Flavobacterium cerinum

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3673 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S3QFA5|A0A3S3QFA5_9FLAO Uncharacterized protein OS=Flavobacterium cerinum OX=2502784 GN=EPI11_16575 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.06KK3 pKa = 9.77IKK5 pKa = 10.38LILVSLFTVAMMLSCDD21 pKa = 4.72DD22 pKa = 4.93DD23 pKa = 6.67GGDD26 pKa = 3.44SKK28 pKa = 11.65INLEE32 pKa = 4.17VGAVPNIVKK41 pKa = 10.39SEE43 pKa = 3.95TDD45 pKa = 3.61DD46 pKa = 5.84LILNLVKK53 pKa = 10.49IQDD56 pKa = 4.07GEE58 pKa = 4.31NITIGFSIEE67 pKa = 3.8MAQGKK72 pKa = 9.54ISSLDD77 pKa = 3.32LVAFYY82 pKa = 10.76IKK84 pKa = 10.74GDD86 pKa = 3.42GSAEE90 pKa = 4.12KK91 pKa = 10.19IVLASNVTTFPTSMTLTKK109 pKa = 10.34QQLIDD114 pKa = 3.73KK115 pKa = 7.35FTVLTDD121 pKa = 3.48DD122 pKa = 3.93TDD124 pKa = 4.13FEE126 pKa = 5.77LGDD129 pKa = 4.03EE130 pKa = 4.91LIITADD136 pKa = 3.35LTLANGTLLKK146 pKa = 10.54LHH148 pKa = 6.9NDD150 pKa = 4.32DD151 pKa = 4.82GSQNYY156 pKa = 8.51GQDD159 pKa = 3.04IANSPIYY166 pKa = 10.56SVAQSYY172 pKa = 9.87LVACPSDD179 pKa = 3.41LAGTYY184 pKa = 10.46SVITSGEE191 pKa = 4.2STDD194 pKa = 4.58AGPTPDD200 pKa = 4.43EE201 pKa = 4.53NPITDD206 pKa = 3.77YY207 pKa = 11.07PFTVTLTDD215 pKa = 3.52KK216 pKa = 11.26GAGSYY221 pKa = 10.52EE222 pKa = 3.83MSDD225 pKa = 3.38AYY227 pKa = 10.63GGLYY231 pKa = 9.9LLWYY235 pKa = 9.76DD236 pKa = 4.19MYY238 pKa = 11.3GITPDD243 pKa = 3.94HH244 pKa = 7.09SIATIKK250 pKa = 10.38DD251 pKa = 3.34VCGVLSASYY260 pKa = 9.86KK261 pKa = 10.58GPFTEE266 pKa = 5.02SVTLSGMVNDD276 pKa = 5.32DD277 pKa = 3.64GTLTIHH283 pKa = 6.97WEE285 pKa = 3.88NEE287 pKa = 3.77YY288 pKa = 11.3GDD290 pKa = 4.8FGDD293 pKa = 5.56SVYY296 pKa = 11.02TKK298 pKa = 10.84NN299 pKa = 4.16

Molecular weight: 32.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S3U465|A0A3S3U465_9FLAO DUF4256 domain-containing protein OS=Flavobacterium cerinum OX=2502784 GN=EPI11_03155 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.91HH17 pKa = 4.39GFMDD21 pKa = 4.5RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.19GRR40 pKa = 11.84HH41 pKa = 5.41KK42 pKa = 10.14LTVSSEE48 pKa = 3.92PRR50 pKa = 11.84HH51 pKa = 5.77KK52 pKa = 10.61KK53 pKa = 9.84

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3673

0

3673

1213498

24

5527

330.4

37.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.002 ± 0.054

0.818 ± 0.018

5.397 ± 0.033

6.164 ± 0.056

5.026 ± 0.033

6.636 ± 0.047

1.662 ± 0.023

7.815 ± 0.043

7.556 ± 0.076

8.967 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.305 ± 0.025

6.071 ± 0.047

3.573 ± 0.033

3.317 ± 0.022

3.227 ± 0.031

6.332 ± 0.042

6.527 ± 0.1

6.444 ± 0.037

1.016 ± 0.015

4.145 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski