Hollyhock yellow vein mosaic Islamabad virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Hollyhock yellow vein mosaic virus

Average proteome isoelectric point is 7.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0G4DIL0|A0A0G4DIL0_9GEMI Replication enhancer OS=Hollyhock yellow vein mosaic Islamabad virus OX=1504732 GN=REn PE=3 SV=1
MM1 pKa = 7.42PRR3 pKa = 11.84AFKK6 pKa = 10.99LNAKK10 pKa = 9.86NYY12 pKa = 8.38FLTYY16 pKa = 9.06PKK18 pKa = 10.53CSLTKK23 pKa = 10.72EE24 pKa = 4.17EE25 pKa = 4.86TLSQLQNLHH34 pKa = 5.69TPVNKK39 pKa = 10.11KK40 pKa = 9.89YY41 pKa = 10.82IKK43 pKa = 9.68ICRR46 pKa = 11.84EE47 pKa = 3.53LHH49 pKa = 6.26EE50 pKa = 4.93NGEE53 pKa = 4.28PHH55 pKa = 6.66LHH57 pKa = 6.22VLIQFDD63 pKa = 4.6GKK65 pKa = 8.92YY66 pKa = 9.41QCTNKK71 pKa = 10.06RR72 pKa = 11.84FFDD75 pKa = 3.89LVSPTRR81 pKa = 11.84SAHH84 pKa = 4.86FHH86 pKa = 6.55PNIQGAKK93 pKa = 9.25SSSDD97 pKa = 3.11VKK99 pKa = 11.17SYY101 pKa = 11.26LEE103 pKa = 4.24KK104 pKa = 11.19DD105 pKa = 3.24GDD107 pKa = 3.91TLDD110 pKa = 3.28WGEE113 pKa = 3.86FQIDD117 pKa = 3.21GRR119 pKa = 11.84SARR122 pKa = 11.84GGQQSANDD130 pKa = 3.91AYY132 pKa = 10.27ATALNAGSKK141 pKa = 10.23SEE143 pKa = 3.84ALRR146 pKa = 11.84VIKK149 pKa = 10.21EE150 pKa = 4.18LAPKK154 pKa = 10.3DD155 pKa = 3.74YY156 pKa = 11.2VLQFHH161 pKa = 6.76NLNANLDD168 pKa = 4.31RR169 pKa = 11.84IFTPPMEE176 pKa = 4.56VYY178 pKa = 10.67VSPFSSSSFDD188 pKa = 3.41QVPEE192 pKa = 3.98EE193 pKa = 4.13LEE195 pKa = 3.58EE196 pKa = 3.9WAAEE200 pKa = 4.15NVVSAAARR208 pKa = 11.84PLRR211 pKa = 11.84PISIVIEE218 pKa = 4.23GDD220 pKa = 3.2SRR222 pKa = 11.84TGKK225 pKa = 8.55TMWARR230 pKa = 11.84ALGPHH235 pKa = 6.7NYY237 pKa = 10.19LCGHH241 pKa = 7.38LDD243 pKa = 4.18LSPKK247 pKa = 10.02VYY249 pKa = 10.89SNEE252 pKa = 3.02AWYY255 pKa = 10.82NVIDD259 pKa = 5.29DD260 pKa = 4.41VDD262 pKa = 3.61PHH264 pKa = 5.85YY265 pKa = 11.0LKK267 pKa = 10.7HH268 pKa = 6.2FKK270 pKa = 10.7EE271 pKa = 4.46FMGAQRR277 pKa = 11.84DD278 pKa = 3.81WQSNTKK284 pKa = 9.6YY285 pKa = 10.57GKK287 pKa = 9.12PVQIKK292 pKa = 10.41GGIPTIFLCNPGPNSSYY309 pKa = 11.34KK310 pKa = 10.62EE311 pKa = 3.85FLDD314 pKa = 3.79EE315 pKa = 4.63EE316 pKa = 4.37KK317 pKa = 11.05NAALKK322 pKa = 10.45NWALKK327 pKa = 10.17NATFITLEE335 pKa = 4.26GPLYY339 pKa = 10.84SGSNQSAAQASQEE352 pKa = 3.81GDD354 pKa = 3.32QASTCC359 pKa = 3.84

Molecular weight:
40.3 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0G4DI24|A0A0G4DI24_9GEMI Protein V2 OS=Hollyhock yellow vein mosaic Islamabad virus OX=1504732 GN=V2 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.35RR4 pKa = 11.84PADD7 pKa = 3.87IIISTPASKK16 pKa = 10.3VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 2.99SPYY27 pKa = 10.45ASRR30 pKa = 11.84AAVPIVRR37 pKa = 11.84VTKK40 pKa = 10.49ARR42 pKa = 11.84AWANRR47 pKa = 11.84PMNRR51 pKa = 11.84KK52 pKa = 7.87PRR54 pKa = 11.84MYY56 pKa = 10.68RR57 pKa = 11.84MYY59 pKa = 10.44RR60 pKa = 11.84SPDD63 pKa = 3.21VPRR66 pKa = 11.84GCEE69 pKa = 4.34GPCKK73 pKa = 10.01VQSFEE78 pKa = 4.1SRR80 pKa = 11.84HH81 pKa = 6.13DD82 pKa = 3.26IQHH85 pKa = 6.35IGKK88 pKa = 8.97VMCVSDD94 pKa = 3.5VTRR97 pKa = 11.84GIGLTHH103 pKa = 6.67RR104 pKa = 11.84VGKK107 pKa = 9.66RR108 pKa = 11.84FCVKK112 pKa = 9.94SVYY115 pKa = 10.52VLGKK119 pKa = 9.41IWMDD123 pKa = 3.35EE124 pKa = 4.04NIKK127 pKa = 9.32TKK129 pKa = 10.6NHH131 pKa = 5.78TNSVMFFLVRR141 pKa = 11.84DD142 pKa = 3.84RR143 pKa = 11.84RR144 pKa = 11.84PVDD147 pKa = 3.13KK148 pKa = 10.28PQDD151 pKa = 3.56FGEE154 pKa = 4.33VFNMFDD160 pKa = 4.29NEE162 pKa = 4.11PSTATVKK169 pKa = 10.38NVHH172 pKa = 6.5RR173 pKa = 11.84DD174 pKa = 3.15RR175 pKa = 11.84YY176 pKa = 8.56QVLRR180 pKa = 11.84KK181 pKa = 8.38WHH183 pKa = 5.41STVTGGLYY191 pKa = 10.35ASKK194 pKa = 10.33EE195 pKa = 3.73QALVKK200 pKa = 10.65KK201 pKa = 9.55FIRR204 pKa = 11.84VNNHH208 pKa = 3.56VVYY211 pKa = 10.34NQQEE215 pKa = 3.83AGKK218 pKa = 10.1YY219 pKa = 8.23EE220 pKa = 4.02NHH222 pKa = 6.47TEE224 pKa = 3.99NALMLYY230 pKa = 7.52MACTHH235 pKa = 7.06ASNPVYY241 pKa = 9.86ATLKK245 pKa = 9.47IRR247 pKa = 11.84IYY249 pKa = 10.67FYY251 pKa = 11.26DD252 pKa = 3.51SVSNN256 pKa = 3.9

Molecular weight:
29.66 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1104

100

359

184.0

21.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.25 ± 0.757

2.174 ± 0.536

4.801 ± 0.333

4.982 ± 0.732

4.167 ± 0.347

5.072 ± 0.35

4.076 ± 0.675

4.71 ± 0.698

5.707 ± 0.747

6.884 ± 0.865

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.536 ± 0.574

5.616 ± 0.641

5.978 ± 0.58

4.801 ± 0.481

6.975 ± 1.307

8.243 ± 1.318

5.254 ± 0.545

6.612 ± 1.285

1.359 ± 0.234

3.804 ± 0.394

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski