Haloactinopolyspora alba

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Jiangellales; Jiangellaceae; Haloactinopolyspora

Average proteome isoelectric point is 5.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5159 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P8DX69|A0A2P8DX69_9ACTN Carbohydrate ABC transporter membrane protein 2 (CUT1 family) OS=Haloactinopolyspora alba OX=648780 GN=CLV30_1126 PE=3 SV=1
MM1 pKa = 7.58RR2 pKa = 11.84PSRR5 pKa = 11.84SRR7 pKa = 11.84SSAAIGASVTALAMIAVAIPAQADD31 pKa = 3.46DD32 pKa = 4.8HH33 pKa = 6.78EE34 pKa = 4.8NSPGEE39 pKa = 4.0PTITLPINDD48 pKa = 3.78SAAGGTYY55 pKa = 10.54DD56 pKa = 4.24QDD58 pKa = 3.27FTRR61 pKa = 11.84AYY63 pKa = 10.41AGEE66 pKa = 4.42TPDD69 pKa = 3.43GTPVYY74 pKa = 10.29VYY76 pKa = 10.69VPAGTPLDD84 pKa = 3.91GSAPAGVPSLDD95 pKa = 3.39PQFDD99 pKa = 4.18HH100 pKa = 7.14NPDD103 pKa = 3.88DD104 pKa = 4.01EE105 pKa = 4.71FVPCEE110 pKa = 3.88TATAGQFTLTQAQIDD125 pKa = 4.21YY126 pKa = 10.16VGDD129 pKa = 3.58MLAEE133 pKa = 4.45RR134 pKa = 11.84IVAVDD139 pKa = 3.71EE140 pKa = 4.3EE141 pKa = 5.11HH142 pKa = 6.88FGAMDD147 pKa = 4.0AADD150 pKa = 4.4PSDD153 pKa = 3.96PASDD157 pKa = 3.63SLVMLVYY164 pKa = 10.46NVQDD168 pKa = 3.44DD169 pKa = 4.52AYY171 pKa = 9.46YY172 pKa = 10.85DD173 pKa = 3.91CAATSYY179 pKa = 9.15TAGYY183 pKa = 7.64FAPDD187 pKa = 5.03FIDD190 pKa = 3.43SSGMNTIVVDD200 pKa = 4.01GLDD203 pKa = 3.01WANRR207 pKa = 11.84VGPNDD212 pKa = 3.68AEE214 pKa = 4.23WRR216 pKa = 11.84DD217 pKa = 3.47GDD219 pKa = 4.03PANDD223 pKa = 3.59RR224 pKa = 11.84PEE226 pKa = 4.58LYY228 pKa = 10.04EE229 pKa = 3.88GVVAHH234 pKa = 6.13EE235 pKa = 4.75LEE237 pKa = 4.83HH238 pKa = 6.9LLHH241 pKa = 6.75NYY243 pKa = 10.07GDD245 pKa = 4.06GGEE248 pKa = 4.34LSWVDD253 pKa = 3.59EE254 pKa = 4.31GLADD258 pKa = 5.01LAVFLNGFDD267 pKa = 3.79AGGSHH272 pKa = 7.53LSNHH276 pKa = 4.28QVFYY280 pKa = 11.23EE281 pKa = 4.29EE282 pKa = 4.26TSLTRR287 pKa = 11.84WGGSLANYY295 pKa = 8.39GAAFTYY301 pKa = 9.16FQYY304 pKa = 10.98LWEE307 pKa = 4.13QAGGNGDD314 pKa = 3.77GTFTPDD320 pKa = 3.08QQYY323 pKa = 11.09DD324 pKa = 3.39AAAGDD329 pKa = 4.37LLIKK333 pKa = 9.85TIFEE337 pKa = 4.01EE338 pKa = 4.47QANGMAGVQNAIDD351 pKa = 3.88TFNAATGSDD360 pKa = 3.7LRR362 pKa = 11.84SAEE365 pKa = 4.35ALFKK369 pKa = 10.22DD370 pKa = 3.56WSVAVYY376 pKa = 10.67LDD378 pKa = 4.72DD379 pKa = 4.34EE380 pKa = 4.34QSPVYY385 pKa = 9.98DD386 pKa = 3.55IKK388 pKa = 11.4AFDD391 pKa = 4.14FGDD394 pKa = 4.21PEE396 pKa = 4.29DD397 pKa = 4.63TSWTMDD403 pKa = 3.22IADD406 pKa = 3.55DD407 pKa = 4.21TFWGGRR413 pKa = 11.84GSYY416 pKa = 9.7QGSQPEE422 pKa = 4.46AKK424 pKa = 9.48WSRR427 pKa = 11.84LKK429 pKa = 10.71NRR431 pKa = 11.84PDD433 pKa = 3.07TTAVPFGMSVEE444 pKa = 4.27RR445 pKa = 11.84FRR447 pKa = 11.84NPGPSVRR454 pKa = 11.84VDD456 pKa = 3.74FDD458 pKa = 4.33GDD460 pKa = 3.55DD461 pKa = 3.63RR462 pKa = 11.84TQVAPHH468 pKa = 6.8AGDD471 pKa = 3.47THH473 pKa = 6.8WYY475 pKa = 9.64GGYY478 pKa = 10.11EE479 pKa = 4.07SQADD483 pKa = 3.45NVLNVDD489 pKa = 4.02AGTSPSTLDD498 pKa = 3.16FWSWNFIEE506 pKa = 4.85QGWDD510 pKa = 3.21YY511 pKa = 11.43GFVEE515 pKa = 4.48ALVGGEE521 pKa = 4.07WVTVPLVDD529 pKa = 5.5DD530 pKa = 4.52EE531 pKa = 5.38GNTVTTDD538 pKa = 4.28DD539 pKa = 5.04DD540 pKa = 3.83PHH542 pKa = 7.9GNNTEE547 pKa = 4.32GNGLTGTSGGEE558 pKa = 4.02YY559 pKa = 10.35FVDD562 pKa = 3.79DD563 pKa = 4.16PVYY566 pKa = 11.09VHH568 pKa = 6.72LTAQLPAGATDD579 pKa = 2.98VRR581 pKa = 11.84FRR583 pKa = 11.84YY584 pKa = 8.58STDD587 pKa = 2.83AAYY590 pKa = 10.92LDD592 pKa = 4.21TGWFVDD598 pKa = 3.97DD599 pKa = 3.96VRR601 pKa = 11.84VDD603 pKa = 3.47GAEE606 pKa = 3.9ASVDD610 pKa = 3.19SDD612 pKa = 3.28AGHH615 pKa = 6.24WTRR618 pKa = 11.84TTGVQEE624 pKa = 4.32NNWTVQTIAACDD636 pKa = 3.85LTPGTTLPGEE646 pKa = 4.47SSDD649 pKa = 3.6GAGNHH654 pKa = 5.53VYY656 pKa = 10.39RR657 pKa = 11.84FEE659 pKa = 6.17GDD661 pKa = 3.1AFEE664 pKa = 4.67QGGLEE669 pKa = 4.23TKK671 pKa = 10.18CANGNQDD678 pKa = 4.15DD679 pKa = 4.25FAVTISNLPTGDD691 pKa = 3.92LTFLDD696 pKa = 3.43ADD698 pKa = 3.79YY699 pKa = 10.31TFRR702 pKa = 11.84VANTGNTKK710 pKa = 10.52

Molecular weight: 76.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P8DWF0|A0A2P8DWF0_9ACTN Argininosuccinate lyase OS=Haloactinopolyspora alba OX=648780 GN=CLV30_11334 PE=4 SV=1
MM1 pKa = 7.41VKK3 pKa = 9.03RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SIVTSRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.7GRR40 pKa = 11.84AKK42 pKa = 10.68LSAA45 pKa = 3.92

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5159

0

5159

1669478

25

3281

323.6

34.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.108 ± 0.049

0.659 ± 0.009

7.074 ± 0.033

5.898 ± 0.032

2.74 ± 0.019

9.31 ± 0.034

2.27 ± 0.016

3.217 ± 0.023

1.446 ± 0.02

9.622 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.852 ± 0.013

1.8 ± 0.02

5.741 ± 0.027

2.689 ± 0.017

8.007 ± 0.039

5.284 ± 0.021

6.25 ± 0.028

9.431 ± 0.038

1.558 ± 0.015

2.044 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski