Cohnella kolymensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Cohnella

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3360 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C2UWW8|A0A0C2UWW8_9BACL Uncharacterized protein OS=Cohnella kolymensis OX=1590652 GN=SD71_04535 PE=4 SV=1
MM1 pKa = 7.8AKK3 pKa = 9.14FTYY6 pKa = 9.93VDD8 pKa = 3.79KK9 pKa = 10.01DD10 pKa = 3.4TCIACGACGATAPDD24 pKa = 3.47IYY26 pKa = 11.02DD27 pKa = 3.64YY28 pKa = 11.51DD29 pKa = 4.43DD30 pKa = 3.97EE31 pKa = 5.79GLAEE35 pKa = 4.8VIFKK39 pKa = 10.9ADD41 pKa = 3.48GNHH44 pKa = 6.41GNTEE48 pKa = 4.03IPEE51 pKa = 4.24DD52 pKa = 4.74LYY54 pKa = 11.66DD55 pKa = 4.58DD56 pKa = 4.95LQDD59 pKa = 4.96ASDD62 pKa = 4.13GCPTDD67 pKa = 3.73SIKK70 pKa = 11.13VADD73 pKa = 4.44SPFNMM78 pKa = 4.87

Molecular weight: 8.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C2R424|A0A0C2R424_9BACL Aminoacylase OS=Cohnella kolymensis OX=1590652 GN=SD71_08460 PE=4 SV=1
MM1 pKa = 7.61GPTFKK6 pKa = 10.87PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.57KK14 pKa = 8.17VHH16 pKa = 5.5GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSKK25 pKa = 10.5NGRR28 pKa = 11.84KK29 pKa = 8.92VLQARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.18GRR39 pKa = 11.84KK40 pKa = 8.88VLTAA44 pKa = 4.27

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3360

0

3360

940578

26

2774

279.9

31.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.694 ± 0.051

0.787 ± 0.016

5.232 ± 0.036

6.615 ± 0.048

4.052 ± 0.034

7.538 ± 0.036

2.115 ± 0.022

6.625 ± 0.041

5.23 ± 0.037

9.994 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.918 ± 0.024

3.655 ± 0.033

4.157 ± 0.029

3.714 ± 0.024

5.485 ± 0.041

6.157 ± 0.035

5.288 ± 0.038

7.345 ± 0.031

1.236 ± 0.018

3.164 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski