Clostridium sp. DSM 8431

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; unclassified Clostridium

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3500 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I7G3L3|A0A1I7G3L3_9CLOT Uncharacterized protein OS=Clostridium sp. DSM 8431 OX=1761781 GN=SAMN04487886_102534 PE=4 SV=1
MM1 pKa = 7.27IAKK4 pKa = 9.48VDD6 pKa = 3.4QDD8 pKa = 3.77TCIGCGLCPSICDD21 pKa = 3.38EE22 pKa = 4.21VFEE25 pKa = 5.39LKK27 pKa = 10.54DD28 pKa = 3.51DD29 pKa = 4.42GKK31 pKa = 11.07SHH33 pKa = 6.71VIVDD37 pKa = 4.47EE38 pKa = 4.28VPEE41 pKa = 4.45DD42 pKa = 3.98CEE44 pKa = 4.41DD45 pKa = 3.54AAKK48 pKa = 10.08EE49 pKa = 4.13AEE51 pKa = 4.38EE52 pKa = 4.35SCPVDD57 pKa = 4.36AIEE60 pKa = 4.98VEE62 pKa = 4.29

Molecular weight: 6.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I7FLA9|A0A1I7FLA9_9CLOT Uncharacterized conserved protein YloU alkaline shock protein (Asp23) family OS=Clostridium sp. DSM 8431 OX=1761781 GN=SAMN04487886_101335 PE=3 SV=1
MM1 pKa = 7.62NEE3 pKa = 3.67ILRR6 pKa = 11.84QIEE9 pKa = 4.01QEE11 pKa = 4.22QIRR14 pKa = 11.84TDD16 pKa = 3.53LPSFNVGDD24 pKa = 3.77NVRR27 pKa = 11.84VHH29 pKa = 5.89VRR31 pKa = 11.84IKK33 pKa = 9.52EE34 pKa = 4.13GNRR37 pKa = 11.84EE38 pKa = 3.61RR39 pKa = 11.84VQAFEE44 pKa = 3.94GTVIKK49 pKa = 10.12KK50 pKa = 10.06QNGGLRR56 pKa = 11.84EE57 pKa = 4.15TFTVRR62 pKa = 11.84RR63 pKa = 11.84VAYY66 pKa = 7.22GTGVEE71 pKa = 4.35RR72 pKa = 11.84TFPLNAPIIEE82 pKa = 4.61KK83 pKa = 10.33IEE85 pKa = 3.68VTRR88 pKa = 11.84RR89 pKa = 11.84GKK91 pKa = 9.47VRR93 pKa = 11.84RR94 pKa = 11.84AKK96 pKa = 10.38LYY98 pKa = 8.55YY99 pKa = 10.46LRR101 pKa = 11.84DD102 pKa = 3.7RR103 pKa = 11.84VGKK106 pKa = 8.84AAKK109 pKa = 10.15VKK111 pKa = 10.33EE112 pKa = 4.06LLRR115 pKa = 5.54

Molecular weight: 13.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3500

0

3500

1035390

25

6057

295.8

33.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.542 ± 0.043

1.276 ± 0.019

5.88 ± 0.039

7.777 ± 0.053

4.389 ± 0.034

6.248 ± 0.043

1.284 ± 0.014

9.545 ± 0.06

9.361 ± 0.045

8.861 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.553 ± 0.019

6.458 ± 0.043

2.535 ± 0.022

2.03 ± 0.022

3.349 ± 0.028

6.631 ± 0.038

4.785 ± 0.036

6.509 ± 0.035

0.663 ± 0.016

4.323 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski