McMurdo Ice Shelf pond-associated circular DNA virus-6

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075LZ80|A0A075LZ80_9VIRU Uncharacterized protein OS=McMurdo Ice Shelf pond-associated circular DNA virus-6 OX=1521390 PE=4 SV=1
MM1 pKa = 8.12DD2 pKa = 4.04GQEE5 pKa = 3.79ACEE8 pKa = 4.15EE9 pKa = 4.31VLSSSGEE16 pKa = 4.1EE17 pKa = 3.82QDD19 pKa = 4.46SNPVDD24 pKa = 3.15VWDD27 pKa = 4.27EE28 pKa = 3.9VSGEE32 pKa = 4.25WKK34 pKa = 9.61HH35 pKa = 6.37HH36 pKa = 5.16PRR38 pKa = 11.84AEE40 pKa = 3.96KK41 pKa = 10.48RR42 pKa = 11.84PMGSDD47 pKa = 3.89EE48 pKa = 5.13EE49 pKa = 4.37ILDD52 pKa = 3.8NASKK56 pKa = 10.4FFQSFKK62 pKa = 10.47EE63 pKa = 3.8SLMCHH68 pKa = 5.6EE69 pKa = 5.14VWAQPDD75 pKa = 3.41LAEE78 pKa = 4.06YY79 pKa = 9.37FANFEE84 pKa = 4.06ISEE87 pKa = 4.27FTQISLCRR95 pKa = 11.84TYY97 pKa = 11.54ANYY100 pKa = 10.01LAQVMRR106 pKa = 11.84ARR108 pKa = 11.84NAGTPSEE115 pKa = 4.29GQSPPGGANSLRR127 pKa = 11.84VGHH130 pKa = 6.37TSTRR134 pKa = 11.84KK135 pKa = 9.25IKK137 pKa = 10.59RR138 pKa = 11.84KK139 pKa = 9.99LFF141 pKa = 3.81

Molecular weight: 15.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075LYS7|A0A075LYS7_9VIRU ATP-dependent helicase Rep OS=McMurdo Ice Shelf pond-associated circular DNA virus-6 OX=1521390 PE=3 SV=1
MM1 pKa = 7.44SYY3 pKa = 10.5KK4 pKa = 10.44RR5 pKa = 11.84KK6 pKa = 9.37LSKK9 pKa = 9.74SARR12 pKa = 11.84YY13 pKa = 8.95NRR15 pKa = 11.84RR16 pKa = 11.84PLKK19 pKa = 10.45VISVAQKK26 pKa = 11.02AKK28 pKa = 10.02LQRR31 pKa = 11.84ARR33 pKa = 11.84SGIPKK38 pKa = 10.0VIVAASDD45 pKa = 3.34PMMKK49 pKa = 9.94QLRR52 pKa = 11.84GLLAAKK58 pKa = 9.41TRR60 pKa = 11.84DD61 pKa = 3.4ALDD64 pKa = 3.34VYY66 pKa = 11.19RR67 pKa = 11.84NATAGGGVSRR77 pKa = 11.84VSLLTSSSPFTTAAAATGILDD98 pKa = 3.83ADD100 pKa = 3.97ADD102 pKa = 4.03EE103 pKa = 4.38VLINSVRR110 pKa = 11.84IAGYY114 pKa = 10.36HH115 pKa = 6.43DD116 pKa = 3.68NQFQADD122 pKa = 4.1TDD124 pKa = 4.03PVGNFPTFVRR134 pKa = 11.84VLVVWMTKK142 pKa = 9.98PLLIASAAGTLPTVTEE158 pKa = 4.18VLTAASIDD166 pKa = 3.5AMPIQSQQNGGRR178 pKa = 11.84FTILSDD184 pKa = 3.55RR185 pKa = 11.84RR186 pKa = 11.84WNLGLATTSASVSTTSAQSMRR207 pKa = 11.84QFFDD211 pKa = 3.12YY212 pKa = 9.87TIKK215 pKa = 10.56VGRR218 pKa = 11.84RR219 pKa = 11.84CKK221 pKa = 10.24FVTPAASGAATAGGHH236 pKa = 5.91YY237 pKa = 8.75GTGAATGQLSTGLLLMYY254 pKa = 10.88SMAVGPAGLNTNVNFATRR272 pKa = 11.84VNYY275 pKa = 9.49TGG277 pKa = 3.41

Molecular weight: 29.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

744

141

326

248.0

27.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.005 ± 2.131

2.151 ± 0.858

4.704 ± 0.168

4.57 ± 2.443

5.78 ± 1.344

7.392 ± 0.452

2.957 ± 1.084

3.36 ± 0.283

5.108 ± 0.392

7.527 ± 0.685

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.957 ± 0.063

3.629 ± 0.322

5.511 ± 0.828

4.301 ± 0.231

6.183 ± 0.307

8.065 ± 1.06

6.317 ± 1.572

6.048 ± 0.843

1.613 ± 0.397

2.823 ± 0.239
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski