Okra yellow crinkle virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A4D8I5|A4D8I5_9GEMI C4 protein OS=Okra yellow crinkle virus OX=401040 GN=C4 PE=3 SV=1
MM1 pKa = 7.35PRR3 pKa = 11.84AGRR6 pKa = 11.84FSIKK10 pKa = 10.18AKK12 pKa = 10.62NYY14 pKa = 8.83FLTFPKK20 pKa = 10.41CSLSKK25 pKa = 10.79EE26 pKa = 4.19DD27 pKa = 5.73ALGQLKK33 pKa = 10.42DD34 pKa = 3.98LQTPTNKK41 pKa = 10.09KK42 pKa = 8.99YY43 pKa = 10.85IKK45 pKa = 9.66ICRR48 pKa = 11.84EE49 pKa = 3.53LHH51 pKa = 6.26EE52 pKa = 4.93NGEE55 pKa = 4.28PHH57 pKa = 6.66LHH59 pKa = 6.03VLIQFEE65 pKa = 4.49GKK67 pKa = 10.12YY68 pKa = 10.4NCTNQRR74 pKa = 11.84FFDD77 pKa = 3.98LVSPSRR83 pKa = 11.84STHH86 pKa = 4.86FHH88 pKa = 6.77PNIQGAKK95 pKa = 9.25SSSDD99 pKa = 3.11VKK101 pKa = 11.24SYY103 pKa = 10.79IDD105 pKa = 3.7KK106 pKa = 11.33DD107 pKa = 3.49GDD109 pKa = 3.67TLEE112 pKa = 4.1WGEE115 pKa = 3.95FQVDD119 pKa = 3.21GRR121 pKa = 11.84SARR124 pKa = 11.84GGCQTANDD132 pKa = 4.06AAAEE136 pKa = 3.92ALNAGSADD144 pKa = 3.08AAMAIIRR151 pKa = 11.84EE152 pKa = 4.42KK153 pKa = 10.91LPKK156 pKa = 10.19DD157 pKa = 3.96YY158 pKa = 10.79IFQYY162 pKa = 11.19HH163 pKa = 5.53NLKK166 pKa = 10.92SNLDD170 pKa = 4.28RR171 pKa = 11.84IFQAPPEE178 pKa = 4.52VYY180 pKa = 10.24VSPFSSSSFNNVPEE194 pKa = 4.27EE195 pKa = 4.1LEE197 pKa = 3.69EE198 pKa = 3.88WAAEE202 pKa = 4.17NVVGAAARR210 pKa = 11.84PWRR213 pKa = 11.84PQSIVVEE220 pKa = 4.34GDD222 pKa = 2.92SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLGPHH237 pKa = 6.7NYY239 pKa = 10.01LCGHH243 pKa = 7.13IDD245 pKa = 3.96LSPRR249 pKa = 11.84VYY251 pKa = 11.1SNEE254 pKa = 2.95AWYY257 pKa = 10.82NVIDD261 pKa = 5.29DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.46FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.26YY287 pKa = 10.63GKK289 pKa = 9.49PIQIKK294 pKa = 10.42GGIPTIFLCNPGPTASYY311 pKa = 10.86KK312 pKa = 10.63EE313 pKa = 4.01YY314 pKa = 11.17LEE316 pKa = 4.14EE317 pKa = 5.24DD318 pKa = 3.12KK319 pKa = 11.46NAALKK324 pKa = 10.41AWAIKK329 pKa = 10.11NATFITLSEE338 pKa = 4.15PLYY341 pKa = 10.27STTDD345 pKa = 3.3QSPAPHH351 pKa = 5.85SQEE354 pKa = 4.25EE355 pKa = 4.53GSEE358 pKa = 3.99AA359 pKa = 3.7

Molecular weight: 40.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q06F30|Q06F30_9GEMI Replication enhancer OS=Okra yellow crinkle virus OX=401040 GN=C3 PE=3 SV=1
MM1 pKa = 8.27DD2 pKa = 4.36SRR4 pKa = 11.84TGEE7 pKa = 4.77LITARR12 pKa = 11.84QTTNGVFIWEE22 pKa = 4.59TPNPLYY28 pKa = 10.71FKK30 pKa = 9.66ITRR33 pKa = 11.84HH34 pKa = 6.32LIHH37 pKa = 6.92PFNRR41 pKa = 11.84PFHH44 pKa = 7.12IIDD47 pKa = 3.19VQIRR51 pKa = 11.84FNHH54 pKa = 5.9NLRR57 pKa = 11.84KK58 pKa = 9.83ALGIHH63 pKa = 5.96KK64 pKa = 9.99CFLNFRR70 pKa = 11.84VWTTLRR76 pKa = 11.84PQTGRR81 pKa = 11.84FLRR84 pKa = 11.84VFRR87 pKa = 11.84TQVLKK92 pKa = 10.91YY93 pKa = 10.13LNNLGVISINLVIRR107 pKa = 11.84AVAHH111 pKa = 4.93VLYY114 pKa = 10.71SVLLNTIDD122 pKa = 3.42VTEE125 pKa = 3.99SHH127 pKa = 6.94EE128 pKa = 4.21IKK130 pKa = 10.53FDD132 pKa = 3.31IYY134 pKa = 11.57

Molecular weight: 15.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1089

87

359

181.5

20.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.061 ± 1.131

2.112 ± 0.361

4.775 ± 0.567

4.683 ± 0.937

4.224 ± 0.525

5.142 ± 0.598

3.949 ± 0.533

5.234 ± 0.794

5.51 ± 0.813

7.071 ± 0.941

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.02 ± 0.513

5.693 ± 0.39

5.969 ± 0.552

4.316 ± 0.592

7.254 ± 1.26

8.448 ± 1.247

6.428 ± 1.109

5.693 ± 0.927

1.561 ± 0.19

3.857 ± 0.517
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski