Clostridium collagenovorans DSM 3089

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium collagenovorans

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2997 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M5U0E8|A0A1M5U0E8_9CLOT Shikimate dehydrogenase (NADP(+)) OS=Clostridium collagenovorans DSM 3089 OX=1121306 GN=aroE PE=3 SV=1
MM1 pKa = 7.51INKK4 pKa = 9.56KK5 pKa = 10.35EE6 pKa = 4.03IIEE9 pKa = 4.13YY10 pKa = 10.68LSDD13 pKa = 3.71FQLEE17 pKa = 4.53CIKK20 pKa = 10.24EE21 pKa = 4.05IKK23 pKa = 9.84VEE25 pKa = 3.77KK26 pKa = 10.5DD27 pKa = 3.11LIIFKK32 pKa = 10.84FEE34 pKa = 4.41LEE36 pKa = 4.5LEE38 pKa = 4.31DD39 pKa = 5.07SEE41 pKa = 5.47IEE43 pKa = 4.03AAEE46 pKa = 3.96AFADD50 pKa = 4.4EE51 pKa = 4.61EE52 pKa = 4.57CEE54 pKa = 4.0EE55 pKa = 4.23PKK57 pKa = 10.62KK58 pKa = 10.81SEE60 pKa = 3.52EE61 pKa = 3.97WYY63 pKa = 10.06EE64 pKa = 4.04EE65 pKa = 3.85HH66 pKa = 6.81FLPYY70 pKa = 10.23LSDD73 pKa = 3.43MAIDD77 pKa = 4.37DD78 pKa = 4.37VEE80 pKa = 4.72EE81 pKa = 4.46MIQEE85 pKa = 4.24CAEE88 pKa = 4.11EE89 pKa = 4.22FDD91 pKa = 5.26LDD93 pKa = 3.99YY94 pKa = 11.25EE95 pKa = 5.41LIMLDD100 pKa = 3.31ISEE103 pKa = 4.64EE104 pKa = 4.29KK105 pKa = 10.23YY106 pKa = 11.15DD107 pKa = 4.0SIEE110 pKa = 3.73FLGAFLCNGKK120 pKa = 9.09EE121 pKa = 4.25VYY123 pKa = 10.4LEE125 pKa = 4.22EE126 pKa = 4.69YY127 pKa = 9.74ISEE130 pKa = 4.5III132 pKa = 4.26

Molecular weight: 15.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M5YMP2|A0A1M5YMP2_9CLOT Y_Y_Y domain-containing protein OS=Clostridium collagenovorans DSM 3089 OX=1121306 GN=SAMN02745196_03063 PE=4 SV=1
MM1 pKa = 7.77KK2 pKa = 8.13MTLQPKK8 pKa = 9.15KK9 pKa = 8.69RR10 pKa = 11.84QRR12 pKa = 11.84NKK14 pKa = 8.71EE15 pKa = 3.51HH16 pKa = 6.78GFRR19 pKa = 11.84KK20 pKa = 9.98RR21 pKa = 11.84MSTASGRR28 pKa = 11.84NVLKK32 pKa = 10.3RR33 pKa = 11.84RR34 pKa = 11.84KK35 pKa = 9.06RR36 pKa = 11.84KK37 pKa = 8.68GRR39 pKa = 11.84KK40 pKa = 9.1RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2997

0

2997

939149

39

3255

313.4

35.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.449 ± 0.043

1.261 ± 0.02

5.421 ± 0.036

7.862 ± 0.056

4.392 ± 0.035

6.316 ± 0.041

1.292 ± 0.017

9.788 ± 0.061

9.024 ± 0.046

9.33 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.65 ± 0.023

6.242 ± 0.048

2.701 ± 0.025

2.203 ± 0.019

3.272 ± 0.029

6.572 ± 0.037

4.861 ± 0.044

6.652 ± 0.038

0.621 ± 0.012

4.092 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski