Lactobacillus phage Lv-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B8R670|B8R670_9CAUD Integrase OS=Lactobacillus phage Lv-1 OX=578234 PE=3 SV=1
MM1 pKa = 7.7KK2 pKa = 10.39ANQILEE8 pKa = 3.99LSKK11 pKa = 11.22NEE13 pKa = 4.73KK14 pKa = 9.54IDD16 pKa = 2.96IYY18 pKa = 11.28NYY20 pKa = 8.75YY21 pKa = 10.73DD22 pKa = 3.28EE23 pKa = 5.57GKK25 pKa = 9.94GINVSTFNTIDD36 pKa = 4.48AINDD40 pKa = 3.74LASLKK45 pKa = 10.48VKK47 pKa = 10.65YY48 pKa = 9.41SEE50 pKa = 4.54RR51 pKa = 11.84DD52 pKa = 2.78QDD54 pKa = 3.49AYY56 pKa = 11.41LDD58 pKa = 4.03EE59 pKa = 4.55EE60 pKa = 4.62VEE62 pKa = 4.73AIYY65 pKa = 10.95EE66 pKa = 4.25DD67 pKa = 4.15FDD69 pKa = 4.31YY70 pKa = 11.28LNKK73 pKa = 10.77AIDD76 pKa = 3.37IDD78 pKa = 4.18EE79 pKa = 4.52DD80 pKa = 3.91SKK82 pKa = 11.89YY83 pKa = 11.23SDD85 pKa = 3.45VVKK88 pKa = 9.44QTVDD92 pKa = 3.41AIDD95 pKa = 3.54YY96 pKa = 7.54FKK98 pKa = 11.29NFEE101 pKa = 4.44DD102 pKa = 4.37PVVVDD107 pKa = 3.33YY108 pKa = 11.72NKK110 pKa = 10.97ASFEE114 pKa = 4.32LDD116 pKa = 2.79INSYY120 pKa = 11.06FEE122 pKa = 4.37LTDD125 pKa = 3.7FTSFEE130 pKa = 3.98QLADD134 pKa = 3.82GDD136 pKa = 4.58SKK138 pKa = 11.18LVSAIHH144 pKa = 6.71RR145 pKa = 11.84LQSDD149 pKa = 3.5IEE151 pKa = 4.18DD152 pKa = 3.9AEE154 pKa = 4.16

Molecular weight: 17.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B8R693|B8R693_9CAUD DNA methylase OS=Lactobacillus phage Lv-1 OX=578234 PE=4 SV=1
MM1 pKa = 7.27TRR3 pKa = 11.84LTAQQMHH10 pKa = 6.86ILKK13 pKa = 10.41ASKK16 pKa = 10.14LRR18 pKa = 11.84NNQSTKK24 pKa = 8.0QQNWTCLTFSQNTKK38 pKa = 9.47NKK40 pKa = 8.63KK41 pKa = 6.5TEE43 pKa = 3.83KK44 pKa = 10.04DD45 pKa = 3.25RR46 pKa = 11.84KK47 pKa = 8.69KK48 pKa = 9.31TGSQAEE54 pKa = 4.88GYY56 pKa = 10.6KK57 pKa = 10.64LILVVV62 pKa = 4.14

Molecular weight: 7.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47

0

47

11495

62

1560

244.6

27.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.281 ± 0.523

0.792 ± 0.146

6.646 ± 0.445

5.698 ± 0.318

4.08 ± 0.345

6.246 ± 0.602

1.688 ± 0.141

6.707 ± 0.236

8.882 ± 0.524

7.925 ± 0.276

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.157 ± 0.129

5.968 ± 0.318

2.793 ± 0.234

4.872 ± 0.277

3.61 ± 0.263

7.012 ± 0.4

6.446 ± 0.333

5.985 ± 0.275

1.548 ± 0.161

3.662 ± 0.329
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski