Mycobacterium virus Crimd

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E0YQM9|E0YQM9_9CAUD Uncharacterized protein OS=Mycobacterium virus Crimd OX=861045 GN=67 PE=4 SV=1
MM1 pKa = 7.59SSHH4 pKa = 6.63RR5 pKa = 11.84CAGDD9 pKa = 3.22DD10 pKa = 4.88CGFCAQRR17 pKa = 11.84IEE19 pKa = 4.05QAEE22 pKa = 4.04YY23 pKa = 11.16DD24 pKa = 4.19RR25 pKa = 11.84DD26 pKa = 4.25CPADD30 pKa = 4.77DD31 pKa = 4.06YY32 pKa = 11.62PDD34 pKa = 4.44YY35 pKa = 11.65YY36 pKa = 11.28DD37 pKa = 3.5GTT39 pKa = 4.14

Molecular weight: 4.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E0YQJ5|E0YQJ5_9CAUD Uncharacterized protein OS=Mycobacterium virus Crimd OX=861045 GN=33 PE=4 SV=1
MM1 pKa = 7.01TKK3 pKa = 10.04VVEE6 pKa = 4.91TILGMLVQVWAGVRR20 pKa = 11.84RR21 pKa = 11.84FAAEE25 pKa = 3.63RR26 pKa = 11.84LGIRR30 pKa = 11.84TWEE33 pKa = 4.03DD34 pKa = 2.89LRR36 pKa = 11.84LQIHH40 pKa = 5.7VLSPYY45 pKa = 10.75AVTAMVTWNIASEE58 pKa = 4.31DD59 pKa = 3.66KK60 pKa = 11.22AKK62 pKa = 11.0LIVGLVLAVASPALAFFNTRR82 pKa = 11.84DD83 pKa = 3.29GFRR86 pKa = 11.84RR87 pKa = 11.84WVYY90 pKa = 11.13GLLPPLQAFIVGFGWAQDD108 pKa = 3.7STLTPLMAAIVALLGGAMAAANTPSSRR135 pKa = 11.84GPKK138 pKa = 8.69DD139 pKa = 3.0TRR141 pKa = 11.84TAAVRR146 pKa = 3.49

Molecular weight: 15.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

18635

39

1278

196.2

21.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.271 ± 0.503

1.084 ± 0.144

6.67 ± 0.236

5.94 ± 0.273

2.613 ± 0.133

8.844 ± 0.446

2.163 ± 0.166

3.719 ± 0.18

3.461 ± 0.227

8.259 ± 0.244

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.093 ± 0.085

2.721 ± 0.175

5.694 ± 0.25

3.273 ± 0.136

7.175 ± 0.35

5.06 ± 0.208

5.763 ± 0.195

7.845 ± 0.278

2.082 ± 0.109

2.27 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski