Rhodococcus rhodnii LMG 5362

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Rhodococcus; Rhodococcus rhodnii

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4412 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7WI45|R7WI45_9NOCA YcaO domain-containing protein OS=Rhodococcus rhodnii LMG 5362 OX=1273125 GN=Rrhod_3826 PE=4 SV=1
MM1 pKa = 7.52ALAATTGLVLTACSGEE17 pKa = 4.27DD18 pKa = 3.14AATASSGYY26 pKa = 9.81DD27 pKa = 3.55GPIGAIDD34 pKa = 4.77LSQDD38 pKa = 3.27CPATVVIQTDD48 pKa = 3.38WNPEE52 pKa = 3.89VEE54 pKa = 4.69HH55 pKa = 6.35GGSYY59 pKa = 10.88QMLGPDD65 pKa = 3.74PQIDD69 pKa = 3.86ANSKK73 pKa = 8.36RR74 pKa = 11.84TSGPLFASGEE84 pKa = 4.22YY85 pKa = 9.85TGVDD89 pKa = 3.31LEE91 pKa = 4.39IRR93 pKa = 11.84SGGPAIGFQSVTSQMYY109 pKa = 9.35QDD111 pKa = 4.1PDD113 pKa = 3.08IMLGYY118 pKa = 10.37VDD120 pKa = 4.09TDD122 pKa = 3.49QAISNSGNNPTIGVYY137 pKa = 9.93AALEE141 pKa = 4.17KK142 pKa = 10.96SPMMLMWDD150 pKa = 3.7PEE152 pKa = 4.41TYY154 pKa = 10.31PDD156 pKa = 3.57VEE158 pKa = 4.67RR159 pKa = 11.84IEE161 pKa = 4.63DD162 pKa = 4.41LKK164 pKa = 10.92DD165 pKa = 3.33TPAKK169 pKa = 9.91VLYY172 pKa = 10.33FEE174 pKa = 5.42GEE176 pKa = 3.75AYY178 pKa = 9.52MDD180 pKa = 4.09YY181 pKa = 10.23LTGSGVLNPDD191 pKa = 3.47QVDD194 pKa = 3.61GSYY197 pKa = 11.23DD198 pKa = 3.36GTPANFVAAGGANGQQGFASSEE220 pKa = 4.05PYY222 pKa = 10.01VYY224 pKa = 10.6EE225 pKa = 4.48NEE227 pKa = 4.08VEE229 pKa = 4.37GWKK232 pKa = 10.62KK233 pKa = 9.83PVNFEE238 pKa = 4.92LIHH241 pKa = 5.89DD242 pKa = 4.36TGYY245 pKa = 8.57PTYY248 pKa = 10.33KK249 pKa = 10.45SPLAVRR255 pKa = 11.84ADD257 pKa = 3.46ALEE260 pKa = 4.39EE261 pKa = 3.88NSACLEE267 pKa = 3.95KK268 pKa = 10.59LVPVMQQASVDD279 pKa = 3.96YY280 pKa = 10.23FADD283 pKa = 3.86PAAANAVILDD293 pKa = 3.58AVEE296 pKa = 4.04QYY298 pKa = 8.91NTGWVYY304 pKa = 10.79GQGNADD310 pKa = 3.7YY311 pKa = 10.51GLEE314 pKa = 4.05TMTEE318 pKa = 4.13LEE320 pKa = 4.18LVGNGTTPTHH330 pKa = 6.51GDD332 pKa = 3.01FDD334 pKa = 4.15EE335 pKa = 5.78ARR337 pKa = 11.84VQQLIDD343 pKa = 2.94ITRR346 pKa = 11.84PVFNEE351 pKa = 3.63QGKK354 pKa = 9.66DD355 pKa = 3.6VPADD359 pKa = 3.9LAPDD363 pKa = 3.4AVVTNRR369 pKa = 11.84FIDD372 pKa = 3.8EE373 pKa = 4.75SISFPP378 pKa = 4.22

Molecular weight: 40.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7WK57|R7WK57_9NOCA Uncharacterized protein OS=Rhodococcus rhodnii LMG 5362 OX=1273125 GN=Rrhod_2940 PE=4 SV=1
MM1 pKa = 6.73FTVAKK6 pKa = 9.94GKK8 pKa = 8.69RR9 pKa = 11.84TFQPNNRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84ARR20 pKa = 11.84VHH22 pKa = 5.99GFRR25 pKa = 11.84LRR27 pKa = 11.84MRR29 pKa = 11.84TRR31 pKa = 11.84AGRR34 pKa = 11.84AIVTARR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84KK43 pKa = 9.64GRR45 pKa = 11.84ASLTAA50 pKa = 4.1

Molecular weight: 5.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4412

0

4412

1323253

30

11261

299.9

32.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.45 ± 0.053

0.713 ± 0.01

6.725 ± 0.035

5.57 ± 0.031

2.916 ± 0.022

9.175 ± 0.033

2.169 ± 0.019

3.832 ± 0.025

1.676 ± 0.029

9.608 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.866 ± 0.015

1.735 ± 0.018

5.724 ± 0.032

2.434 ± 0.021

7.899 ± 0.044

5.499 ± 0.027

6.31 ± 0.027

9.34 ± 0.041

1.437 ± 0.016

1.923 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski