Gastropod associated circular ssDNA virus

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 5.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1RKT4|M1RKT4_9VIRU Putative capsid protein OS=Gastropod associated circular ssDNA virus OX=1296573 PE=4 SV=1
MM1 pKa = 7.37NMAKK5 pKa = 8.92RR6 pKa = 11.84TNKK9 pKa = 8.16THH11 pKa = 6.14AQRR14 pKa = 11.84AFQIDD19 pKa = 4.16TIGGSGQKK27 pKa = 9.76QLIQLDD33 pKa = 3.71ACLSKK38 pKa = 10.91LNHH41 pKa = 6.1RR42 pKa = 11.84LYY44 pKa = 10.74RR45 pKa = 11.84QGRR48 pKa = 11.84MYY50 pKa = 10.25RR51 pKa = 11.84AKK53 pKa = 10.76VNLPASQMPVVNSEE67 pKa = 4.51PFPQFPQTKK76 pKa = 9.71GIEE79 pKa = 3.98VWALMDD85 pKa = 3.43TWYY88 pKa = 10.73VHH90 pKa = 6.82AMWKK94 pKa = 7.54QAKK97 pKa = 9.06RR98 pKa = 11.84AYY100 pKa = 10.23DD101 pKa = 3.37RR102 pKa = 11.84ALSDD106 pKa = 3.43EE107 pKa = 4.48KK108 pKa = 11.06KK109 pKa = 10.79ALAQQNIARR118 pKa = 11.84WRR120 pKa = 11.84DD121 pKa = 3.25FRR123 pKa = 11.84VDD125 pKa = 3.12SGQTGLIGPAGPEE138 pKa = 3.98GLSPLGYY145 pKa = 10.46KK146 pKa = 9.19GTTTFADD153 pKa = 3.7FDD155 pKa = 4.01KK156 pKa = 11.55GMFAYY161 pKa = 9.15STVEE165 pKa = 3.98NLDD168 pKa = 3.33NGNNMTFTFGDD179 pKa = 3.31GTMTQFSMPEE189 pKa = 3.75QLSLSRR195 pKa = 11.84NEE197 pKa = 4.16SASPEE202 pKa = 4.18TVITQMPYY210 pKa = 9.74NQLNAEE216 pKa = 4.53GEE218 pKa = 4.17DD219 pKa = 3.32ADD221 pKa = 4.2YY222 pKa = 11.6QEE224 pKa = 4.86LQANGSEE231 pKa = 4.22PPYY234 pKa = 10.81DD235 pKa = 3.96ADD237 pKa = 5.25AFPKK241 pKa = 10.29YY242 pKa = 9.84IWTKK246 pKa = 10.65VGTLGPFGTAIGAGGTYY263 pKa = 10.26DD264 pKa = 3.47KK265 pKa = 11.21QIVYY269 pKa = 10.48QSTTGYY275 pKa = 10.59FDD277 pKa = 4.28APCGMVLIEE286 pKa = 4.08NHH288 pKa = 6.0LTDD291 pKa = 4.06GDD293 pKa = 4.07ATVTTTIDD301 pKa = 3.4VEE303 pKa = 4.61VQAGNYY309 pKa = 9.48LGVHH313 pKa = 6.46ASVMGG318 pKa = 3.67

Molecular weight: 35.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1RKT4|M1RKT4_9VIRU Putative capsid protein OS=Gastropod associated circular ssDNA virus OX=1296573 PE=4 SV=1
MM1 pKa = 6.98AQRR4 pKa = 11.84VRR6 pKa = 11.84RR7 pKa = 11.84YY8 pKa = 10.25CLTLNNPTPEE18 pKa = 4.22EE19 pKa = 4.04CHH21 pKa = 6.42HH22 pKa = 6.69LLTLRR27 pKa = 11.84EE28 pKa = 4.33SVFKK32 pKa = 10.81RR33 pKa = 11.84GFFASEE39 pKa = 4.03VGKK42 pKa = 10.73KK43 pKa = 7.82GTPHH47 pKa = 6.08LQGFVHH53 pKa = 6.87LKK55 pKa = 8.94NAKK58 pKa = 8.4TLTGLKK64 pKa = 10.03KK65 pKa = 10.45FLGSDD70 pKa = 3.77RR71 pKa = 11.84YY72 pKa = 9.85HH73 pKa = 6.47VKK75 pKa = 9.84QANGTDD81 pKa = 3.71YY82 pKa = 11.48EE83 pKa = 4.08NWQYY87 pKa = 11.3IGLLNEE93 pKa = 4.83GKK95 pKa = 10.21AQGDD99 pKa = 4.73LIVQWGEE106 pKa = 3.99TPTEE110 pKa = 4.2EE111 pKa = 4.87GDD113 pKa = 3.26PDD115 pKa = 3.32AWDD118 pKa = 4.73SILEE122 pKa = 4.16MIEE125 pKa = 4.29GGFNNRR131 pKa = 11.84DD132 pKa = 3.35IVRR135 pKa = 11.84KK136 pKa = 8.01WPSIAIRR143 pKa = 11.84CQSAIDD149 pKa = 3.92RR150 pKa = 11.84YY151 pKa = 9.52RR152 pKa = 11.84VEE154 pKa = 4.39YY155 pKa = 10.33EE156 pKa = 3.49WGEE159 pKa = 3.93CRR161 pKa = 11.84AWRR164 pKa = 11.84DD165 pKa = 3.32VEE167 pKa = 4.07VEE169 pKa = 4.3YY170 pKa = 10.77IGGPTGTGKK179 pKa = 8.23TRR181 pKa = 11.84GVLYY185 pKa = 10.55HH186 pKa = 7.14ADD188 pKa = 3.1GRR190 pKa = 11.84VNTDD194 pKa = 2.31VYY196 pKa = 11.0RR197 pKa = 11.84CTNGKK202 pKa = 9.14HH203 pKa = 6.37PFDD206 pKa = 4.9KK207 pKa = 11.12YY208 pKa = 10.87DD209 pKa = 3.63GEE211 pKa = 4.34GTIVFEE217 pKa = 4.61EE218 pKa = 4.53FRR220 pKa = 11.84SQYY223 pKa = 7.73TCRR226 pKa = 11.84DD227 pKa = 3.34MLNWIDD233 pKa = 3.89GHH235 pKa = 6.51PLLLPARR242 pKa = 11.84YY243 pKa = 9.19ADD245 pKa = 3.78RR246 pKa = 11.84MAKK249 pKa = 7.73FTKK252 pKa = 10.51VIILSNWRR260 pKa = 11.84FAEE263 pKa = 4.02QYY265 pKa = 8.51RR266 pKa = 11.84TVAEE270 pKa = 4.91DD271 pKa = 3.33SPEE274 pKa = 4.11TYY276 pKa = 10.11KK277 pKa = 10.99AWLRR281 pKa = 11.84RR282 pKa = 11.84VGTITEE288 pKa = 3.9QWVV291 pKa = 3.03

Molecular weight: 33.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

609

291

318

304.5

34.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.389 ± 1.242

1.314 ± 0.491

6.076 ± 0.153

6.076 ± 1.201

3.941 ± 0.106

9.195 ± 0.171

2.135 ± 0.404

4.269 ± 0.356

5.09 ± 0.268

6.897 ± 0.21

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.791 ± 0.931

4.598 ± 0.311

4.433 ± 0.429

5.255 ± 1.194

5.911 ± 1.534

4.105 ± 0.665

7.882 ± 0.437

5.583 ± 0.396

2.463 ± 0.639

4.598 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski