Klebsiella phage ST846-OXA48phi9.1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 38 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482IM14|A0A482IM14_9CAUD Uncharacterized protein OS=Klebsiella phage ST846-OXA48phi9.1 OX=2510488 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 9.8NLKK5 pKa = 9.17IAYY8 pKa = 9.39VDD10 pKa = 3.56EE11 pKa = 4.49KK12 pKa = 11.1LVAIEE17 pKa = 5.21CDD19 pKa = 3.58GLSCSSLPFSEE30 pKa = 5.39FPIDD34 pKa = 3.79STALTLPQLMLEE46 pKa = 4.46DD47 pKa = 4.88AYY49 pKa = 10.95AATSS53 pKa = 3.27

Molecular weight: 5.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482IJM0|A0A482IJM0_9CAUD Portal protein OS=Klebsiella phage ST846-OXA48phi9.1 OX=2510488 PE=4 SV=1
MM1 pKa = 7.28YY2 pKa = 10.75RR3 pKa = 11.84MDD5 pKa = 4.69KK6 pKa = 9.8LTTGIAYY13 pKa = 7.53GTSAGNAGFWMLQLLDD29 pKa = 4.46KK30 pKa = 11.03VSPSQWAAIGVLGSLVFGLLTYY52 pKa = 8.49LTNVYY57 pKa = 10.08FKK59 pKa = 10.82IKK61 pKa = 10.04DD62 pKa = 3.68DD63 pKa = 3.41RR64 pKa = 11.84RR65 pKa = 11.84KK66 pKa = 10.01AARR69 pKa = 11.84SGDD72 pKa = 3.58GKK74 pKa = 10.74QSEE77 pKa = 4.7TT78 pKa = 3.84

Molecular weight: 8.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

38

0

38

11819

44

4234

311.0

33.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.356 ± 0.792

0.888 ± 0.3

5.753 ± 0.228

5.212 ± 0.585

2.953 ± 0.312

7.759 ± 0.357

1.227 ± 0.362

5.576 ± 0.583

5.203 ± 0.522

8.571 ± 0.606

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.335 ± 0.427

4.823 ± 0.652

3.52 ± 0.437

5.077 ± 0.361

5.11 ± 0.323

7.429 ± 0.665

7.666 ± 1.292

6.405 ± 0.384

1.447 ± 0.179

2.691 ± 0.215
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski