Nocardioides sp. MAH-18

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides; unclassified Nocardioides

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4572 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6L6XT46|A0A6L6XT46_9ACTN DNA-3-methyladenine glycosylase I OS=Nocardioides sp. MAH-18 OX=2682843 GN=GON03_15295 PE=4 SV=1
MM1 pKa = 7.72HH2 pKa = 7.69DD3 pKa = 3.2VDD5 pKa = 4.98GSVSLTFYY13 pKa = 10.7CGEE16 pKa = 4.41LPADD20 pKa = 4.08SAIHH24 pKa = 6.16AAGHH28 pKa = 5.75EE29 pKa = 4.18PNGYY33 pKa = 8.68FWEE36 pKa = 4.66GLVQYY41 pKa = 8.23LAPDD45 pKa = 3.13VADD48 pKa = 3.76RR49 pKa = 11.84VEE51 pKa = 4.45LDD53 pKa = 3.4SEE55 pKa = 4.48AGMFAAYY62 pKa = 10.13GDD64 pKa = 3.89RR65 pKa = 11.84STLEE69 pKa = 3.92RR70 pKa = 11.84LQTLIDD76 pKa = 4.53PYY78 pKa = 11.16LDD80 pKa = 3.89DD81 pKa = 4.96AEE83 pKa = 4.36QVAVAIRR90 pKa = 11.84EE91 pKa = 4.3AEE93 pKa = 4.03GSGFSFDD100 pKa = 4.55DD101 pKa = 3.5

Molecular weight: 10.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6L6XSX4|A0A6L6XSX4_9ACTN Na+/H+ antiporter OS=Nocardioides sp. MAH-18 OX=2682843 GN=GON03_14940 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.24TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.07LGKK33 pKa = 9.87

Molecular weight: 4.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4572

0

4572

1479584

30

3341

323.6

34.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.345 ± 0.052

0.723 ± 0.011

6.579 ± 0.031

5.681 ± 0.038

2.781 ± 0.018

9.228 ± 0.035

2.181 ± 0.018

3.425 ± 0.02

1.831 ± 0.025

10.223 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.803 ± 0.014

1.716 ± 0.019

5.653 ± 0.027

2.733 ± 0.017

7.629 ± 0.041

5.118 ± 0.023

6.138 ± 0.039

9.641 ± 0.03

1.566 ± 0.015

2.005 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski