Halorubrum phage CGphi46

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 4.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9TQ51|R9TQ51_9CAUD Uncharacterized protein OS=Halorubrum phage CGphi46 OX=754066 GN=HALG_00004 PE=4 SV=1
MM1 pKa = 7.4TGAGSATAAYY11 pKa = 9.6AVEE14 pKa = 4.09NAYY17 pKa = 10.53GEE19 pKa = 4.69LPADD23 pKa = 3.98PTWIQPGLNVTVSEE37 pKa = 4.45LSVDD41 pKa = 3.55QALSRR46 pKa = 11.84IRR48 pKa = 11.84TPDD51 pKa = 3.27DD52 pKa = 3.33PTPVGSRR59 pKa = 11.84LGNFEE64 pKa = 3.66GALAVSWDD72 pKa = 3.42LTGANFHH79 pKa = 7.1DD80 pKa = 5.32LVFADD85 pKa = 5.12GGTALPSAPTRR96 pKa = 11.84APSATWYY103 pKa = 10.81FDD105 pKa = 3.02VTLPDD110 pKa = 3.45GTTEE114 pKa = 3.75PRR116 pKa = 11.84TPTGTIVPDD125 pKa = 3.63ASITYY130 pKa = 8.23EE131 pKa = 3.66QGEE134 pKa = 4.34AVGVEE139 pKa = 3.94LTMLYY144 pKa = 10.66GDD146 pKa = 4.59EE147 pKa = 4.97PDD149 pKa = 5.59DD150 pKa = 3.65VTAPADD156 pKa = 3.85AEE158 pKa = 4.21IQTPSQDD165 pKa = 3.06DD166 pKa = 4.28VYY168 pKa = 10.59SFHH171 pKa = 6.82GASFSVDD178 pKa = 3.22TLNQPLMSSATLSLTGLARR197 pKa = 11.84FRR199 pKa = 11.84RR200 pKa = 11.84GQDD203 pKa = 2.85RR204 pKa = 11.84HH205 pKa = 6.53PYY207 pKa = 10.03DD208 pKa = 3.72AVADD212 pKa = 5.15AIAPEE217 pKa = 4.18FQTDD221 pKa = 3.25AVFTEE226 pKa = 4.28RR227 pKa = 11.84DD228 pKa = 3.5QLALAVDD235 pKa = 4.66DD236 pKa = 4.56VDD238 pKa = 4.21TPAPEE243 pKa = 4.41TVGSVPAEE251 pKa = 3.75FGLEE255 pKa = 3.83NGQGDD260 pKa = 4.8TITYY264 pKa = 8.43TLEE267 pKa = 4.15GCSPANYY274 pKa = 9.79AWSDD278 pKa = 3.59LVAPDD283 pKa = 4.56ADD285 pKa = 3.6LSEE288 pKa = 5.41PITYY292 pKa = 9.93QVDD295 pKa = 3.79YY296 pKa = 11.26VSAEE300 pKa = 4.02EE301 pKa = 4.14VTAA304 pKa = 4.62

Molecular weight: 32.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9TML6|R9TML6_9CAUD Uncharacterized protein OS=Halorubrum phage CGphi46 OX=754066 GN=HALG_00028 PE=4 SV=1
MM1 pKa = 7.02STVNTKK7 pKa = 9.91QVVVDD12 pKa = 3.87GDD14 pKa = 3.57ARR16 pKa = 11.84DD17 pKa = 3.55IPRR20 pKa = 11.84DD21 pKa = 3.5PNRR24 pKa = 11.84FRR26 pKa = 11.84PTTHH30 pKa = 6.52FGQRR34 pKa = 11.84LRR36 pKa = 11.84EE37 pKa = 4.01RR38 pKa = 11.84VPSADD43 pKa = 3.37RR44 pKa = 11.84NRR46 pKa = 11.84VVRR49 pKa = 11.84EE50 pKa = 3.64CFEE53 pKa = 4.61RR54 pKa = 11.84GVCRR58 pKa = 11.84GTTPARR64 pKa = 11.84SVDD67 pKa = 3.53RR68 pKa = 11.84PGVAFQTFEE77 pKa = 3.85FEE79 pKa = 4.53ARR81 pKa = 11.84FSADD85 pKa = 2.69SLMGSEE91 pKa = 4.37TARR94 pKa = 11.84TYY96 pKa = 11.41ALVVGVVRR104 pKa = 11.84EE105 pKa = 4.1AFRR108 pKa = 11.84NPRR111 pKa = 11.84KK112 pKa = 9.62KK113 pKa = 9.87HH114 pKa = 5.06RR115 pKa = 11.84ALTIVRR121 pKa = 11.84RR122 pKa = 11.84DD123 pKa = 3.49DD124 pKa = 3.57GGQRR128 pKa = 3.3

Molecular weight: 14.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

12159

43

1124

221.1

24.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.042 ± 0.361

0.592 ± 0.126

9.483 ± 0.372

8.94 ± 0.384

2.599 ± 0.13

8.619 ± 0.468

2.081 ± 0.19

3.775 ± 0.191

2.073 ± 0.2

7.073 ± 0.358

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.612 ± 0.159

2.78 ± 0.237

4.721 ± 0.186

3.306 ± 0.213

6.777 ± 0.294

6.16 ± 0.273

6.999 ± 0.364

7.764 ± 0.266

1.702 ± 0.133

2.903 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski