Halalkalibacillus sediminis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Halalkalibacillus

Average proteome isoelectric point is 5.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2899 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I0QSI5|A0A2I0QSI5_9BACI Transcriptional regulator OS=Halalkalibacillus sediminis OX=2018042 GN=CEY16_11145 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.49KK3 pKa = 10.39NIAVLFTAILLIGLLSACGVLGGGSDD29 pKa = 3.84SDD31 pKa = 4.25SEE33 pKa = 4.65SGGTVTIGGKK43 pKa = 8.94PWTEE47 pKa = 3.93QYY49 pKa = 10.58ILPYY53 pKa = 10.06IIGIHH58 pKa = 5.91LEE60 pKa = 4.14EE61 pKa = 4.37NTDD64 pKa = 3.84FDD66 pKa = 3.81VQYY69 pKa = 11.07EE70 pKa = 4.18EE71 pKa = 4.91GLGEE75 pKa = 4.27VAILTPAIDD84 pKa = 4.93DD85 pKa = 3.88GDD87 pKa = 3.5IDD89 pKa = 4.57VYY91 pKa = 11.33VEE93 pKa = 3.93YY94 pKa = 10.32TGTGLEE100 pKa = 4.12AVLNEE105 pKa = 3.92QAEE108 pKa = 4.55EE109 pKa = 4.55GEE111 pKa = 4.74SADD114 pKa = 3.95SVYY117 pKa = 11.35DD118 pKa = 3.7RR119 pKa = 11.84VKK121 pKa = 10.59EE122 pKa = 4.31GYY124 pKa = 9.65EE125 pKa = 3.78EE126 pKa = 4.18EE127 pKa = 5.59FNVTWLEE134 pKa = 3.74PLGFEE139 pKa = 4.11NTYY142 pKa = 9.21TLAYY146 pKa = 9.79HH147 pKa = 7.15PDD149 pKa = 3.33SGYY152 pKa = 11.13DD153 pKa = 3.22ADD155 pKa = 4.26TYY157 pKa = 11.57SDD159 pKa = 3.69LVEE162 pKa = 4.07TSKK165 pKa = 11.43NEE167 pKa = 3.77DD168 pKa = 3.6MIFGAPHH175 pKa = 6.78AFYY178 pKa = 10.59EE179 pKa = 4.54RR180 pKa = 11.84EE181 pKa = 3.88GDD183 pKa = 3.81GYY185 pKa = 11.29DD186 pKa = 4.18AMLEE190 pKa = 4.12VYY192 pKa = 9.81PFEE195 pKa = 4.16FTEE198 pKa = 4.52TEE200 pKa = 4.13SLDD203 pKa = 3.66ANVMYY208 pKa = 9.9EE209 pKa = 3.83ALEE212 pKa = 4.13SQEE215 pKa = 4.01VDD217 pKa = 4.95LIPAFTTDD225 pKa = 3.92GRR227 pKa = 11.84IEE229 pKa = 4.27RR230 pKa = 11.84YY231 pKa = 9.61NLEE234 pKa = 4.18TTEE237 pKa = 4.6DD238 pKa = 3.66DD239 pKa = 3.33QGFFPKK245 pKa = 10.27YY246 pKa = 9.91DD247 pKa = 3.39AVPLVRR253 pKa = 11.84QEE255 pKa = 4.9ALDD258 pKa = 3.86EE259 pKa = 4.35NPGLKK264 pKa = 9.86EE265 pKa = 4.94AINEE269 pKa = 4.0LAGQISEE276 pKa = 5.02EE277 pKa = 4.19EE278 pKa = 4.07MQEE281 pKa = 3.49MNARR285 pKa = 11.84VDD287 pKa = 3.12IDD289 pKa = 3.81GDD291 pKa = 3.59EE292 pKa = 4.48HH293 pKa = 4.91QTVARR298 pKa = 11.84EE299 pKa = 3.77FLKK302 pKa = 10.84EE303 pKa = 3.83KK304 pKa = 10.83GIIEE308 pKa = 4.14

Molecular weight: 34.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I0QT15|A0A2I0QT15_9BACI DNA replication initiation control protein YabA OS=Halalkalibacillus sediminis OX=2018042 GN=CEY16_12215 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.35QPNNRR10 pKa = 11.84KK11 pKa = 9.28RR12 pKa = 11.84SKK14 pKa = 9.57VHH16 pKa = 5.77GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.19NGRR28 pKa = 11.84KK29 pKa = 8.49VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.37GRR39 pKa = 11.84KK40 pKa = 6.43TLSAA44 pKa = 4.15

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2899

0

2899

832733

26

2872

287.2

32.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.318 ± 0.045

0.587 ± 0.013

5.817 ± 0.042

8.419 ± 0.062

4.578 ± 0.043

6.754 ± 0.044

2.131 ± 0.026

7.7 ± 0.053

6.425 ± 0.047

9.402 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.87 ± 0.023

4.512 ± 0.035

3.478 ± 0.027

3.946 ± 0.033

4.031 ± 0.035

6.226 ± 0.033

5.184 ± 0.037

7.086 ± 0.033

1.003 ± 0.019

3.535 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski