Gammaproteobacteria bacterium

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; unclassified Gammaproteobacteria

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3997 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q5VCC0|A0A4Q5VCC0_9GAMM Flavin reductase OS=Gammaproteobacteria bacterium OX=1913989 GN=EOO52_07800 PE=4 SV=1
LL1 pKa = 6.68GTDD4 pKa = 3.62GDD6 pKa = 4.06AGTTVTYY13 pKa = 11.01SLTDD17 pKa = 3.3NAGGRR22 pKa = 11.84FAINSTTGVITVANGSLLDD41 pKa = 3.89YY42 pKa = 11.01EE43 pKa = 5.05SATSHH48 pKa = 5.83NVTVLATSSDD58 pKa = 3.13GSTANRR64 pKa = 11.84AFSISVTNVNDD75 pKa = 3.76APVATAATYY84 pKa = 9.03TGNEE88 pKa = 3.84DD89 pKa = 3.31AAFITVNLAGTDD101 pKa = 3.29VDD103 pKa = 3.99GTIASLKK110 pKa = 10.18VSSLPTAAQGVLYY123 pKa = 10.53LADD126 pKa = 3.62GVTAVTTAMNLTPAQAAALKK146 pKa = 8.9FVPKK150 pKa = 10.5LNYY153 pKa = 10.23NGEE156 pKa = 3.83FDD158 pKa = 3.81IAFTATDD165 pKa = 3.11NSGAVSSPANAHH177 pKa = 4.66VVVNAVNDD185 pKa = 3.8APIANNDD192 pKa = 3.45PTASSSVGVGLLSQYY207 pKa = 9.86FAYY210 pKa = 10.83NEE212 pKa = 4.22GVNGGNLTSIAQMNAFISGRR232 pKa = 11.84TPDD235 pKa = 3.2ATFVAKK241 pKa = 9.63TFNYY245 pKa = 10.4GSDD248 pKa = 3.16NLFSNDD254 pKa = 3.5LGRR257 pKa = 11.84GTNLQSFLGSDD268 pKa = 3.59AASLSTDD275 pKa = 3.79PGDD278 pKa = 3.68STDD281 pKa = 4.6AIIRR285 pKa = 11.84MYY287 pKa = 10.96GAVEE291 pKa = 4.2LAAGTYY297 pKa = 8.95NFQVRR302 pKa = 11.84GDD304 pKa = 3.96DD305 pKa = 4.06GYY307 pKa = 11.28QIKK310 pKa = 10.62VDD312 pKa = 3.86GKK314 pKa = 10.41VVALVDD320 pKa = 5.48AIQSPTGTVHH330 pKa = 6.8SQFTLADD337 pKa = 3.88SGLHH341 pKa = 5.87TIEE344 pKa = 4.46ILYY347 pKa = 9.34WDD349 pKa = 3.64QGGQAVFKK357 pKa = 10.98IEE359 pKa = 4.57LSDD362 pKa = 3.91NNGVSYY368 pKa = 11.27NLLSSKK374 pKa = 8.51PTSYY378 pKa = 11.07NAVYY382 pKa = 10.54SLNEE386 pKa = 3.81DD387 pKa = 4.4TIWSVPVSTLLANDD401 pKa = 3.69TDD403 pKa = 3.86VDD405 pKa = 4.09GDD407 pKa = 3.88TLSIVSVQNALHH419 pKa = 5.78GTVQIVGSNVVFTPEE434 pKa = 3.78ANYY437 pKa = 10.84SGIASYY443 pKa = 9.69TYY445 pKa = 9.98TISDD449 pKa = 3.71GKK451 pKa = 11.05GGTSTAKK458 pKa = 10.53VNIDD462 pKa = 3.27IQPLNDD468 pKa = 3.8APLAVNDD475 pKa = 4.24NLTATEE481 pKa = 4.59DD482 pKa = 3.37ASITYY487 pKa = 8.04TAAQLLGNDD496 pKa = 3.61SDD498 pKa = 4.06VDD500 pKa = 3.92SSAFTISSVTSGTGGTAVLNANGTVTFTPTANFNGNATFTYY541 pKa = 10.29RR542 pKa = 11.84ATDD545 pKa = 3.99GSAISAPATVTVAVASVNDD564 pKa = 3.99APSGTSSSVGIIEE577 pKa = 4.72DD578 pKa = 3.44QSHH581 pKa = 6.14TLTVANFGFTDD592 pKa = 3.5TADD595 pKa = 3.87NNSLLAVRR603 pKa = 11.84ISTLPTSGTLYY614 pKa = 10.35LNNVAVTAGTYY625 pKa = 10.13ISVADD630 pKa = 3.89ISAGKK635 pKa = 10.18LIYY638 pKa = 10.49LPSNNDD644 pKa = 2.69SGTARR649 pKa = 11.84TFTFQVQDD657 pKa = 4.05DD658 pKa = 4.26GGTANGGVNLDD669 pKa = 3.64ATPNTLTFNIADD681 pKa = 3.76VPTTTDD687 pKa = 2.64KK688 pKa = 11.22TGNTNANTFAVLANSGNFSILDD710 pKa = 3.51SGGTDD715 pKa = 3.43SISVTTATGAVFNTLNFEE733 pKa = 4.42HH734 pKa = 6.62VGNNLEE740 pKa = 4.22FTQATAAGSANVSVINQYY758 pKa = 9.84VAANTIEE765 pKa = 4.17TLSFSSGGDD774 pKa = 3.27YY775 pKa = 11.25AGFQVGTAATTTYY788 pKa = 10.46TLNTTNTGTSGNDD801 pKa = 3.08IIVGTTADD809 pKa = 3.79DD810 pKa = 4.4TISGGGGAGRR820 pKa = 11.84DD821 pKa = 3.67MLFGGAGNDD830 pKa = 3.81TITAAGTGNHH840 pKa = 6.36LLVGEE845 pKa = 5.15LGNDD849 pKa = 3.66TLIGGAGSDD858 pKa = 3.29WLIGGKK864 pKa = 10.65GNDD867 pKa = 3.98SLTGGAGVDD876 pKa = 3.42TFVWQLADD884 pKa = 3.58KK885 pKa = 9.09GTTAAPAVDD894 pKa = 3.98TVNDD898 pKa = 3.64SDD900 pKa = 6.1GSDD903 pKa = 3.41KK904 pKa = 11.56LNLSDD909 pKa = 5.58LLQGEE914 pKa = 4.32NSGNLTNYY922 pKa = 10.42LHH924 pKa = 6.06FTSDD928 pKa = 3.17GTTTTVSISSTGAFDD943 pKa = 3.62GSNYY947 pKa = 8.18GTATDD952 pKa = 3.46QTIILTNVNLTGGDD966 pKa = 3.35AAIINLLKK974 pKa = 10.23TNNNLITDD982 pKa = 4.29

Molecular weight: 99.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q5VHC1|A0A4Q5VHC1_9GAMM tRNA-specific adenosine deaminase OS=Gammaproteobacteria bacterium OX=1913989 GN=tadA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTLKK11 pKa = 10.47RR12 pKa = 11.84ARR14 pKa = 11.84NHH16 pKa = 5.29GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.35NGRR28 pKa = 11.84LVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.0GRR39 pKa = 11.84KK40 pKa = 8.22EE41 pKa = 3.7LTVV44 pKa = 3.18

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3997

0

3997

1350151

18

3945

337.8

37.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.032 ± 0.043

0.956 ± 0.015

5.427 ± 0.031

5.792 ± 0.041

4.236 ± 0.024

6.892 ± 0.045

2.114 ± 0.019

6.446 ± 0.033

5.377 ± 0.033

10.102 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.18 ± 0.017

4.735 ± 0.037

4.141 ± 0.026

4.029 ± 0.027

4.806 ± 0.033

6.99 ± 0.051

5.592 ± 0.041

6.785 ± 0.034

1.309 ± 0.017

3.058 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski