Synechococcus phage S-SSM5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 225 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3SKP2|E3SKP2_9CAUD Uncharacterized protein OS=Synechococcus phage S-SSM5 OX=445685 GN=SSSM5_203 PE=4 SV=1
MM1 pKa = 6.51TTAQRR6 pKa = 11.84FSSCLDD12 pKa = 3.12ILTDD16 pKa = 3.53AVDD19 pKa = 3.56RR20 pKa = 11.84QIILDD25 pKa = 3.69TEE27 pKa = 4.28YY28 pKa = 10.4PILYY32 pKa = 8.61NQVVKK37 pKa = 10.43FFEE40 pKa = 4.26EE41 pKa = 4.17KK42 pKa = 10.84GVDD45 pKa = 3.91FYY47 pKa = 12.06GDD49 pKa = 3.03VDD51 pKa = 3.75EE52 pKa = 6.95DD53 pKa = 4.59YY54 pKa = 11.28DD55 pKa = 3.74ILLNKK60 pKa = 10.12LEE62 pKa = 4.13QDD64 pKa = 3.58LFYY67 pKa = 11.19YY68 pKa = 10.65DD69 pKa = 3.35SS70 pKa = 3.78

Molecular weight: 8.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3SKC6|E3SKC6_9CAUD Uncharacterized protein OS=Synechococcus phage S-SSM5 OX=445685 GN=SSSM5_086 PE=4 SV=1
MM1 pKa = 7.55LRR3 pKa = 11.84MAVFLCPLNVKK14 pKa = 9.68IGHH17 pKa = 5.8YY18 pKa = 10.02PNLQRR23 pKa = 11.84FPDD26 pKa = 3.84ALNIIRR32 pKa = 11.84VILNGVYY39 pKa = 10.4KK40 pKa = 10.65KK41 pKa = 10.58ISEE44 pKa = 4.52TKK46 pKa = 10.46NGPQDD51 pKa = 3.29PRR53 pKa = 11.84RR54 pKa = 11.84KK55 pKa = 7.8TRR57 pKa = 11.84CSCMGNRR64 pKa = 11.84ATDD67 pKa = 3.1

Molecular weight: 7.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

225

0

225

57251

30

6171

254.4

28.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.125 ± 0.215

0.924 ± 0.105

6.491 ± 0.156

5.902 ± 0.312

4.314 ± 0.114

7.933 ± 0.357

1.635 ± 0.139

6.026 ± 0.176

5.942 ± 0.368

7.163 ± 0.179

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.14 ± 0.218

5.748 ± 0.18

3.893 ± 0.133

3.67 ± 0.1

4.047 ± 0.18

7.015 ± 0.209

7.789 ± 0.466

6.863 ± 0.201

1.155 ± 0.089

4.225 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski