Gila monster-associated gemycircularvirus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; unclassified Gemycircularvirus

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4N3Z3|A0A2Z4N3Z3_9VIRU Capsid protein OS=Gila monster-associated gemycircularvirus OX=2249931 PE=4 SV=1
MM1 pKa = 7.6PRR3 pKa = 11.84FRR5 pKa = 11.84CRR7 pKa = 11.84ARR9 pKa = 11.84NFIITFPQVAEE20 pKa = 4.09NVQPRR25 pKa = 11.84FDD27 pKa = 4.86DD28 pKa = 3.72GTPFLEE34 pKa = 4.76RR35 pKa = 11.84VTSDD39 pKa = 3.66FGNPLCCRR47 pKa = 11.84LGRR50 pKa = 11.84EE51 pKa = 3.7RR52 pKa = 11.84HH53 pKa = 5.76ADD55 pKa = 3.38GGVHH59 pKa = 4.27YY60 pKa = 10.06HH61 pKa = 6.53VYY63 pKa = 10.77LGFDD67 pKa = 3.95KK68 pKa = 10.69IVSINSATAFDD79 pKa = 4.16YY80 pKa = 11.19FGAHH84 pKa = 6.18GNIKK88 pKa = 9.32SVRR91 pKa = 11.84RR92 pKa = 11.84TPHH95 pKa = 6.08KK96 pKa = 10.73VFDD99 pKa = 4.1YY100 pKa = 10.67VGKK103 pKa = 10.61DD104 pKa = 2.82GDD106 pKa = 3.35IRR108 pKa = 11.84LDD110 pKa = 3.41HH111 pKa = 6.83GEE113 pKa = 4.33PPAEE117 pKa = 4.09ASSRR121 pKa = 11.84DD122 pKa = 3.64GTDD125 pKa = 2.95SNKK128 pKa = 9.08WADD131 pKa = 4.41IISCDD136 pKa = 3.55TKK138 pKa = 11.11EE139 pKa = 4.29SFLSAVRR146 pKa = 11.84EE147 pKa = 3.89QAPRR151 pKa = 11.84DD152 pKa = 3.5WLLSNQRR159 pKa = 11.84ILEE162 pKa = 4.17YY163 pKa = 11.19ANLYY167 pKa = 9.47YY168 pKa = 10.01PEE170 pKa = 5.11EE171 pKa = 4.43KK172 pKa = 9.8PQYY175 pKa = 7.69EE176 pKa = 4.24APPITVEE183 pKa = 3.78RR184 pKa = 11.84EE185 pKa = 3.74RR186 pKa = 11.84YY187 pKa = 9.12PEE189 pKa = 3.64ISEE192 pKa = 4.33WEE194 pKa = 3.98NQAAIGDD201 pKa = 4.15CRR203 pKa = 11.84PEE205 pKa = 3.64RR206 pKa = 4.61

Molecular weight: 23.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4N3Z3|A0A2Z4N3Z3_9VIRU Capsid protein OS=Gila monster-associated gemycircularvirus OX=2249931 PE=4 SV=1
MM1 pKa = 7.64AFRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84SFRR9 pKa = 11.84RR10 pKa = 11.84TGRR13 pKa = 11.84SYY15 pKa = 10.99RR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84GNARR22 pKa = 11.84TSRR25 pKa = 11.84GRR27 pKa = 11.84TSGRR31 pKa = 11.84GGYY34 pKa = 7.81NRR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84YY39 pKa = 7.41TGRR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84KK45 pKa = 6.95MTSRR49 pKa = 11.84RR50 pKa = 11.84VRR52 pKa = 11.84DD53 pKa = 3.0IASRR57 pKa = 11.84KK58 pKa = 10.0KK59 pKa = 9.71YY60 pKa = 9.5DD61 pKa = 3.78TIFGATSNDD70 pKa = 3.49VQSTGQANIFAGTNYY85 pKa = 10.05FMWCPTWRR93 pKa = 11.84QRR95 pKa = 11.84RR96 pKa = 11.84TASTDD101 pKa = 2.79EE102 pKa = 4.09HH103 pKa = 7.51VRR105 pKa = 11.84ANQEE109 pKa = 3.49VFMRR113 pKa = 11.84GIKK116 pKa = 9.9DD117 pKa = 3.71RR118 pKa = 11.84IMVSATFSLIHH129 pKa = 6.51RR130 pKa = 11.84RR131 pKa = 11.84VCFWSHH137 pKa = 6.1RR138 pKa = 11.84RR139 pKa = 11.84IDD141 pKa = 3.56DD142 pKa = 3.43ARR144 pKa = 11.84PFVFDD149 pKa = 3.94NPDD152 pKa = 3.46VIDD155 pKa = 4.11APSYY159 pKa = 8.73QRR161 pKa = 11.84RR162 pKa = 11.84NLVALYY168 pKa = 9.61PNLDD172 pKa = 3.66EE173 pKa = 6.25EE174 pKa = 4.21IFEE177 pKa = 4.38YY178 pKa = 10.78LFKK181 pKa = 10.78GTVGFDD187 pKa = 3.3YY188 pKa = 11.33SEE190 pKa = 4.66DD191 pKa = 4.01SRR193 pKa = 11.84WDD195 pKa = 3.43TPLDD199 pKa = 3.53NKK201 pKa = 10.08RR202 pKa = 11.84VKK204 pKa = 10.23VVYY207 pKa = 10.04DD208 pKa = 3.35KK209 pKa = 11.31QYY211 pKa = 10.98TITPNYY217 pKa = 8.66AAPDD221 pKa = 3.72GAQFGKK227 pKa = 8.75TLTRR231 pKa = 11.84KK232 pKa = 9.16LWHH235 pKa = 6.6PINRR239 pKa = 11.84KK240 pKa = 8.33VLYY243 pKa = 10.66DD244 pKa = 4.14DD245 pKa = 5.44DD246 pKa = 5.45EE247 pKa = 5.3EE248 pKa = 5.35GADD251 pKa = 3.75VNGSGWSAQTPEE263 pKa = 4.28SLGNYY268 pKa = 8.41YY269 pKa = 10.94VLDD272 pKa = 3.7IFSTGQYY279 pKa = 9.92IEE281 pKa = 4.51GSNDD285 pKa = 3.24QVGTYY290 pKa = 8.5MPEE293 pKa = 3.8STTYY297 pKa = 8.23WHH299 pKa = 6.65EE300 pKa = 3.94ASS302 pKa = 3.92

Molecular weight: 35.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

837

206

329

279.0

32.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.332 ± 0.274

1.912 ± 0.552

7.766 ± 0.072

5.974 ± 0.923

5.376 ± 0.201

6.81 ± 0.215

2.509 ± 0.374

5.496 ± 0.537

4.182 ± 0.423

4.54 ± 0.4

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.434 ± 0.303

4.54 ± 0.189

5.376 ± 0.793

3.106 ± 0.091

10.036 ± 1.5

6.332 ± 0.416

5.376 ± 1.121

5.615 ± 0.083

1.912 ± 0.187

5.376 ± 0.321
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski