Protochlamydia amoebophila (strain UWE25)

Taxonomy: cellular organisms; Bacteria; PVC group; Chlamydiae; Chlamydiia; Parachlamydiales; Parachlamydiaceae; Candidatus Protochlamydia; Candidatus Protochlamydia amoebophila

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1879 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6MCQ1|Q6MCQ1_PARUW Uncharacterized protein OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=PC_RS04455 PE=4 SV=1
MM1 pKa = 7.55KK2 pKa = 10.35VLNYY6 pKa = 9.6LLCLFICLTALVYY19 pKa = 10.78SEE21 pKa = 4.21IKK23 pKa = 10.12SVKK26 pKa = 10.46DD27 pKa = 4.79KK28 pKa = 10.96ICCDD32 pKa = 3.85LNASSIVEE40 pKa = 4.0VTQDD44 pKa = 3.26NFKK47 pKa = 11.01QLIFPEE53 pKa = 4.48KK54 pKa = 9.97PVILMIYY61 pKa = 10.23AEE63 pKa = 4.21WCGCCCRR70 pKa = 11.84FQPILEE76 pKa = 4.33TFSEE80 pKa = 4.47SFKK83 pKa = 10.63EE84 pKa = 4.21SIRR87 pKa = 11.84FAKK90 pKa = 10.4LNYY93 pKa = 10.26DD94 pKa = 3.98EE95 pKa = 4.85EE96 pKa = 4.63TDD98 pKa = 3.87LADD101 pKa = 4.81SFDD104 pKa = 3.56INYY107 pKa = 8.82FPTFIFIDD115 pKa = 3.54SGIVMDD121 pKa = 4.78KK122 pKa = 10.25TDD124 pKa = 4.39EE125 pKa = 4.29IDD127 pKa = 3.47TQEE130 pKa = 4.11EE131 pKa = 4.45LEE133 pKa = 4.29SQIFAWINQLNN144 pKa = 3.5

Molecular weight: 16.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6MAS4|Q6MAS4_PARUW DNA helicase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=PC_RS07675 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.23QPSKK9 pKa = 9.61RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84SSEE15 pKa = 3.58HH16 pKa = 6.23GFLKK20 pKa = 10.75RR21 pKa = 11.84MGTANGRR28 pKa = 11.84KK29 pKa = 8.74IISRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84HH37 pKa = 5.42GRR39 pKa = 11.84KK40 pKa = 8.91QLTRR44 pKa = 11.84VV45 pKa = 3.2

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1879

0

1879

644693

20

2402

343.1

38.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.488 ± 0.057

1.311 ± 0.019

4.587 ± 0.035

6.759 ± 0.055

5.031 ± 0.045

5.504 ± 0.054

2.528 ± 0.03

7.653 ± 0.05

7.117 ± 0.055

11.556 ± 0.087

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.039 ± 0.023

4.735 ± 0.04

3.94 ± 0.031

5.0 ± 0.04

4.135 ± 0.04

6.7 ± 0.036

5.123 ± 0.035

5.244 ± 0.045

1.139 ± 0.02

3.41 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski