Bacillus sp. SJS

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4050 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A176J4B3|A0A176J4B3_9BACI Glycos_transf_1 domain-containing protein OS=Bacillus sp. SJS OX=1423321 GN=AS29_015545 PE=4 SV=1
MM1 pKa = 7.12LTITSHH7 pKa = 5.06EE8 pKa = 4.24AEE10 pKa = 4.34LLEE13 pKa = 4.93DD14 pKa = 4.13PFGILPGDD22 pKa = 3.4RR23 pKa = 11.84YY24 pKa = 11.01EE25 pKa = 4.95FLLDD29 pKa = 3.93LDD31 pKa = 4.1VPEE34 pKa = 5.95DD35 pKa = 3.78DD36 pKa = 4.76EE37 pKa = 5.88LYY39 pKa = 10.69SEE41 pKa = 4.08NGIYY45 pKa = 10.54LRR47 pKa = 11.84VIFLLEE53 pKa = 3.88NEE55 pKa = 4.41QARR58 pKa = 11.84ITQYY62 pKa = 10.92QFYY65 pKa = 10.05EE66 pKa = 4.51KK67 pKa = 9.64EE68 pKa = 4.42TEE70 pKa = 3.8QSFDD74 pKa = 3.3FGLDD78 pKa = 3.35EE79 pKa = 4.54EE80 pKa = 4.71EE81 pKa = 4.2EE82 pKa = 4.45AMVASYY88 pKa = 10.64CLEE91 pKa = 4.06HH92 pKa = 8.26LPEE95 pKa = 4.42EE96 pKa = 4.59KK97 pKa = 10.57EE98 pKa = 4.07EE99 pKa = 4.09EE100 pKa = 4.12

Molecular weight: 11.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A176J2H4|A0A176J2H4_9BACI ABC transporter ATP-binding protein OS=Bacillus sp. SJS OX=1423321 GN=AS29_020195 PE=4 SV=1
MM1 pKa = 7.35RR2 pKa = 11.84TQSIRR7 pKa = 11.84TFLQNNRR14 pKa = 11.84IFQYY18 pKa = 10.25TLAILFLAVAFLAAVSIGTAAISPAEE44 pKa = 4.06IVSVLLGGNAASEE57 pKa = 4.34TNEE60 pKa = 4.28NILMSIRR67 pKa = 11.84IPRR70 pKa = 11.84VLLAGLVGAALALSGAAFQGLLRR93 pKa = 11.84NPLADD98 pKa = 4.57PYY100 pKa = 10.53TLGVSSGASLGAVSVIYY117 pKa = 10.31FGFQAFGSFSLPIVSIASGFLTMFGVLGFAYY148 pKa = 10.27LAQRR152 pKa = 11.84RR153 pKa = 11.84TSMEE157 pKa = 3.77TMILAGIIFNSFLGSFISLIIALSGEE183 pKa = 4.3EE184 pKa = 4.26LRR186 pKa = 11.84QVMNWLLGSVSMRR199 pKa = 11.84GWPYY203 pKa = 10.6VHH205 pKa = 6.99MILPFTAAGAFLLLMHH221 pKa = 6.52SRR223 pKa = 11.84EE224 pKa = 4.18LNAFAFGEE232 pKa = 4.03DD233 pKa = 3.36RR234 pKa = 11.84ARR236 pKa = 11.84HH237 pKa = 5.3IGVNVKK243 pKa = 10.06RR244 pKa = 11.84KK245 pKa = 8.95QFLIIISASMLTGSAVAVSGTIGFVGLVIPHH276 pKa = 5.62MTRR279 pKa = 11.84LLCGSDD285 pKa = 3.3HH286 pKa = 6.94RR287 pKa = 11.84HH288 pKa = 5.97LLPLSMIFGAGFLILTDD305 pKa = 4.04LLARR309 pKa = 11.84TVISPVEE316 pKa = 4.0LPIGIITAIAGAPVFAAILAAKK338 pKa = 9.57KK339 pKa = 10.27RR340 pKa = 11.84SSS342 pKa = 3.08

Molecular weight: 36.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4050

0

4050

1163448

27

1882

287.3

32.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.713 ± 0.043

0.693 ± 0.01

5.011 ± 0.03

7.613 ± 0.046

4.512 ± 0.032

7.137 ± 0.039

2.064 ± 0.019

7.326 ± 0.041

6.996 ± 0.031

9.803 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.903 ± 0.018

4.048 ± 0.025

3.745 ± 0.024

3.662 ± 0.026

4.198 ± 0.03

6.422 ± 0.033

5.181 ± 0.027

6.499 ± 0.032

1.035 ± 0.015

3.439 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski