Monosporascus sp. 5C6A

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Xylariomycetidae; Xylariales; Xylariales incertae sedis; Monosporascus; unclassified Monosporascus

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12533 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q4YVH5|A0A4Q4YVH5_9PEZI NACHT domain-containing protein OS=Monosporascus sp. 5C6A OX=2211642 GN=DL771_000030 PE=4 SV=1
MM1 pKa = 7.69EE2 pKa = 5.59GGGPAIEE9 pKa = 4.89GVDD12 pKa = 3.8SQRR15 pKa = 11.84DD16 pKa = 3.79PTPLVHH22 pKa = 7.38NDD24 pKa = 2.12IHH26 pKa = 5.79TQNVMIGPCVANPEE40 pKa = 4.23DD41 pKa = 4.04TEE43 pKa = 5.08HH44 pKa = 6.94YY45 pKa = 7.48ITPILKK51 pKa = 10.58LIDD54 pKa = 4.21FGSGGQDD61 pKa = 3.21LEE63 pKa = 5.8GDD65 pKa = 3.87DD66 pKa = 5.41GYY68 pKa = 11.69EE69 pKa = 4.05DD70 pKa = 3.63MQSNAIIDD78 pKa = 3.61NLQNIGYY85 pKa = 9.08IINALAVLNYY95 pKa = 10.22EE96 pKa = 4.66LDD98 pKa = 3.36ATTADD103 pKa = 4.01LVTEE107 pKa = 4.84PGHH110 pKa = 5.99PGIRR114 pKa = 11.84THH116 pKa = 6.98AAEE119 pKa = 5.23LLPDD123 pKa = 3.77SNGNDD128 pKa = 3.63PCPDD132 pKa = 3.61LDD134 pKa = 3.61EE135 pKa = 4.94SLRR138 pKa = 11.84TMICLCRR145 pKa = 11.84AVQPSNVPQIGALANMIMEE164 pKa = 5.71HH165 pKa = 5.29IQEE168 pKa = 4.79RR169 pKa = 11.84DD170 pKa = 3.48EE171 pKa = 3.89EE172 pKa = 4.91WYY174 pKa = 10.71GDD176 pKa = 3.69EE177 pKa = 4.59YY178 pKa = 11.32EE179 pKa = 4.61SDD181 pKa = 3.61EE182 pKa = 4.41QIAALFQTLLFDD194 pKa = 5.14ANASS198 pKa = 3.58

Molecular weight: 21.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q4Y458|A0A4Q4Y458_9PEZI Glycogen [starch] synthase OS=Monosporascus sp. 5C6A OX=2211642 GN=DL771_006853 PE=3 SV=1
MM1 pKa = 7.93RR2 pKa = 11.84SFSLAASAVRR12 pKa = 11.84TALSGPQSRR21 pKa = 11.84STIITTASKK30 pKa = 9.62RR31 pKa = 11.84TFSSLPQLRR40 pKa = 11.84PSILPLAHH48 pKa = 5.38GTVFRR53 pKa = 11.84PANKK57 pKa = 9.65ALSRR61 pKa = 11.84VSLTPSAPAGATGPVAMDD79 pKa = 4.43LVPKK83 pKa = 9.29TAITAHH89 pKa = 6.9PALASVQIRR98 pKa = 11.84CGPRR102 pKa = 11.84PTMARR107 pKa = 11.84SSRR110 pKa = 11.84LIRR113 pKa = 11.84KK114 pKa = 8.73RR115 pKa = 11.84RR116 pKa = 11.84HH117 pKa = 5.22GFLSRR122 pKa = 11.84LRR124 pKa = 11.84TRR126 pKa = 11.84NGRR129 pKa = 11.84RR130 pKa = 11.84TLQRR134 pKa = 11.84RR135 pKa = 11.84KK136 pKa = 9.97DD137 pKa = 3.42KK138 pKa = 10.75KK139 pKa = 10.63RR140 pKa = 11.84SVLSMM145 pKa = 4.25

Molecular weight: 15.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12533

0

12533

6089802

66

6400

485.9

53.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.5 ± 0.024

1.158 ± 0.008

5.875 ± 0.015

6.26 ± 0.023

3.516 ± 0.013

7.377 ± 0.022

2.345 ± 0.009

4.482 ± 0.015

4.49 ± 0.018

8.864 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.112 ± 0.008

3.454 ± 0.012

6.148 ± 0.024

3.873 ± 0.013

6.665 ± 0.021

7.69 ± 0.021

5.742 ± 0.017

6.306 ± 0.017

1.465 ± 0.009

2.677 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski