Streptomyces nodosus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5863 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5DUQ8|A0A0B5DUQ8_9ACTN XRE family transcriptional regulator OS=Streptomyces nodosus OX=40318 GN=CP978_31190 PE=4 SV=1
MM1 pKa = 7.76KK2 pKa = 9.46ITVEE6 pKa = 3.83EE7 pKa = 4.72DD8 pKa = 3.26KK9 pKa = 11.37CCGAGQCVLIAPEE22 pKa = 4.32VFDD25 pKa = 5.17QRR27 pKa = 11.84DD28 pKa = 3.32DD29 pKa = 3.93DD30 pKa = 5.4GIVILLDD37 pKa = 3.3ATPAEE42 pKa = 4.25DD43 pKa = 3.6QYY45 pKa = 11.8EE46 pKa = 4.37QVLEE50 pKa = 4.14AAAVCPAACIHH61 pKa = 6.87LDD63 pKa = 3.72DD64 pKa = 5.01KK65 pKa = 11.85AA66 pKa = 4.27

Molecular weight: 7.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5DMD1|A0A0B5DMD1_9ACTN Uncharacterized protein OS=Streptomyces nodosus OX=40318 GN=SNOD_14575 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.75GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5863

0

5863

1903485

29

10917

324.7

34.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.159 ± 0.053

0.782 ± 0.008

5.947 ± 0.025

5.806 ± 0.033

2.76 ± 0.02

9.448 ± 0.033

2.344 ± 0.015

3.241 ± 0.024

2.16 ± 0.033

10.484 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.769 ± 0.015

1.751 ± 0.02

5.983 ± 0.03

2.776 ± 0.019

8.146 ± 0.039

5.143 ± 0.025

6.149 ± 0.027

8.563 ± 0.029

1.497 ± 0.012

2.085 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski