Lactuca saligna (Willowleaf lettuce)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; campanulids; Asterales; Asteraceae; Cichorioideae; Cichorieae; Lactucinae; Lactuca

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44179 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6S7PJR3|A0A6S7PJR3_LACSI Uncharacterized protein OS=Lactuca saligna OX=75948 GN=LSAL_LOCUS40659 PE=4 SV=1
MM1 pKa = 7.53EE2 pKa = 6.13CDD4 pKa = 3.11NSIVKK9 pKa = 9.38NVKK12 pKa = 9.95HH13 pKa = 6.11EE14 pKa = 4.26EE15 pKa = 4.09PTSEE19 pKa = 4.09VGTSGSLNSDD29 pKa = 3.54MLYY32 pKa = 10.61HH33 pKa = 7.91DD34 pKa = 6.19DD35 pKa = 6.27DD36 pKa = 7.51DD37 pKa = 7.62DD38 pKa = 7.63DD39 pKa = 7.41DD40 pKa = 7.29DD41 pKa = 7.55DD42 pKa = 6.85DD43 pKa = 7.14DD44 pKa = 6.23EE45 pKa = 6.49MMDD48 pKa = 3.36NWDD51 pKa = 5.13DD52 pKa = 3.8VDD54 pKa = 6.0DD55 pKa = 6.33DD56 pKa = 4.94FDD58 pKa = 5.93LDD60 pKa = 4.03YY61 pKa = 11.48EE62 pKa = 4.55GDD64 pKa = 3.83YY65 pKa = 10.55MFEE68 pKa = 4.03ILEE71 pKa = 4.49SDD73 pKa = 4.27DD74 pKa = 3.52EE75 pKa = 4.41SHH77 pKa = 6.57YY78 pKa = 11.36LSIQSRR84 pKa = 11.84FDD86 pKa = 4.07NINLPPGCWFSRR98 pKa = 11.84ATDD101 pKa = 3.86SIFVRR106 pKa = 11.84AYY108 pKa = 6.7EE109 pKa = 4.05TSVDD113 pKa = 3.84LLRR116 pKa = 11.84GVIVGPAGTPYY127 pKa = 10.57HH128 pKa = 7.45DD129 pKa = 4.13GLLYY133 pKa = 10.85SIFTFLLTTYY143 pKa = 7.06THH145 pKa = 6.68RR146 pKa = 11.84RR147 pKa = 11.84YY148 pKa = 8.65TKK150 pKa = 10.6LVDD153 pKa = 4.22FGG155 pKa = 3.96

Molecular weight: 17.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6S7N2C2|A0A6S7N2C2_LACSI Uncharacterized protein OS=Lactuca saligna OX=75948 GN=LSAL_LOCUS22773 PE=4 SV=1
MM1 pKa = 7.65SSTITTTIILLLFITLINIPKK22 pKa = 9.86SQSQSPKK29 pKa = 9.9FPCQPPHH36 pKa = 6.26HH37 pKa = 6.8NSYY40 pKa = 10.05PFCNTALPIPTRR52 pKa = 11.84AQSLLSLLSLSDD64 pKa = 3.56KK65 pKa = 10.58IKK67 pKa = 10.7RR68 pKa = 11.84LSNNDD73 pKa = 2.94TGIPRR78 pKa = 11.84LGIPPYY84 pKa = 9.06EE85 pKa = 3.87WWSEE89 pKa = 4.14SLHH92 pKa = 6.63GIASNGPRR100 pKa = 11.84CHH102 pKa = 6.09IRR104 pKa = 11.84NRR106 pKa = 11.84THH108 pKa = 6.88PRR110 pKa = 11.84RR111 pKa = 11.84HH112 pKa = 6.31RR113 pKa = 11.84LPTSHH118 pKa = 6.36SHH120 pKa = 7.3HH121 pKa = 6.96ILFQSDD127 pKa = 3.84VMVFNCFGYY136 pKa = 10.26RR137 pKa = 11.84RR138 pKa = 11.84RR139 pKa = 11.84SKK141 pKa = 11.45GNVQRR146 pKa = 11.84GSSRR150 pKa = 11.84VDD152 pKa = 3.52FLGSDD157 pKa = 3.86DD158 pKa = 3.73

Molecular weight: 17.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

41650

2529

44179

16484648

8

5465

373.1

41.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.708 ± 0.011

1.817 ± 0.006

5.575 ± 0.008

6.54 ± 0.015

4.341 ± 0.008

6.254 ± 0.011

2.554 ± 0.006

5.802 ± 0.009

6.534 ± 0.012

9.401 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.603 ± 0.004

4.845 ± 0.009

4.845 ± 0.013

3.565 ± 0.007

4.987 ± 0.009

8.659 ± 0.014

5.256 ± 0.008

6.522 ± 0.009

1.289 ± 0.004

2.893 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski