Halomonas gudaonensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Halomonas

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3869 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G8MXX2|A0A1G8MXX2_9GAMM Putative NAD(P)H nitroreductase OS=Halomonas gudaonensis OX=376427 GN=SAMN04487954_101158 PE=3 SV=1
MM1 pKa = 7.11IRR3 pKa = 11.84HH4 pKa = 5.31MAVAVFLGLLSLPAMADD21 pKa = 3.46DD22 pKa = 4.43CSVTVEE28 pKa = 5.42ANDD31 pKa = 3.6QMQFDD36 pKa = 4.04TDD38 pKa = 4.26AIEE41 pKa = 4.36VSQSCDD47 pKa = 2.82EE48 pKa = 4.08FTVNLEE54 pKa = 4.34HH55 pKa = 6.52TGSMPVEE62 pKa = 4.28SMGHH66 pKa = 4.51NWVLTRR72 pKa = 11.84EE73 pKa = 4.23DD74 pKa = 4.26DD75 pKa = 3.68MSDD78 pKa = 3.25VAGAGAGAGVDD89 pKa = 3.23NDD91 pKa = 4.19YY92 pKa = 11.16IEE94 pKa = 5.26PGDD97 pKa = 3.97DD98 pKa = 2.56RR99 pKa = 11.84VIAHH103 pKa = 6.4TEE105 pKa = 4.15LIGGGEE111 pKa = 4.27STSVTFAVDD120 pKa = 3.42EE121 pKa = 4.8LEE123 pKa = 5.11DD124 pKa = 4.94GEE126 pKa = 5.92DD127 pKa = 3.46YY128 pKa = 11.25TFFCSFPGHH137 pKa = 6.4SGPMQGSVSLVDD149 pKa = 3.45

Molecular weight: 15.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G8PL96|A0A1G8PL96_9GAMM 5-formyltetrahydrofolate cyclo-ligase OS=Halomonas gudaonensis OX=376427 GN=SAMN04487954_102116 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.34RR12 pKa = 11.84KK13 pKa = 9.1RR14 pKa = 11.84VHH16 pKa = 6.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.3NGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3869

0

3869

1252047

39

8874

323.6

35.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.665 ± 0.055

0.912 ± 0.013

5.863 ± 0.052

6.786 ± 0.042

3.361 ± 0.028

8.331 ± 0.051

2.47 ± 0.021

4.39 ± 0.034

2.361 ± 0.037

11.576 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.399 ± 0.022

2.315 ± 0.023

4.979 ± 0.031

3.569 ± 0.025

7.751 ± 0.049

5.24 ± 0.035

4.947 ± 0.026

7.35 ± 0.034

1.486 ± 0.02

2.251 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski