Escherichia phage Henu8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Tempevirinae; Hanrivervirus; Escherichia virus Henu8

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B8RPP0|A0A5B8RPP0_9CAUD Minor tail protein OS=Escherichia phage Henu8 OX=2596677 PE=4 SV=1
MM1 pKa = 7.74SDD3 pKa = 3.22YY4 pKa = 11.28SVIVGAEE11 pKa = 4.1SPGCMVDD18 pKa = 3.27SSIMVINGSFVCGDD32 pKa = 3.41EE33 pKa = 5.08FIAPGKK39 pKa = 9.8IVRR42 pKa = 11.84LSHH45 pKa = 5.55ITNGYY50 pKa = 9.54KK51 pKa = 9.72IASVNGDD58 pKa = 3.68LVIGVAVRR66 pKa = 11.84PPDD69 pKa = 3.35SCIYY73 pKa = 10.81EE74 pKa = 4.15EE75 pKa = 4.69GDD77 pKa = 3.41PVNVVSRR84 pKa = 11.84GSVWCLTSEE93 pKa = 4.54SEE95 pKa = 4.24APEE98 pKa = 4.21YY99 pKa = 10.36GDD101 pKa = 4.61KK102 pKa = 11.37VFVTQDD108 pKa = 2.18GDD110 pKa = 3.43AAYY113 pKa = 10.82SEE115 pKa = 4.84GDD117 pKa = 4.34LLNGWIFTGEE127 pKa = 4.08HH128 pKa = 5.02VKK130 pKa = 10.26VDD132 pKa = 3.63RR133 pKa = 11.84DD134 pKa = 3.82SYY136 pKa = 10.97IVGVSISAA144 pKa = 3.44

Molecular weight: 15.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B8RPX6|A0A5B8RPX6_9CAUD Putative portal protein OS=Escherichia phage Henu8 OX=2596677 PE=4 SV=1
MM1 pKa = 6.49QTPINEE7 pKa = 4.51RR8 pKa = 11.84NWNMLEE14 pKa = 4.1LNDD17 pKa = 4.02IRR19 pKa = 11.84VGTKK23 pKa = 9.98FRR25 pKa = 11.84VTWADD30 pKa = 3.72EE31 pKa = 4.22YY32 pKa = 11.11CGVSRR37 pKa = 11.84GQVVTVDD44 pKa = 3.73SIYY47 pKa = 10.65RR48 pKa = 11.84GCSKK52 pKa = 10.62DD53 pKa = 3.29FRR55 pKa = 11.84RR56 pKa = 11.84PRR58 pKa = 11.84IKK60 pKa = 10.15NGYY63 pKa = 8.77IITRR67 pKa = 11.84RR68 pKa = 11.84FGFDD72 pKa = 3.25HH73 pKa = 6.47YY74 pKa = 11.17CVVATQGVLIEE85 pKa = 4.1LQRR88 pKa = 11.84ISDD91 pKa = 3.56HH92 pKa = 6.57RR93 pKa = 11.84GCHH96 pKa = 4.96VKK98 pKa = 7.65TTKK101 pKa = 10.18IPSQRR106 pKa = 11.84SRR108 pKa = 11.84YY109 pKa = 8.4DD110 pKa = 2.98ARR112 pKa = 11.84RR113 pKa = 11.84MRR115 pKa = 11.84RR116 pKa = 11.84LARR119 pKa = 11.84NAIKK123 pKa = 10.27FKK125 pKa = 10.34KK126 pKa = 9.86HH127 pKa = 5.07GGNFYY132 pKa = 11.16RR133 pKa = 11.84MYY135 pKa = 10.86KK136 pKa = 10.38GIARR140 pKa = 11.84NAGKK144 pKa = 10.28

Molecular weight: 16.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

13679

61

1192

210.4

23.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.961 ± 0.448

1.418 ± 0.173

6.075 ± 0.243

6.843 ± 0.259

3.838 ± 0.17

7.318 ± 0.257

1.791 ± 0.25

6.506 ± 0.213

7.522 ± 0.32

6.419 ± 0.223

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.114 ± 0.212

4.876 ± 0.284

3.348 ± 0.237

3.845 ± 0.289

5.132 ± 0.224

6.375 ± 0.301

5.476 ± 0.271

7.033 ± 0.235

1.447 ± 0.108

3.663 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski