Larimichthys crocea (Large yellow croaker) (Pseudosciaena crocea)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala;

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23960 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G0HV70|A0A6G0HV70_LARCR Adenylosuccinate lyase OS=Larimichthys crocea OX=215358 GN=D5F01_LYC18357 PE=3 SV=1
MM1 pKa = 7.58PAVNLIKK8 pKa = 10.31VTTNPAQTDD17 pKa = 3.14DD18 pKa = 3.64SQRR21 pKa = 11.84YY22 pKa = 6.68KK23 pKa = 10.77TDD25 pKa = 3.17VNPLTRR31 pKa = 11.84PSGLDD36 pKa = 3.17NLIEE40 pKa = 4.41EE41 pKa = 4.36DD42 pKa = 4.13TEE44 pKa = 4.17PKK46 pKa = 10.16EE47 pKa = 3.9EE48 pKa = 4.06VMGVFSSYY56 pKa = 11.01NGNPADD62 pKa = 3.35QYY64 pKa = 11.79ARR66 pKa = 11.84VSKK69 pKa = 10.67ISVDD73 pKa = 3.65SFDD76 pKa = 4.22SSSTSSGEE84 pKa = 4.2CYY86 pKa = 10.4EE87 pKa = 4.08NTSNYY92 pKa = 7.03VTPEE96 pKa = 4.13PGPSQSYY103 pKa = 8.09TVNDD107 pKa = 4.25PGDD110 pKa = 3.67FPSGYY115 pKa = 10.07RR116 pKa = 11.84NNQQTTDD123 pKa = 3.62QHH125 pKa = 5.72WLNPAEE131 pKa = 4.28VDD133 pKa = 3.54DD134 pKa = 5.69GIYY137 pKa = 10.72SPVSPDD143 pKa = 3.39QDD145 pKa = 3.4PSSDD149 pKa = 4.72DD150 pKa = 4.36DD151 pKa = 4.65YY152 pKa = 12.05DD153 pKa = 6.25DD154 pKa = 4.41IGAA157 pKa = 4.41

Molecular weight: 17.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G0J7C5|A0A6G0J7C5_LARCR RNA helicase OS=Larimichthys crocea OX=215358 GN=D5F01_LYC01755 PE=3 SV=1
MM1 pKa = 7.61LLLGVHH7 pKa = 6.47TKK9 pKa = 10.49RR10 pKa = 11.84KK11 pKa = 6.46TLRR14 pKa = 11.84LALTNRR20 pKa = 11.84ITNRR24 pKa = 11.84NINRR28 pKa = 11.84TINSNINKK36 pKa = 6.18TTNRR40 pKa = 11.84TLNKK44 pKa = 6.38TTNRR48 pKa = 11.84TINKK52 pKa = 5.6TTNRR56 pKa = 11.84TINKK60 pKa = 6.0TTNKK64 pKa = 10.01NINRR68 pKa = 11.84TITKK72 pKa = 5.86TTNRR76 pKa = 11.84TINKK80 pKa = 7.91TINKK84 pKa = 6.12TTNKK88 pKa = 9.26TINKK92 pKa = 5.65TTNRR96 pKa = 11.84TINKK100 pKa = 5.52TTNRR104 pKa = 11.84NIKK107 pKa = 5.99TTNRR111 pKa = 11.84TTNRR115 pKa = 11.84TTNRR119 pKa = 11.84TINKK123 pKa = 5.52TTNRR127 pKa = 11.84NIKK130 pKa = 6.05TTNRR134 pKa = 11.84NIKK137 pKa = 6.12TTNRR141 pKa = 11.84TINKK145 pKa = 5.52TTNRR149 pKa = 11.84NIKK152 pKa = 9.41NHH154 pKa = 4.75QQKK157 pKa = 9.24HH158 pKa = 4.32QQNHH162 pKa = 5.15HH163 pKa = 5.78QNHH166 pKa = 4.89QQNHH170 pKa = 4.3QQNHH174 pKa = 4.39QQNHH178 pKa = 4.23QQNHH182 pKa = 4.82QEE184 pKa = 3.87NDD186 pKa = 3.61QQKK189 pKa = 9.0HH190 pKa = 4.4QQNHH194 pKa = 5.15HH195 pKa = 5.78QNHH198 pKa = 4.89QQNHH202 pKa = 4.3QQNHH206 pKa = 4.12QQNYY210 pKa = 5.49QQNDD214 pKa = 3.65QQKK217 pKa = 8.47HH218 pKa = 4.19QQNHH222 pKa = 5.15HH223 pKa = 5.78QNHH226 pKa = 4.89QQNHH230 pKa = 4.3QQNHH234 pKa = 4.04QQNINKK240 pKa = 6.62TTNRR244 pKa = 11.84TINKK248 pKa = 5.6TTNRR252 pKa = 11.84TINKK256 pKa = 5.51TTNRR260 pKa = 11.84TTNRR264 pKa = 11.84TINKK268 pKa = 6.14TTNKK272 pKa = 9.26TINKK276 pKa = 5.65TTNRR280 pKa = 11.84TITKK284 pKa = 5.29TTNRR288 pKa = 11.84TTNRR292 pKa = 11.84TINKK296 pKa = 6.14TTNKK300 pKa = 9.26TINKK304 pKa = 5.55TTNRR308 pKa = 11.84NIKK311 pKa = 5.99TTNRR315 pKa = 11.84TTNRR319 pKa = 11.84TINKK323 pKa = 5.52TTNRR327 pKa = 11.84NIKK330 pKa = 6.12TTNRR334 pKa = 11.84TINKK338 pKa = 5.52TTNRR342 pKa = 11.84NIKK345 pKa = 5.99TTNRR349 pKa = 11.84TTNRR353 pKa = 11.84TINKK357 pKa = 5.52TTNRR361 pKa = 11.84NIKK364 pKa = 6.12TTNRR368 pKa = 11.84TINKK372 pKa = 5.52TTNRR376 pKa = 11.84NIKK379 pKa = 5.99TTNRR383 pKa = 11.84TTNRR387 pKa = 11.84TINKK391 pKa = 5.51TTNRR395 pKa = 11.84TTNRR399 pKa = 11.84TINKK403 pKa = 5.52TTNRR407 pKa = 11.84NIKK410 pKa = 6.07TTNRR414 pKa = 11.84NINRR418 pKa = 11.84TINRR422 pKa = 11.84TINRR426 pKa = 11.84TTNKK430 pKa = 5.61TTNRR434 pKa = 11.84KK435 pKa = 8.92

Molecular weight: 51.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23959

1

23960

13034180

150

16976

544.0

60.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.594 ± 0.015

2.198 ± 0.013

5.244 ± 0.012

6.933 ± 0.023

3.425 ± 0.012

6.327 ± 0.022

2.717 ± 0.009

4.228 ± 0.01

5.547 ± 0.017

9.38 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.404 ± 0.008

3.816 ± 0.013

5.847 ± 0.02

4.823 ± 0.015

5.795 ± 0.016

8.724 ± 0.021

5.84 ± 0.014

6.331 ± 0.015

1.158 ± 0.005

2.668 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski