = 8.47RR94 pKa = 11.84TKK96 pKa = 11.11ALAFEE101 pKa = 4.84IGVSSSAGGEE111 pKa = 3.98QYY113 pKa = 10.6SAYY116 pKa = 9.63MAQQNGKK123 pKa = 8.88YY124 pKa = 9.79LVLRR128 pKa = 11.84ICKK131 pKa = 7.98WQNGTLAAPTLQSTSGKK148 pKa = 10.31DD149 pKa = 3.13CIVNVKK155 pKa = 9.65VDD157 pKa = 3.07NHH159 pKa = 5.27MFYY162 pKa = 10.53HH163 pKa = 6.83AAHH166 pKa = 7.47DD167 pKa = 3.76IVGMSWSGDD176 pKa = 3.07AVRR179 pKa = 11.84LYY181 pKa = 9.94TQTDD185 pKa = 3.34TKK187 pKa = 10.59TYY189 pKa = 9.62YY190 pKa = 9.98IPNSWDD196 pKa = 3.26RR197 pKa = 11.84VSIRR201 pKa = 11.84CPDD204 pKa = 3.37KK205 pKa = 10.83FSCSSQIVTKK215 pKa = 10.64AITVNVTTFANGTIDD230 pKa = 5.16KK231 pKa = 10.86YY232 pKa = 11.26AICDD236 pKa = 3.47NCNGYY241 pKa = 8.34PAHH244 pKa = 7.13IFPVSEE250 pKa = 4.43GGLIPADD257 pKa = 3.76FNFSNWFLLTNSSTIVDD274 pKa = 3.49GRR276 pKa = 11.84IVSEE280 pKa = 4.14QPVLLMCLWAVPGLMSTNSFVYY302 pKa = 10.29FNGTAPNKK310 pKa = 9.21QCNGYY315 pKa = 8.38ATDD318 pKa = 3.68SAFEE322 pKa = 3.95ALRR325 pKa = 11.84FSLNFTDD332 pKa = 3.56EE333 pKa = 4.2RR334 pKa = 11.84VFAGSGSVVLLVSGLQYY351 pKa = 11.15KK352 pKa = 8.68FSCTNNSEE360 pKa = 4.04AVIDD364 pKa = 3.77SGIPFGNVVEE374 pKa = 4.3PFYY377 pKa = 11.22CFVSINGTSIFVGMLPAVLRR397 pKa = 11.84EE398 pKa = 4.09IVITRR403 pKa = 11.84YY404 pKa = 10.47GSIYY408 pKa = 10.64LNGFSIFQGPPIQGVLFNVTNRR430 pKa = 11.84GATDD434 pKa = 3.54LWTVALSNFTEE445 pKa = 4.36VLAEE449 pKa = 4.15VQSTAIKK456 pKa = 10.57ALLYY460 pKa = 10.79CDD462 pKa = 5.71DD463 pKa = 5.18PLSQLKK469 pKa = 9.76CQQLQFSLPDD479 pKa = 3.36GFYY482 pKa = 9.99ATASLFQHH490 pKa = 6.22EE491 pKa = 4.7LPRR494 pKa = 11.84TFVTLPRR501 pKa = 11.84HH502 pKa = 5.43FTHH505 pKa = 6.23SWINLRR511 pKa = 11.84IKK513 pKa = 9.01WKK515 pKa = 10.89NGVCYY520 pKa = 9.86NCPPASSWIDD530 pKa = 3.05FVTFNSNGTEE540 pKa = 3.83NVLPEE545 pKa = 3.85RR546 pKa = 11.84TLCVNTTQFTTNLTLIEE563 pKa = 4.21EE564 pKa = 4.58AFSYY568 pKa = 7.75STPVVVRR575 pKa = 11.84ADD577 pKa = 3.48DD578 pKa = 3.99CPFDD582 pKa = 4.04FQSLNNYY589 pKa = 7.66LTFGSICFSLNGTIGKK605 pKa = 9.74GCTLGIYY612 pKa = 9.81KK613 pKa = 10.15RR614 pKa = 11.84ASSQYY619 pKa = 9.3IPIWNVWVAYY629 pKa = 9.37TSGDD633 pKa = 3.44NILGVRR639 pKa = 11.84EE640 pKa = 4.12PNVGVRR646 pKa = 11.84DD647 pKa = 3.71QSVVHH652 pKa = 5.84QNVCTSYY659 pKa = 10.77TIFGHH664 pKa = 5.19SGRR667 pKa = 11.84GIIRR671 pKa = 11.84PANISYY677 pKa = 10.41IAGVYY682 pKa = 7.3YY683 pKa = 9.93TAASGQLLGFKK694 pKa = 9.21NTTTGEE700 pKa = 4.34VFSVTPCNPSQQAVVVKK717 pKa = 10.44DD718 pKa = 3.52RR719 pKa = 11.84LVGVMSSTSTVSIPFNNTIPTPSFYY744 pKa = 10.91YY745 pKa = 10.32HH746 pKa = 6.95SNATSSCDD754 pKa = 3.6DD755 pKa = 3.81PSVVYY760 pKa = 10.1SSIGICDD767 pKa = 3.68DD768 pKa = 3.86GGITFVNSTRR778 pKa = 11.84VRR780 pKa = 11.84GEE782 pKa = 3.65PDD784 pKa = 3.08PAISMGNISVPSNFTVSIQVEE805 pKa = 4.26YY806 pKa = 10.4LQMSIRR812 pKa = 11.84PVSIDD817 pKa = 2.82CAMYY821 pKa = 9.32VCNGNPHH828 pKa = 5.96CTRR831 pKa = 11.84LLQQYY836 pKa = 10.04ISACRR841 pKa = 11.84TIEE844 pKa = 3.89EE845 pKa = 4.26ALQLSARR852 pKa = 11.84LEE854 pKa = 4.25SFEE857 pKa = 4.42VNSMLTVSEE866 pKa = 4.24TALDD870 pKa = 3.76LVNISTFGGDD880 pKa = 3.66YY881 pKa = 10.92NLTALLPQGGGKK893 pKa = 9.86RR894 pKa = 11.84SVIEE898 pKa = 5.43DD899 pKa = 3.28ILFDD903 pKa = 3.79KK904 pKa = 11.1VVTSGLGTVDD914 pKa = 3.79EE915 pKa = 4.9DD916 pKa = 4.87YY917 pKa = 11.2KK918 pKa = 11.11RR919 pKa = 11.84CTNGIGIADD928 pKa = 4.13VPCAQYY934 pKa = 11.47YY935 pKa = 9.06NGIMVLPGVVDD946 pKa = 3.75EE947 pKa = 5.2EE948 pKa = 4.58KK949 pKa = 10.47MSIYY953 pKa = 9.62TASLLGGMTMGGFTPVAALPFALSVQSRR981 pKa = 11.84LNYY984 pKa = 9.76VALQTDD990 pKa = 4.37VLQKK994 pKa = 10.47NQQILANAFNSAIGNITVAFDD1015 pKa = 3.48QVTTAVQQTSDD1026 pKa = 3.63AIKK1029 pKa = 9.55TVASALNKK1037 pKa = 8.89VQSVVNSQGQALHH1050 pKa = 6.21QLTKK1054 pKa = 10.62QLASNFQAISASIEE1068 pKa = 4.23DD1069 pKa = 3.6IYY1071 pKa = 11.82NRR1073 pKa = 11.84LDD1075 pKa = 3.61GLAADD1080 pKa = 4.45ANVDD1084 pKa = 3.63RR1085 pKa = 11.84LITGRR1090 pKa = 11.84LAALNAFVTQTLTKK1104 pKa = 8.05YY1105 pKa = 9.0TEE1107 pKa = 4.09VRR1109 pKa = 11.84ASRR1112 pKa = 11.84LLAQEE1117 pKa = 5.01KK1118 pKa = 10.43INEE1121 pKa = 4.44CVKK1124 pKa = 10.72SQSTRR1129 pKa = 11.84YY1130 pKa = 8.87GFCGNGTHH1138 pKa = 7.47LFSIPNAAPEE1148 pKa = 4.81GIMLFHH1154 pKa = 6.48TVLVPTEE1161 pKa = 4.22YY1162 pKa = 11.1VSVTAWSGYY1171 pKa = 6.77CHH1173 pKa = 6.83NGVGYY1178 pKa = 10.37AVKK1181 pKa = 10.57DD1182 pKa = 3.52VGNSLFQFNNTFYY1195 pKa = 9.59ITPRR1199 pKa = 11.84NMYY1202 pKa = 9.48QPRR1205 pKa = 11.84TPTSADD1211 pKa = 4.29FIRR1214 pKa = 11.84ISGCNVVYY1222 pKa = 10.88VNITDD1227 pKa = 3.56EE1228 pKa = 4.3QLPQVQPEE1236 pKa = 4.49FIDD1239 pKa = 3.57VNKK1242 pKa = 9.54TLEE1245 pKa = 4.2EE1246 pKa = 4.0LMSRR1250 pKa = 11.84LPNNTGPNLPLDD1262 pKa = 4.22IFNQTYY1268 pKa = 10.88LNISAEE1274 pKa = 3.89IDD1276 pKa = 3.29ALEE1279 pKa = 4.32NKK1281 pKa = 10.14SLEE1284 pKa = 4.33LQATADD1290 pKa = 3.78KK1291 pKa = 10.79LQLTIEE1297 pKa = 4.19QLNATLVDD1305 pKa = 4.35LEE1307 pKa = 4.14WLNRR1311 pKa = 11.84FEE1313 pKa = 6.27QYY1315 pKa = 10.86VKK1317 pKa = 9.92WPWWVWLTMIIALVLLTGLMLWCCLATGCCGCCSCMASTLDD1358 pKa = 3.63FRR1360 pKa = 11.84GSRR1363 pKa = 11.84LQQYY1367 pKa = 7.6EE1368 pKa = 4.15VEE1370 pKa = 4.46KK1371 pKa = 11.13VHH1373 pKa = 6.56IQQ1375 pKa = 3.09

Molecular weight: 150.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q0Q099|Q0Q099_9ALPC Nucleoprotein OS=Miniopterus bat coronavirus HKU8 OX=694001 GN=N PE=4 SV=1
MM1 pKa = 7.65FLRR4 pKa = 11.84KK5 pKa = 7.87TRR7 pKa = 11.84TSKK10 pKa = 10.89SSLIRR15 pKa = 11.84LMLIWILLVRR25 pKa = 11.84KK26 pKa = 7.87NQKK29 pKa = 10.19SSVNPRR35 pKa = 11.84KK36 pKa = 9.69RR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.06KK41 pKa = 9.85HH42 pKa = 5.79LLNSTLQHH50 pKa = 6.43LCLHH54 pKa = 6.41HH55 pKa = 6.38QLCCQMLLPMLSLTWWMRR73 pKa = 11.84LLMPIMSLLHH83 pKa = 5.94EE84 pKa = 4.94LSTNKK89 pKa = 9.2MFVLALFAVLAIVNSAPIVNHH110 pKa = 6.35KK111 pKa = 9.91VLNPYY116 pKa = 7.64DD117 pKa = 3.97TPHH120 pKa = 6.51SGFRR124 pKa = 11.84GSCEE128 pKa = 3.71QLALTCRR135 pKa = 11.84LNVQPDD141 pKa = 3.66AFHH144 pKa = 7.11AAATTGSPVQIPRR157 pKa = 11.84ACVSFRR163 pKa = 11.84TVCSAWPKK171 pKa = 10.41YY172 pKa = 10.62DD173 pKa = 3.48FGYY176 pKa = 11.15LMSPDD181 pKa = 3.72VYY183 pKa = 11.28VVTTTPDD190 pKa = 2.67GRR192 pKa = 11.84YY193 pKa = 9.5KK194 pKa = 11.11YY195 pKa = 9.58EE196 pKa = 4.25LNSLAFTPEE205 pKa = 3.87GAQLFYY211 pKa = 10.99TLVDD215 pKa = 4.66GYY217 pKa = 11.22AHH219 pKa = 6.81EE220 pKa = 4.15RR221 pKa = 11.84RR222 pKa = 11.84KK223 pKa = 10.32LIKK226 pKa = 9.59EE227 pKa = 3.95GRR229 pKa = 11.84RR230 pKa = 11.84EE231 pKa = 3.9EE232 pKa = 4.6LPNLDD237 pKa = 3.82AEE239 pKa = 4.78YY240 pKa = 9.81IIHH243 pKa = 6.16PTEE246 pKa = 4.08SQQ248 pKa = 2.91

Molecular weight: 28.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

13719

74

6896

1714.9

189.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.508 ± 0.238

3.419 ± 0.368

5.299 ± 0.375

4.082 ± 0.202

5.882 ± 0.305

6.939 ± 0.354

1.793 ± 0.19

4.578 ± 0.454

5.255 ± 0.521

8.317 ± 0.586

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.012 ± 0.114

5.285 ± 0.312

4.002 ± 0.46

2.784 ± 0.612

3.564 ± 0.361

6.874 ± 0.483

6.218 ± 0.319

10.628 ± 0.711

1.225 ± 0.132

4.337 ± 0.311
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski