Sphingobium quisquiliarum P25

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingobium; Sphingobium quisquiliarum

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3949 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T0H161|T0H161_9SPHN Replicative DNA helicase OS=Sphingobium quisquiliarum P25 OX=1329909 GN=L288_04615 PE=3 SV=1
MM1 pKa = 6.79HH2 pKa = 6.74TTYY5 pKa = 11.24GSATVRR11 pKa = 11.84LYY13 pKa = 10.81HH14 pKa = 7.17LSDD17 pKa = 3.54AQEE20 pKa = 4.34GGAATTLFYY29 pKa = 11.3GSLDD33 pKa = 3.27EE34 pKa = 4.67AMRR37 pKa = 11.84IAAQQPEE44 pKa = 4.45DD45 pKa = 3.78VQDD48 pKa = 4.0GLFLATDD55 pKa = 3.75NDD57 pKa = 3.99VIAYY61 pKa = 10.0LDD63 pKa = 4.6LIGDD67 pKa = 4.24

Molecular weight: 7.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T0GTU2|T0GTU2_9SPHN RNA 2' 3'-cyclic phosphodiesterase OS=Sphingobium quisquiliarum P25 OX=1329909 GN=L288_09630 PE=3 SV=1
MM1 pKa = 7.77LIGYY5 pKa = 9.63ARR7 pKa = 11.84VSKK10 pKa = 11.07ADD12 pKa = 3.59GSQSLDD18 pKa = 3.58LQHH21 pKa = 7.35DD22 pKa = 4.19ALRR25 pKa = 11.84AAGVEE30 pKa = 4.01PGNIYY35 pKa = 10.38DD36 pKa = 4.43DD37 pKa = 3.95RR38 pKa = 11.84ASGSRR43 pKa = 11.84DD44 pKa = 3.48DD45 pKa = 4.67RR46 pKa = 11.84PGLAACLKK54 pKa = 9.81SLRR57 pKa = 11.84DD58 pKa = 3.55GDD60 pKa = 4.36VLIVWKK66 pKa = 10.36LDD68 pKa = 3.35RR69 pKa = 11.84LGRR72 pKa = 11.84TLTHH76 pKa = 6.69LVSTVQNLSDD86 pKa = 3.51RR87 pKa = 11.84GIGLRR92 pKa = 11.84VLTGKK97 pKa = 10.03GAQIDD102 pKa = 3.93TTTPSGRR109 pKa = 11.84MVFGIFAALLQRR121 pKa = 11.84LAAVRR126 pKa = 11.84GRR128 pKa = 11.84LSLCADD134 pKa = 3.29QAAAAKK140 pKa = 8.3WWLSMPMASVSAEE153 pKa = 3.67GPMPKK158 pKa = 10.15ALSTRR163 pKa = 11.84RR164 pKa = 11.84TSPLMPGCRR173 pKa = 11.84HH174 pKa = 5.9QGRR177 pKa = 11.84ACPLRR182 pKa = 11.84RR183 pKa = 11.84ARR185 pKa = 11.84MTSNPLIVAA194 pKa = 4.66

Molecular weight: 20.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3949

0

3949

1198606

29

1618

303.5

32.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.065 ± 0.057

0.8 ± 0.01

5.89 ± 0.03

5.472 ± 0.038

3.53 ± 0.022

8.844 ± 0.036

2.086 ± 0.018

5.156 ± 0.026

3.023 ± 0.03

10.03 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.619 ± 0.02

2.51 ± 0.026

5.369 ± 0.029

3.296 ± 0.022

7.516 ± 0.04

5.365 ± 0.026

4.856 ± 0.024

6.894 ± 0.03

1.432 ± 0.016

2.247 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski