Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Bradyrhizobiaceae; Bradyrhizobium; Bradyrhizobium diazoefficiens

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8253 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q89DD5|Q89DD5_BRADU Bsl7504 protein OS=Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) OX=224911 GN=bsl7504 PE=4 SV=1
MM1 pKa = 7.13TVIPPASGMTLHH13 pKa = 7.4DD14 pKa = 5.02GDD16 pKa = 4.31TMDD19 pKa = 3.14VSGGISVDD27 pKa = 3.1STTILAGALQTVGGSFATSTATHH50 pKa = 5.34TVINGGEE57 pKa = 3.89QDD59 pKa = 3.86VVFDD63 pKa = 4.34GVASYY68 pKa = 7.43TTINAGGVQRR78 pKa = 11.84VTGEE82 pKa = 4.24YY83 pKa = 9.0IHH85 pKa = 6.75EE86 pKa = 4.79GPTPGEE92 pKa = 4.01VDD94 pKa = 2.97HH95 pKa = 6.07TTINGGGEE103 pKa = 3.94QDD105 pKa = 3.58VDD107 pKa = 3.47TGIATTTTVNSGGVQNVTNGGSASGTIVNSGGVLRR142 pKa = 11.84VSGEE146 pKa = 4.32TVEE149 pKa = 4.31TAPVYY154 pKa = 9.49PEE156 pKa = 3.57PVIRR160 pKa = 11.84SVATAATINYY170 pKa = 9.27QGEE173 pKa = 4.41LDD175 pKa = 3.77VTGGGTAVNAIVNGGVMTVSGSIPDD200 pKa = 3.87PFANVPGQPAVDD212 pKa = 3.35ASMATGTIVEE222 pKa = 4.55GFGTLTVSDD231 pKa = 4.22GALVSGTILQNFGTLNVDD249 pKa = 3.41AGGTATGTTINYY261 pKa = 7.19GTMVLSAGATAINTTVNQYY280 pKa = 10.75GGLVVLGNADD290 pKa = 3.38FSGTVFNAGSTLQIGDD306 pKa = 3.62GTIEE310 pKa = 4.19NGFTVPKK317 pKa = 10.24SVTLAVLDD325 pKa = 4.19GGVLSNSVISAGATLIAEE343 pKa = 4.92AGATLSNTTFQSGATFVADD362 pKa = 3.43YY363 pKa = 10.25GYY365 pKa = 9.08TEE367 pKa = 4.15NGFTVSDD374 pKa = 4.77GILFKK379 pKa = 11.43VFGTATNTTISAGGKK394 pKa = 9.61QILGNYY400 pKa = 9.32DD401 pKa = 3.63LGATASNTVINGGEE415 pKa = 3.91QDD417 pKa = 3.56VGLASTASYY426 pKa = 7.77TTINTGGIQRR436 pKa = 11.84VAGIYY441 pKa = 10.29LHH443 pKa = 7.18DD444 pKa = 4.5APGPGRR450 pKa = 11.84ADD452 pKa = 2.85HH453 pKa = 5.78TTVNNGAEE461 pKa = 4.13QDD463 pKa = 3.42VDD465 pKa = 3.67TGIVTSTTVNGGGVQNLTNGGFASGTVVNSGGVINASGEE504 pKa = 4.26TIYY507 pKa = 10.81YY508 pKa = 9.23PPGGPYY514 pKa = 10.46GIVFRR519 pKa = 11.84SEE521 pKa = 3.07VDD523 pKa = 3.9FAVVGSGGQLHH534 pKa = 6.89LFGAGIAKK542 pKa = 9.87KK543 pKa = 10.41AIINGGVMTVSGSVADD559 pKa = 3.96PTNDD563 pKa = 3.22PSAPAVDD570 pKa = 4.57ASAASGTVLNSGSLSVSDD588 pKa = 3.95GGIVTSTILNGGTLDD603 pKa = 4.4VLDD606 pKa = 4.28QGTANASTVLRR617 pKa = 11.84GATEE621 pKa = 3.88LVEE624 pKa = 4.55AGGIAGATTIAGGMLDD640 pKa = 4.62LKK642 pKa = 11.01AGAISDD648 pKa = 3.95DD649 pKa = 4.6AITFSGQGTLKK660 pKa = 10.2IEE662 pKa = 4.14QKK664 pKa = 10.54LVSGSSAFASQIKK677 pKa = 10.27GIALGDD683 pKa = 3.84QIDD686 pKa = 4.18LSGLSYY692 pKa = 10.88TSGATVTVSGSKK704 pKa = 9.15LTVSNGAASEE714 pKa = 4.34TFTLADD720 pKa = 3.33TSVTRR725 pKa = 11.84FGIAKK730 pKa = 9.66DD731 pKa = 3.51SSGGILLTAAALPAIAGAVSGQATSNQASINPFATVTITDD771 pKa = 4.0PNAAATDD778 pKa = 3.78SLIITLAGAAGILSGSGLISLGNGTYY804 pKa = 10.56EE805 pKa = 4.61LAATSAANLTAEE817 pKa = 4.48LHH819 pKa = 6.31ALTFVASPHH828 pKa = 6.4GDD830 pKa = 3.0GSVTTTFTLSDD841 pKa = 3.6TSSVGLTVSDD851 pKa = 3.95TNTSVTDD858 pKa = 2.99THH860 pKa = 6.44QAGTTTIDD868 pKa = 3.73GPASGYY874 pKa = 11.09AIIQGTTGNDD884 pKa = 3.04IIHH887 pKa = 7.26AYY889 pKa = 10.79GMFNTIHH896 pKa = 6.69GNGGSDD902 pKa = 3.56TIYY905 pKa = 10.54AGLYY909 pKa = 7.63GTVDD913 pKa = 3.62VPSGDD918 pKa = 3.52SAVYY922 pKa = 9.65LQGIVNHH929 pKa = 5.25VTGGYY934 pKa = 10.89GNVTVTGSTGATTIALGNGNDD955 pKa = 4.03TIDD958 pKa = 3.2ASGYY962 pKa = 10.58GNVITLGNGNDD973 pKa = 3.4IVHH976 pKa = 7.1PGDD979 pKa = 3.89GASQTTAGNGNDD991 pKa = 3.63LVTLSGYY998 pKa = 11.1GNTVMLGNGNDD1009 pKa = 3.42VVAGRR1014 pKa = 11.84DD1015 pKa = 3.45GANSVTLGDD1024 pKa = 4.03GNNTVNLGGMGNQITVGSGTNAIIAGSGSDD1054 pKa = 3.46NVVAGAGHH1062 pKa = 6.01DD1063 pKa = 3.82TIMLGGAANHH1073 pKa = 5.65VVLNGSQANVTNQIGQDD1090 pKa = 3.38VVTVNGGSDD1099 pKa = 3.06QFNFVGFGNQAIINGPAHH1117 pKa = 6.79VDD1119 pKa = 3.67IIDD1122 pKa = 3.94HH1123 pKa = 5.7GTGLTVSVNSSSQVDD1138 pKa = 4.18TISGFGNDD1146 pKa = 3.33TLGIVALANGVGDD1159 pKa = 3.86YY1160 pKa = 11.06HH1161 pKa = 8.24SVADD1165 pKa = 3.73IMSALRR1171 pKa = 11.84SDD1173 pKa = 3.74GHH1175 pKa = 6.51GGTLLALGSGSNAGSIDD1192 pKa = 3.72FVDD1195 pKa = 3.73TAISQLHH1202 pKa = 6.58ASNFMIVV1209 pKa = 2.81

Molecular weight: 118.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q89BY5|Q89BY5_BRADU N-(5'-phosphoribosyl)anthranilate isomerase OS=Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) OX=224911 GN=trpF PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84ARR21 pKa = 11.84LATAGGRR28 pKa = 11.84KK29 pKa = 8.95VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8253

0

8253

2616478

26

5685

317.0

34.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.422 ± 0.033

0.919 ± 0.01

5.466 ± 0.025

5.257 ± 0.031

3.737 ± 0.018

8.359 ± 0.031

2.057 ± 0.012

5.266 ± 0.022

3.609 ± 0.027

9.86 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.467 ± 0.014

2.76 ± 0.022

5.263 ± 0.026

3.187 ± 0.017

7.186 ± 0.039

5.819 ± 0.024

5.425 ± 0.038

7.416 ± 0.023

1.316 ± 0.012

2.209 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski