Gordonia phage Untouchable

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Deejayvirinae; Kenoshavirus; Gordonia virus Untouchable

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649VAA3|A0A649VAA3_9CAUD Uncharacterized protein OS=Gordonia phage Untouchable OX=2656542 GN=14 PE=4 SV=1
MM1 pKa = 7.61EE2 pKa = 5.69EE3 pKa = 4.39YY4 pKa = 10.12MLSTADD10 pKa = 3.53NPFNPFTQWEE20 pKa = 3.82QWYY23 pKa = 10.2AFDD26 pKa = 5.09ARR28 pKa = 11.84EE29 pKa = 4.71GYY31 pKa = 7.59HH32 pKa = 5.46TPAYY36 pKa = 9.3LARR39 pKa = 11.84VVRR42 pKa = 11.84TSSEE46 pKa = 3.81LSEE49 pKa = 4.44ADD51 pKa = 4.08QIVALNDD58 pKa = 4.91GIDD61 pKa = 4.34EE62 pKa = 4.08ILQYY66 pKa = 11.46NLTGNYY72 pKa = 9.33IKK74 pKa = 9.8VTRR77 pKa = 11.84PPVVATT83 pKa = 4.1

Molecular weight: 9.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649V9S5|A0A649V9S5_9CAUD DNA-directed DNA polymerase OS=Gordonia phage Untouchable OX=2656542 GN=63 PE=4 SV=1
MM1 pKa = 7.04VHH3 pKa = 6.64VKK5 pKa = 10.06PKK7 pKa = 9.55WYY9 pKa = 7.91RR10 pKa = 11.84TKK12 pKa = 10.09PWDD15 pKa = 3.36RR16 pKa = 11.84HH17 pKa = 3.91GLILVTAGVIYY28 pKa = 10.36AAIGFMFTLQPATQLRR44 pKa = 11.84SEE46 pKa = 4.13NLKK49 pKa = 10.44LALSIMPYY57 pKa = 10.05FGWGVGFIVVGLFTVVTSRR76 pKa = 11.84WPLAPKK82 pKa = 10.34SLGYY86 pKa = 8.71STLTGWTVAWAGFHH100 pKa = 6.41IVGGDD105 pKa = 3.4GCC107 pKa = 4.5

Molecular weight: 11.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

93

0

93

19295

30

1703

207.5

22.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.728 ± 0.559

0.653 ± 0.119

6.162 ± 0.342

6.846 ± 0.273

3.498 ± 0.227

7.489 ± 0.389

1.985 ± 0.214

5.779 ± 0.286

5.95 ± 0.321

8.064 ± 0.328

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.804 ± 0.141

4.353 ± 0.16

4.245 ± 0.269

3.529 ± 0.137

5.017 ± 0.281

6.437 ± 0.176

6.525 ± 0.269

7.401 ± 0.244

1.337 ± 0.129

3.198 ± 0.261
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski