Beihai toti-like virus 4

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KF81|A0A1L3KF81_9VIRU Uncharacterized protein OS=Beihai toti-like virus 4 OX=1922734 PE=4 SV=1
MM1 pKa = 7.43GNAGAKK7 pKa = 10.29GSGEE11 pKa = 4.31GVGSAIGGALTGVSPMLPFPLNLVTGLGGTAANLVGKK48 pKa = 9.91LVGDD52 pKa = 4.32DD53 pKa = 3.34PVVAQEE59 pKa = 4.4AFQQILQQNPQLANQVQVLPPGPQVPPALHH89 pKa = 6.23SQIQTTPSSVVTSGEE104 pKa = 4.0TTPQSNQSGGVEE116 pKa = 3.98GLLGHH121 pKa = 7.27LKK123 pKa = 9.88EE124 pKa = 4.29ANKK127 pKa = 10.14LQSFGPFTHH136 pKa = 7.42APTQLNQQIDD146 pKa = 3.72QLISHH151 pKa = 7.8LGQGHH156 pKa = 5.59LTKK159 pKa = 9.44PTMAQVPTTSQIAPNTSIANKK180 pKa = 10.34LGTTQLTADD189 pKa = 3.87VAEE192 pKa = 4.0QHH194 pKa = 5.26YY195 pKa = 9.99VKK197 pKa = 10.05PIEE200 pKa = 4.26SALVSGEE207 pKa = 3.81AGKK210 pKa = 10.88VGSTNPATNFALALQQGLFPQAQNTMKK237 pKa = 10.28TLEE240 pKa = 4.19NQNQEE245 pKa = 4.25TNIPRR250 pKa = 11.84PLMPGSQNQTEE261 pKa = 4.35LTNPKK266 pKa = 10.16VGTSTMLPLVPVNNARR282 pKa = 11.84QLNLLNGMNTVGGEE296 pKa = 4.03NNIVNSNLGTSTTSARR312 pKa = 11.84PTSFGGILKK321 pKa = 8.87DD322 pKa = 3.48TALDD326 pKa = 3.73VANVGAKK333 pKa = 9.85EE334 pKa = 3.94LGKK337 pKa = 8.49TAGNFLRR344 pKa = 11.84DD345 pKa = 4.0RR346 pKa = 11.84GPDD349 pKa = 3.29MLKK352 pKa = 10.28QGISFLGKK360 pKa = 8.17TLGGLFRR367 pKa = 11.84GKK369 pKa = 10.48LVGDD373 pKa = 4.35APLQPGTWSPGDD385 pKa = 3.88FNLLPNTNEE394 pKa = 3.76MFKK397 pKa = 10.49PGAIGTDD404 pKa = 3.13IVKK407 pKa = 9.87MIGDD411 pKa = 3.96APIDD415 pKa = 3.45GVMSNDD421 pKa = 3.21EE422 pKa = 3.64ASAAVKK428 pKa = 10.81AMTTSIAITLFAKK441 pKa = 10.35LVIIEE446 pKa = 4.23QTLEE450 pKa = 3.73TALPIIFSNNIRR462 pKa = 11.84QAAAQTARR470 pKa = 11.84QITVQNGMVRR480 pKa = 11.84LSQLLFNTDD489 pKa = 4.44LIGNYY494 pKa = 9.32YY495 pKa = 10.01FYY497 pKa = 11.17DD498 pKa = 5.09GIPVYY503 pKa = 9.6RR504 pKa = 11.84TNHH507 pKa = 6.31KK508 pKa = 9.41DD509 pKa = 2.8WHH511 pKa = 6.21VEE513 pKa = 4.01SPIYY517 pKa = 9.97TPTYY521 pKa = 8.55TGLVPISDD529 pKa = 4.32SPPLLGNVTYY539 pKa = 10.31EE540 pKa = 3.88ALKK543 pKa = 10.19PLLSGAPASEE553 pKa = 4.36YY554 pKa = 10.37PSTLRR559 pKa = 11.84IKK561 pKa = 9.04TRR563 pKa = 11.84RR564 pKa = 11.84TDD566 pKa = 2.81IDD568 pKa = 3.5ALLTISSQGTYY579 pKa = 10.36EE580 pKa = 3.99LTASAGYY587 pKa = 10.57SFVCPLVKK595 pKa = 10.64LLGYY599 pKa = 10.2QSAIGQLPGDD609 pKa = 4.18EE610 pKa = 4.23MTQYY614 pKa = 10.46MSGVYY619 pKa = 9.3PVINFSQNPQPGATIFPWRR638 pKa = 11.84SGSSDD643 pKa = 3.42LVNHH647 pKa = 5.93NTWKK651 pKa = 8.09WLKK654 pKa = 9.54VAWVTYY660 pKa = 8.98TEE662 pKa = 4.1FADD665 pKa = 4.86AIVGMRR671 pKa = 11.84APWLDD676 pKa = 3.13VFQPINWGITCAVVFISRR694 pKa = 11.84SEE696 pKa = 4.08MQDD699 pKa = 2.54GATNIVRR706 pKa = 11.84MLSHH710 pKa = 6.77IAYY713 pKa = 8.62PFVIFPRR720 pKa = 11.84EE721 pKa = 4.18VKK723 pKa = 9.07WVQAHH728 pKa = 6.3KK729 pKa = 10.97DD730 pKa = 3.19EE731 pKa = 4.81GFRR734 pKa = 11.84EE735 pKa = 4.16PVMNTTACWPHH746 pKa = 6.95IFSAQIEE753 pKa = 4.77GPDD756 pKa = 3.39QAILFVVTDD765 pKa = 4.27LSDD768 pKa = 3.45RR769 pKa = 11.84QAGFITLGIDD779 pKa = 3.25NMVEE783 pKa = 3.92VLTDD787 pKa = 4.0VITPNTPWQLDD798 pKa = 3.88EE799 pKa = 4.5EE800 pKa = 4.53QHH802 pKa = 4.85EE803 pKa = 4.55TRR805 pKa = 11.84HH806 pKa = 6.26PILMPIFSVINEE818 pKa = 4.06PARR821 pKa = 11.84FQAVIMSEE829 pKa = 3.36IRR831 pKa = 11.84RR832 pKa = 11.84WEE834 pKa = 3.98SYY836 pKa = 10.38YY837 pKa = 11.37GNNSDD842 pKa = 4.57RR843 pKa = 11.84SSAMRR848 pKa = 11.84WCAEE852 pKa = 3.42QSVRR856 pKa = 11.84FGQPKK861 pKa = 9.77NFCFTPTGTHH871 pKa = 6.74NIYY874 pKa = 9.89TGYY877 pKa = 9.18TYY879 pKa = 11.07NADD882 pKa = 3.67PSVSPYY888 pKa = 11.26GDD890 pKa = 3.4LSKK893 pKa = 11.18VFIMSEE899 pKa = 3.99DD900 pKa = 3.4GKK902 pKa = 10.61KK903 pKa = 10.08RR904 pKa = 11.84WMFANMLSTTPLMITPNILDD924 pKa = 4.05GYY926 pKa = 10.6FRR928 pKa = 11.84TPHH931 pKa = 6.05NEE933 pKa = 3.57YY934 pKa = 8.26TTFATTHH941 pKa = 6.27FLGQEE946 pKa = 3.98DD947 pKa = 4.33PVVNFLIEE955 pKa = 4.04RR956 pKa = 11.84GMVSPAEE963 pKa = 4.1EE964 pKa = 4.48YY965 pKa = 7.46PTVRR969 pKa = 11.84FTLAPKK975 pKa = 9.89NYY977 pKa = 10.26SAITEE982 pKa = 4.56LAQLMAFIMDD992 pKa = 3.77SSAQARR998 pKa = 11.84SVTVKK1003 pKa = 10.56DD1004 pKa = 3.79YY1005 pKa = 11.55YY1006 pKa = 11.06SYY1008 pKa = 11.18PNHH1011 pKa = 7.25RR1012 pKa = 11.84LNDD1015 pKa = 3.6DD1016 pKa = 3.51TNQNVVTRR1024 pKa = 11.84MALEE1028 pKa = 4.14KK1029 pKa = 10.75VFFPVGHH1036 pKa = 6.58AVCTNGIQKK1045 pKa = 10.38PSFSVNYY1052 pKa = 9.67QSLEE1056 pKa = 4.07YY1057 pKa = 10.86NHH1059 pKa = 6.75TFPLYY1064 pKa = 10.47FDD1066 pKa = 4.86AVWSFLGDD1074 pKa = 3.37GLTSAKK1080 pKa = 10.26NSTVGVFGRR1089 pKa = 11.84IPWALAEE1096 pKa = 4.07RR1097 pKa = 11.84WHH1099 pKa = 6.7PSFGIRR1105 pKa = 11.84DD1106 pKa = 3.95TNTNVYY1112 pKa = 10.22GLDD1115 pKa = 3.58VKK1117 pKa = 9.97ITEE1120 pKa = 4.24HH1121 pKa = 6.11MGVNGYY1127 pKa = 8.84EE1128 pKa = 3.94PHH1130 pKa = 6.64EE1131 pKa = 5.14LISMMSCDD1139 pKa = 3.81YY1140 pKa = 10.79DD1141 pKa = 3.84VQFIASLFRR1150 pKa = 11.84VSKK1153 pKa = 10.01FLSGLMVSSLQVNNPLWLYY1172 pKa = 10.94DD1173 pKa = 3.15SSTNYY1178 pKa = 9.97FYY1180 pKa = 11.59YY1181 pKa = 10.79LAGATLPLDD1190 pKa = 3.75SVVGRR1195 pKa = 11.84IIALSEE1201 pKa = 3.75AVGVINIPNFNLTPLPICGFVPTYY1225 pKa = 10.24NISGGGRR1232 pKa = 11.84KK1233 pKa = 8.81FRR1235 pKa = 11.84FAAIQPVDD1243 pKa = 3.71SFVNPSGSNDD1253 pKa = 3.31YY1254 pKa = 11.15QLFYY1258 pKa = 10.39PVKK1261 pKa = 8.31TQASLRR1267 pKa = 11.84TTIGYY1272 pKa = 9.55HH1273 pKa = 5.51EE1274 pKa = 5.19FGHH1277 pKa = 6.61PLFSDD1282 pKa = 4.54FTSNNPFAYY1291 pKa = 10.11

Molecular weight:
141.25 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KF81|A0A1L3KF81_9VIRU Uncharacterized protein OS=Beihai toti-like virus 4 OX=1922734 PE=4 SV=1
MM1 pKa = 7.82AEE3 pKa = 3.9NLKK6 pKa = 10.27DD7 pKa = 3.68IKK9 pKa = 10.91VLYY12 pKa = 9.19PDD14 pKa = 3.95YY15 pKa = 11.49EE16 pKa = 4.43MTEE19 pKa = 4.3SMWLDD24 pKa = 3.11IVNIKK29 pKa = 10.14LDD31 pKa = 3.94KK32 pKa = 11.26NDD34 pKa = 3.8FLNDD38 pKa = 3.35LTEE41 pKa = 4.62DD42 pKa = 3.83SNVPGISYY50 pKa = 9.93FSSYY54 pKa = 9.84HH55 pKa = 4.79VNRR58 pKa = 11.84IRR60 pKa = 11.84QMPPMYY66 pKa = 10.44FEE68 pKa = 5.16QEE70 pKa = 4.1FKK72 pKa = 11.01NYY74 pKa = 9.92PLTLKK79 pKa = 10.69FCFSLLLQLHH89 pKa = 6.07PVYY92 pKa = 10.41RR93 pKa = 11.84DD94 pKa = 3.3IIISVLYY101 pKa = 10.26AVNILPNCLTVTKK114 pKa = 10.49YY115 pKa = 10.63IEE117 pKa = 4.16VCKK120 pKa = 10.77KK121 pKa = 8.24LTSWAKK127 pKa = 10.0KK128 pKa = 9.74CNSLASRR135 pKa = 11.84LINNKK140 pKa = 9.09FNCNVEE146 pKa = 4.3DD147 pKa = 4.35KK148 pKa = 10.84FWMILVDD155 pKa = 3.37WDD157 pKa = 3.54IFSGYY162 pKa = 7.79NFKK165 pKa = 11.22YY166 pKa = 10.43NEE168 pKa = 4.13SKK170 pKa = 10.64IIDD173 pKa = 4.21SIKK176 pKa = 9.86EE177 pKa = 3.83WGSGDD182 pKa = 3.08IYY184 pKa = 10.91KK185 pKa = 10.96GNNEE189 pKa = 3.98VSSDD193 pKa = 3.32KK194 pKa = 10.9YY195 pKa = 9.7YY196 pKa = 11.52KK197 pKa = 10.89LMEE200 pKa = 4.66KK201 pKa = 10.58YY202 pKa = 10.15VDD204 pKa = 3.42KK205 pKa = 11.01LLEE208 pKa = 4.48FGTLEE213 pKa = 4.09KK214 pKa = 11.01KK215 pKa = 10.69NILSLRR221 pKa = 11.84DD222 pKa = 3.55WLSDD226 pKa = 3.08PTKK229 pKa = 9.86WVTAGSSNGVRR240 pKa = 11.84VDD242 pKa = 2.96IFSYY246 pKa = 10.84DD247 pKa = 2.92KK248 pKa = 11.11NRR250 pKa = 11.84NVKK253 pKa = 9.69SAATKK258 pKa = 10.07SVFSVKK264 pKa = 10.44YY265 pKa = 10.63DD266 pKa = 3.09VDD268 pKa = 3.88DD269 pKa = 4.4LYY271 pKa = 11.38KK272 pKa = 10.47ILMTNTKK279 pKa = 9.43PQDD282 pKa = 3.2SRR284 pKa = 11.84VNIKK288 pKa = 10.75LEE290 pKa = 4.22AGQKK294 pKa = 9.74NRR296 pKa = 11.84IIVSSGWVNQLRR308 pKa = 11.84MAYY311 pKa = 9.3ISSWLEE317 pKa = 3.51KK318 pKa = 10.44SLKK321 pKa = 9.16NVPWTTLFSTNQDD334 pKa = 2.1AWTRR338 pKa = 11.84NVRR341 pKa = 11.84KK342 pKa = 9.67CSHH345 pKa = 6.47IKK347 pKa = 10.15SGSIMFPLDD356 pKa = 3.87AKK358 pKa = 11.16NFDD361 pKa = 3.73QNVSKK366 pKa = 11.57GEE368 pKa = 3.67IDD370 pKa = 3.47VCFNSIRR377 pKa = 11.84KK378 pKa = 9.15IINKK382 pKa = 9.54SLAGEE387 pKa = 4.11KK388 pKa = 10.5RR389 pKa = 11.84FDD391 pKa = 3.74CLKK394 pKa = 10.9LIDD397 pKa = 4.83IIQNQFWNTFVVYY410 pKa = 10.87KK411 pKa = 9.67NMRR414 pKa = 11.84MLWEE418 pKa = 4.66HH419 pKa = 6.24GMPSGIRR426 pKa = 11.84WTAFLDD432 pKa = 3.66TLINAARR439 pKa = 11.84VCIINDD445 pKa = 3.37ILSKK449 pKa = 11.0GSTIPIKK456 pKa = 10.42EE457 pKa = 3.91YY458 pKa = 9.7TAQGDD463 pKa = 3.86DD464 pKa = 4.69DD465 pKa = 4.7DD466 pKa = 6.89LEE468 pKa = 4.23VSKK471 pKa = 10.67WDD473 pKa = 4.66DD474 pKa = 3.02ILNIFNTYY482 pKa = 9.82NSLGIVLAPQKK493 pKa = 10.87NFVSTKK499 pKa = 5.83MTEE502 pKa = 4.24FLRR505 pKa = 11.84KK506 pKa = 9.83VYY508 pKa = 10.44FSDD511 pKa = 5.82LIMGYY516 pKa = 7.53ITRR519 pKa = 11.84KK520 pKa = 7.68MVNVCFVNPEE530 pKa = 4.15KK531 pKa = 11.19NLVNYY536 pKa = 10.28RR537 pKa = 11.84DD538 pKa = 3.65VDD540 pKa = 3.6LHH542 pKa = 5.94TIRR545 pKa = 11.84DD546 pKa = 3.15IWLEE550 pKa = 3.71LMQRR554 pKa = 11.84GGDD557 pKa = 3.72FEE559 pKa = 4.64YY560 pKa = 11.16CEE562 pKa = 4.44SKK564 pKa = 10.8LIEE567 pKa = 4.98HH568 pKa = 7.09IALVLFNKK576 pKa = 10.07LGKK579 pKa = 9.86IEE581 pKa = 4.94GYY583 pKa = 10.47SKK585 pKa = 10.37LTDD588 pKa = 3.08RR589 pKa = 11.84HH590 pKa = 5.42LAIAKK595 pKa = 9.81DD596 pKa = 3.95VLSTPASLGGLGCLWFGTRR615 pKa = 11.84TWRR618 pKa = 11.84VLQEE622 pKa = 3.99KK623 pKa = 10.13RR624 pKa = 11.84SEE626 pKa = 3.53RR627 pKa = 11.84HH628 pKa = 4.05YY629 pKa = 10.64QLRR632 pKa = 11.84GPLGVWTKK640 pKa = 10.81VLSATNSVTGNVRR653 pKa = 11.84RR654 pKa = 11.84EE655 pKa = 3.77NEE657 pKa = 3.54AVIRR661 pKa = 11.84DD662 pKa = 4.25KK663 pKa = 10.82IVQTLIPKK671 pKa = 9.67HH672 pKa = 5.54EE673 pKa = 4.14RR674 pKa = 11.84VPRR677 pKa = 11.84IIEE680 pKa = 4.27CEE682 pKa = 3.77LLEE685 pKa = 4.52IPFSGLIQVPEE696 pKa = 4.17ITPSLQRR703 pKa = 11.84TLPVWRR709 pKa = 11.84MRR711 pKa = 11.84DD712 pKa = 3.49DD713 pKa = 3.54IGPVDD718 pKa = 4.19VNCVLNACKK727 pKa = 10.25EE728 pKa = 4.02NRR730 pKa = 11.84DD731 pKa = 3.34WGLLRR736 pKa = 11.84SITSEE741 pKa = 4.18SVLGTFDD748 pKa = 4.02NMLSSKK754 pKa = 10.27HH755 pKa = 5.71KK756 pKa = 10.56RR757 pKa = 11.84EE758 pKa = 4.34LLITWVMDD766 pKa = 3.24DD767 pKa = 3.36WTVNAPSHH775 pKa = 5.98RR776 pKa = 11.84VWGKK780 pKa = 7.23TALSAMFVGWKK791 pKa = 9.7NYY793 pKa = 9.94CINRR797 pKa = 11.84WYY799 pKa = 10.12EE800 pKa = 3.88VNNKK804 pKa = 8.83GRR806 pKa = 11.84DD807 pKa = 3.9YY808 pKa = 9.93YY809 pKa = 11.17TKK811 pKa = 10.37LCVSSEE817 pKa = 4.22YY818 pKa = 11.18NFYY821 pKa = 11.02KK822 pKa = 10.58YY823 pKa = 11.18VNTHH827 pKa = 5.02EE828 pKa = 4.95HH829 pKa = 5.53PVVMRR834 pKa = 11.84FF835 pKa = 3.16

Molecular weight:
97.18 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

2126

835

1291

1063.0

119.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.597 ± 1.408

1.176 ± 0.512

4.986 ± 0.811

4.421 ± 0.434

4.563 ± 0.292

6.585 ± 1.283

1.787 ± 0.137

6.397 ± 0.684

5.127 ± 1.797

8.984 ± 0.356

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.681 ± 0.028

6.726 ± 0.345

5.503 ± 1.424

4.045 ± 1.126

3.998 ± 0.686

7.291 ± 0.08

7.008 ± 1.107

7.338 ± 0.195

2.07 ± 0.622

3.716 ± 0.283

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski