Pelagibacter phage HTVC105P

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Pelagivirus; unclassified Pelagivirus

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y1NTB4|A0A4Y1NTB4_9CAUD Internal virion protein D OS=Pelagibacter phage HTVC105P OX=2283018 GN=P105_gp40 PE=4 SV=1
MM1 pKa = 7.47ANSFVRR7 pKa = 11.84YY8 pKa = 7.53TGNNSTTSYY17 pKa = 10.74AIPFSYY23 pKa = 10.38RR24 pKa = 11.84ATGDD28 pKa = 3.38LTVTLAGVATTAYY41 pKa = 8.61TLNAAGTTLTFNSAPAQDD59 pKa = 3.55VAIEE63 pKa = 4.07IRR65 pKa = 11.84RR66 pKa = 11.84KK67 pKa = 8.03TSQTTRR73 pKa = 11.84LTDD76 pKa = 3.51YY77 pKa = 11.47ADD79 pKa = 3.63GSVLTEE85 pKa = 4.11NDD87 pKa = 3.84LDD89 pKa = 4.08TDD91 pKa = 4.05STQAFYY97 pKa = 10.18MGQEE101 pKa = 4.74AIDD104 pKa = 4.3DD105 pKa = 4.41ANDD108 pKa = 3.41VIKK111 pKa = 10.53PSNTNFQWDD120 pKa = 3.85ANNKK124 pKa = 9.87RR125 pKa = 11.84IINVANPTSNQDD137 pKa = 3.03VATKK141 pKa = 10.18HH142 pKa = 5.5YY143 pKa = 11.03LEE145 pKa = 4.5NTWLSASDD153 pKa = 3.23KK154 pKa = 10.53TAITTVNSNIANINTVNSNITNINSAVSNATNINTVATNISSVNTVAADD203 pKa = 3.29ITKK206 pKa = 10.39VIAVANDD213 pKa = 3.2LAEE216 pKa = 4.23AVSEE220 pKa = 4.31IEE222 pKa = 4.66TVADD226 pKa = 4.16DD227 pKa = 4.47LNEE230 pKa = 4.06TSSEE234 pKa = 4.1IEE236 pKa = 3.98VVGANITNVNTVGGISANVTTVAGVSANVTTVAGISANVTTVAGISGDD284 pKa = 3.56VTAVANISSDD294 pKa = 3.32VAAVEE299 pKa = 4.2NIKK302 pKa = 10.12TNVTTVAGIASDD314 pKa = 3.68VTAVANISSDD324 pKa = 3.32VAAVEE329 pKa = 4.42NIAANVTTVAGNNANITTVAGANSNITTVAGAITNVNNVGGAITNVNNVGGSIANVNTVATNLSGVNSFAEE400 pKa = 4.56RR401 pKa = 11.84YY402 pKa = 8.58RR403 pKa = 11.84VSSSAPTSSNDD414 pKa = 3.48IGDD417 pKa = 4.59LYY419 pKa = 11.21FDD421 pKa = 3.67STANEE426 pKa = 3.89LKK428 pKa = 10.52VYY430 pKa = 10.44KK431 pKa = 10.46SSGWAAAGSTVNGTSARR448 pKa = 11.84FNYY451 pKa = 8.0TATANQTTFTGADD464 pKa = 3.17TAGNTLAYY472 pKa = 9.9DD473 pKa = 3.67AGYY476 pKa = 11.18ADD478 pKa = 4.22VYY480 pKa = 11.21LNGVRR485 pKa = 11.84LSGADD490 pKa = 3.1ITITSGTSVVLATGAAVGDD509 pKa = 3.92ILDD512 pKa = 3.71VVAYY516 pKa = 7.7GTFNVASVNAGNIDD530 pKa = 3.64AGTLNSARR538 pKa = 11.84LPTVPVSKK546 pKa = 10.85GGTNLTALGTAGQVIKK562 pKa = 11.12VNSGANALEE571 pKa = 4.13YY572 pKa = 11.19VNGSITINGSAVSLGGSVTVGEE594 pKa = 4.65TKK596 pKa = 9.07PTATGCTPSTITNSATNVVIAGTNFTSIPQVWAINTATGIWYY638 pKa = 8.88LANSVTYY645 pKa = 9.82TSATSITANFTLTVDD660 pKa = 3.32AQYY663 pKa = 10.98KK664 pKa = 9.83IRR666 pKa = 11.84VEE668 pKa = 4.11NPDD671 pKa = 3.18GNATLSTNNILTVSDD686 pKa = 3.85EE687 pKa = 4.54PTWSTSSGSLGSVAGNFSGTVATLSASSDD716 pKa = 3.49SAITYY721 pKa = 10.31SEE723 pKa = 4.18TTNVLTNASQANCALNSSTGAITTSDD749 pKa = 3.62FGGSSTTATTYY760 pKa = 11.13NFTIRR765 pKa = 11.84ATDD768 pKa = 3.74AEE770 pKa = 4.56SQTTDD775 pKa = 2.6RR776 pKa = 11.84TFSLTSSFGATGGGQFNN793 pKa = 4.04

Molecular weight: 80.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y1NUL1|A0A4Y1NUL1_9CAUD Uncharacterized protein OS=Pelagibacter phage HTVC105P OX=2283018 GN=P105_gp37 PE=4 SV=1
MM1 pKa = 7.84TYY3 pKa = 10.24TDD5 pKa = 4.22LFKK8 pKa = 11.29DD9 pKa = 3.12VDD11 pKa = 3.64KK12 pKa = 10.8PRR14 pKa = 11.84KK15 pKa = 8.25KK16 pKa = 9.96RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84KK20 pKa = 9.48RR21 pKa = 11.84KK22 pKa = 9.06EE23 pKa = 3.57KK24 pKa = 9.49QTVLWTVYY32 pKa = 8.84HH33 pKa = 6.8TILAVEE39 pKa = 4.29LLIIIIIEE47 pKa = 4.68GIEE50 pKa = 3.91LLRR53 pKa = 5.58

Molecular weight: 6.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

13116

39

1250

234.2

26.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.548 ± 0.493

0.915 ± 0.164

5.756 ± 0.202

6.862 ± 0.552

3.644 ± 0.211

6.435 ± 0.381

1.799 ± 0.217

6.496 ± 0.235

8.783 ± 0.638

7.96 ± 0.416

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.463 ± 0.23

5.833 ± 0.478

2.867 ± 0.215

3.614 ± 0.256

3.835 ± 0.249

6.862 ± 0.377

7.472 ± 0.713

5.932 ± 0.31

1.296 ± 0.118

3.629 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski