Roseburia sp. 831b

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Roseburia; unclassified Roseburia

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2925 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q9J6M3|A0A1Q9J6M3_9FIRM Uncharacterized protein OS=Roseburia sp. 831b OX=1261635 GN=BIV16_04395 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 10.33KK3 pKa = 10.34KK4 pKa = 10.01IVSVLLCVSMVATMAAGCGSSGDD27 pKa = 3.93DD28 pKa = 3.38TTTTDD33 pKa = 3.34SNSASGTTEE42 pKa = 3.71SADD45 pKa = 3.74ASGSDD50 pKa = 3.4AATGGLAYY58 pKa = 9.75TGDD61 pKa = 4.11LEE63 pKa = 4.46IMHH66 pKa = 7.06FSTEE70 pKa = 4.18EE71 pKa = 3.74EE72 pKa = 4.27SQGNGGSDD80 pKa = 3.47GMRR83 pKa = 11.84TVLQQWADD91 pKa = 3.36NNTGINLTQTVLANADD107 pKa = 3.94YY108 pKa = 8.21KK109 pKa = 10.45TQIQTLANADD119 pKa = 4.08DD120 pKa = 4.74LPDD123 pKa = 3.31VFLIQGMNVKK133 pKa = 9.21QWAEE137 pKa = 3.54QGLILDD143 pKa = 3.8LTDD146 pKa = 4.26YY147 pKa = 10.6IKK149 pKa = 10.78SSPYY153 pKa = 9.58SADD156 pKa = 3.24YY157 pKa = 10.66NEE159 pKa = 5.62SYY161 pKa = 8.02FTPFKK166 pKa = 11.1DD167 pKa = 3.31EE168 pKa = 4.41AGNIYY173 pKa = 10.08GFPALTGGTCTVVVYY188 pKa = 10.29DD189 pKa = 3.98SKK191 pKa = 11.49AWADD195 pKa = 3.26AGYY198 pKa = 8.42DD199 pKa = 3.64TFPSTWEE206 pKa = 4.06DD207 pKa = 3.38VEE209 pKa = 4.29AAIPKK214 pKa = 9.33LQEE217 pKa = 3.63AGYY220 pKa = 10.25QEE222 pKa = 5.63AIAFGNQGQWNMNSDD237 pKa = 4.33FLSTLGDD244 pKa = 3.59RR245 pKa = 11.84YY246 pKa = 8.88TGKK249 pKa = 10.47DD250 pKa = 2.97WFQSMIDD257 pKa = 3.41KK258 pKa = 10.65KK259 pKa = 11.08GAAFTDD265 pKa = 3.97DD266 pKa = 3.89EE267 pKa = 4.73FVAALTEE274 pKa = 4.2TQRR277 pKa = 11.84LFKK280 pKa = 10.63DD281 pKa = 3.09TDD283 pKa = 3.25IFNKK287 pKa = 10.24DD288 pKa = 3.04FNAITNEE295 pKa = 3.97DD296 pKa = 3.12ARR298 pKa = 11.84EE299 pKa = 3.89YY300 pKa = 11.28YY301 pKa = 9.96IGQDD305 pKa = 2.77AAAFIGGNWDD315 pKa = 2.82VSYY318 pKa = 10.78IQASLEE324 pKa = 4.08GTDD327 pKa = 5.66LYY329 pKa = 10.99DD330 pKa = 3.36TTKK333 pKa = 10.5FAVLPQPKK341 pKa = 9.28DD342 pKa = 3.28AKK344 pKa = 10.89YY345 pKa = 10.81DD346 pKa = 3.74ADD348 pKa = 3.82SQNIGLGYY356 pKa = 10.66AVAINSKK363 pKa = 10.42LADD366 pKa = 4.69DD367 pKa = 4.69PDD369 pKa = 4.17KK370 pKa = 11.19LAAAIDD376 pKa = 3.62LAEE379 pKa = 4.78YY380 pKa = 8.92ITGPEE385 pKa = 4.02FANFVCDD392 pKa = 4.22KK393 pKa = 9.99YY394 pKa = 11.74ALGGLTKK401 pKa = 10.72VNDD404 pKa = 3.26VDD406 pKa = 5.22LSKK409 pKa = 10.81FDD411 pKa = 4.32QFTQDD416 pKa = 3.53FYY418 pKa = 11.63NYY420 pKa = 9.96SYY422 pKa = 11.84VDD424 pKa = 3.43TTPCEE429 pKa = 3.97IYY431 pKa = 10.54DD432 pKa = 4.04SYY434 pKa = 11.5LSSDD438 pKa = 2.9VWSVVNTDD446 pKa = 3.43LQSMVNGDD454 pKa = 3.57MTPEE458 pKa = 3.85EE459 pKa = 4.27VAKK462 pKa = 10.4DD463 pKa = 3.41AQEE466 pKa = 4.4AYY468 pKa = 8.46EE469 pKa = 4.14ANYY472 pKa = 10.55

Molecular weight: 51.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q9J3C3|A0A1Q9J3C3_9FIRM 23S rRNA (Guanosine(2251)-2'-O)-methyltransferase RlmB OS=Roseburia sp. 831b OX=1261635 GN=BIV16_07035 PE=4 SV=1
MM1 pKa = 7.64HH2 pKa = 7.44KK3 pKa = 10.88GKK5 pKa = 8.15MTFQPKK11 pKa = 9.0NRR13 pKa = 11.84QRR15 pKa = 11.84SKK17 pKa = 8.76VHH19 pKa = 5.91GFRR22 pKa = 11.84SRR24 pKa = 11.84MSTAGGRR31 pKa = 11.84KK32 pKa = 8.62VLAARR37 pKa = 11.84RR38 pKa = 11.84LKK40 pKa = 10.61GRR42 pKa = 11.84KK43 pKa = 8.87KK44 pKa = 10.59LSAA47 pKa = 3.95

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2925

0

2925

949512

37

2533

324.6

36.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.276 ± 0.043

1.459 ± 0.018

5.781 ± 0.041

8.057 ± 0.055

4.161 ± 0.033

6.668 ± 0.041

1.709 ± 0.019

7.308 ± 0.047

7.351 ± 0.04

8.731 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.102 ± 0.023

4.597 ± 0.031

2.989 ± 0.026

3.469 ± 0.028

3.86 ± 0.032

5.773 ± 0.042

5.569 ± 0.044

7.004 ± 0.036

0.87 ± 0.019

4.266 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski