Roseiarcus fermentans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Roseiarcaceae; Roseiarcus

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6190 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A366F0Z4|A0A366F0Z4_9RHIZ Putative NADPH-quinone reductase OS=Roseiarcus fermentans OX=1473586 GN=DFR50_12754 PE=4 SV=1
MM1 pKa = 7.47YY2 pKa = 10.26SATQINMLRR11 pKa = 11.84DD12 pKa = 3.78LDD14 pKa = 4.15SGAPLPGIDD23 pKa = 3.83TEE25 pKa = 4.46VGNLQSEE32 pKa = 4.86SSLEE36 pKa = 4.28TPSSIPTTSSNQPFYY51 pKa = 11.27YY52 pKa = 10.29DD53 pKa = 3.44PSKK56 pKa = 10.75NTVFVQQAGAVLNGYY71 pKa = 9.97DD72 pKa = 3.53FGSANVVVVASDD84 pKa = 3.46VTIEE88 pKa = 3.8NCTFDD93 pKa = 3.45GTGGSFAIGAYY104 pKa = 7.2NTAWSPATTDD114 pKa = 3.19TTVTHH119 pKa = 6.04CTFVGDD125 pKa = 4.11PSSSLLAVINSPTGSATITDD145 pKa = 3.69NAFLDD150 pKa = 4.06TAGDD154 pKa = 4.32GVWADD159 pKa = 3.48GGGLISGNYY168 pKa = 8.98FSQAGNDD175 pKa = 3.99GAAGHH180 pKa = 7.44PDD182 pKa = 4.76GIWLSNSTSPMTVSDD197 pKa = 4.88NFIDD201 pKa = 3.92WSQNPAATGSTNDD214 pKa = 4.19CIRR217 pKa = 11.84ITGEE221 pKa = 3.96LGPVSNVTVTGNFLLNGATSISAVDD246 pKa = 3.83GSGPGALSDD255 pKa = 3.54IAITGNYY262 pKa = 9.25LVGPKK267 pKa = 10.11YY268 pKa = 9.37YY269 pKa = 10.25TFFPGSMSGSTVSGNVIADD288 pKa = 3.57QTSLSDD294 pKa = 3.12AWAAYY299 pKa = 8.71QAAGVPTATLLASTDD314 pKa = 3.34GSAVSAASATAPTTLYY330 pKa = 11.04ADD332 pKa = 3.53VRR334 pKa = 11.84NTLMVGGPNEE344 pKa = 4.22TNFVSGYY351 pKa = 10.56ASQNMWAGSGADD363 pKa = 2.89IFTYY367 pKa = 10.6LATGDD372 pKa = 4.12SSPAAADD379 pKa = 3.75YY380 pKa = 8.43ITNFDD385 pKa = 3.92PAKK388 pKa = 10.68DD389 pKa = 3.95VIDD392 pKa = 4.57LSRR395 pKa = 11.84VDD397 pKa = 4.59ANPGGAPEE405 pKa = 4.11TFAFIGANAFDD416 pKa = 4.11GAGPEE421 pKa = 3.81IRR423 pKa = 11.84TQAEE427 pKa = 3.83ADD429 pKa = 3.38GDD431 pKa = 4.45TIVQVALAGDD441 pKa = 4.19TTPDD445 pKa = 3.2MQIILTSSVPLTAANFAVTAAQSQAAIANGADD477 pKa = 3.09MGFSVTSGWPLTEE490 pKa = 3.55TTYY493 pKa = 11.73ANVQGRR499 pKa = 11.84PYY501 pKa = 11.2SDD503 pKa = 3.21FTSFRR508 pKa = 11.84SGSYY512 pKa = 10.16LVADD516 pKa = 4.52ALDD519 pKa = 4.36LGGSSDD525 pKa = 4.76EE526 pKa = 4.15IDD528 pKa = 4.3LFGKK532 pKa = 9.72PGAAVTITRR541 pKa = 11.84GDD543 pKa = 3.41GAEE546 pKa = 4.19QITSAGKK553 pKa = 7.68TDD555 pKa = 3.37SLAFHH560 pKa = 6.97ANEE563 pKa = 4.46TIAAGAAPAGEE574 pKa = 4.33TFAIGPGSGNVTIEE588 pKa = 3.78GFAASGANADD598 pKa = 4.48RR599 pKa = 11.84LQLSASAFSGLQSGMTDD616 pKa = 3.31AEE618 pKa = 4.22KK619 pKa = 10.64LAAALGDD626 pKa = 3.46VSTASGGASFTDD638 pKa = 3.51SAGDD642 pKa = 3.36RR643 pKa = 11.84LTLVGVAPATLTANPGLVKK662 pKa = 10.16FVV664 pKa = 3.36

Molecular weight: 66.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A366FLI5|A0A366FLI5_9RHIZ UPF0114 protein DFR50_108120 OS=Roseiarcus fermentans OX=1473586 GN=DFR50_108120 PE=3 SV=1
MM1 pKa = 6.84TTRR4 pKa = 11.84TWTGILGVGALALAVSLALPATAQTTAPAPSAHH37 pKa = 6.81KK38 pKa = 11.02VPMHH42 pKa = 6.5PSALTLGSHH51 pKa = 5.85RR52 pKa = 11.84TRR54 pKa = 11.84PHH56 pKa = 5.26HH57 pKa = 6.1QGVGRR62 pKa = 11.84LGNTRR67 pKa = 11.84GRR69 pKa = 11.84QLHH72 pKa = 5.06TTPIHH77 pKa = 5.48GTMAGPRR84 pKa = 11.84II85 pKa = 3.98

Molecular weight: 8.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6190

0

6190

1936078

28

3024

312.8

33.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.584 ± 0.055

0.848 ± 0.009

5.687 ± 0.025

5.308 ± 0.032

3.734 ± 0.018

8.992 ± 0.039

1.82 ± 0.013

4.576 ± 0.021

2.796 ± 0.025

9.901 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.134 ± 0.015

2.306 ± 0.022

5.571 ± 0.031

2.597 ± 0.018

7.733 ± 0.049

5.289 ± 0.033

5.031 ± 0.04

7.646 ± 0.026

1.359 ± 0.014

2.086 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski