Beihai tombus-like virus 5

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KG05|A0A1L3KG05_9VIRU Uncharacterized protein OS=Beihai tombus-like virus 5 OX=1922726 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 9.59MYY4 pKa = 10.67ARR6 pKa = 11.84QVCRR10 pKa = 11.84GDD12 pKa = 3.3LGAITTVSFDD22 pKa = 3.21EE23 pKa = 4.57AFRR26 pKa = 11.84NFPPAKK32 pKa = 7.32RR33 pKa = 11.84QKK35 pKa = 10.0YY36 pKa = 8.38AEE38 pKa = 4.33SYY40 pKa = 10.3EE41 pKa = 4.53KK42 pKa = 10.34IGSQPDD48 pKa = 3.58MAKK51 pKa = 8.66GHH53 pKa = 6.22CMAFVKK59 pKa = 10.06YY60 pKa = 9.92EE61 pKa = 4.41RR62 pKa = 11.84LAEE65 pKa = 4.0WDD67 pKa = 3.44KK68 pKa = 11.14DD69 pKa = 3.05PRR71 pKa = 11.84MIQFRR76 pKa = 11.84NAGYY80 pKa = 9.86GAALACMLKK89 pKa = 10.24PIEE92 pKa = 3.93HH93 pKa = 7.25RR94 pKa = 11.84IYY96 pKa = 10.97KK97 pKa = 10.36LRR99 pKa = 11.84DD100 pKa = 3.56SKK102 pKa = 11.61SGLPLVAKK110 pKa = 10.2CSNAHH115 pKa = 5.42EE116 pKa = 4.16RR117 pKa = 11.84AEE119 pKa = 3.83ILRR122 pKa = 11.84EE123 pKa = 3.36KK124 pKa = 9.84WNRR127 pKa = 11.84FEE129 pKa = 4.87NPVCISYY136 pKa = 10.66DD137 pKa = 3.46CSRR140 pKa = 11.84WDD142 pKa = 3.09AHH144 pKa = 6.42VKK146 pKa = 10.11VEE148 pKa = 4.03HH149 pKa = 6.92LEE151 pKa = 4.29LEE153 pKa = 4.19HH154 pKa = 6.86EE155 pKa = 4.99FYY157 pKa = 11.62SMCNPDD163 pKa = 3.67PQLPTLLQKK172 pKa = 9.11QLRR175 pKa = 11.84NSVFTANGLFYY186 pKa = 10.51QVLGGRR192 pKa = 11.84MSGDD196 pKa = 3.45MNTSSGNVALMLFFMTAATKK216 pKa = 10.02GFRR219 pKa = 11.84RR220 pKa = 11.84EE221 pKa = 4.04IYY223 pKa = 10.38DD224 pKa = 4.65DD225 pKa = 5.05GDD227 pKa = 3.78DD228 pKa = 3.6CVVIVEE234 pKa = 4.84AEE236 pKa = 4.1DD237 pKa = 3.59EE238 pKa = 4.44EE239 pKa = 5.17KK240 pKa = 10.54ISEE243 pKa = 4.47RR244 pKa = 11.84VLEE247 pKa = 4.34WFTQAGFVIRR257 pKa = 11.84VDD259 pKa = 3.22AVARR263 pKa = 11.84QFEE266 pKa = 4.81EE267 pKa = 5.01IMFCSCHH274 pKa = 5.94PVWDD278 pKa = 4.0GQIYY282 pKa = 10.87KK283 pKa = 9.1MVRR286 pKa = 11.84DD287 pKa = 3.47WRR289 pKa = 11.84KK290 pKa = 9.02VVGFGISGAKK300 pKa = 8.78WMRR303 pKa = 11.84QGEE306 pKa = 4.55KK307 pKa = 10.78AMKK310 pKa = 9.69TYY312 pKa = 10.5IRR314 pKa = 11.84TNGVCEE320 pKa = 3.77MALTRR325 pKa = 11.84GVPVLQTYY333 pKa = 7.79AQRR336 pKa = 11.84LIQFGQGGKK345 pKa = 9.85LVEE348 pKa = 4.75DD349 pKa = 3.26AMEE352 pKa = 4.11VKK354 pKa = 10.33KK355 pKa = 10.61RR356 pKa = 11.84LRR358 pKa = 11.84LAMSNDD364 pKa = 3.2QVEE367 pKa = 4.53SLDD370 pKa = 4.93QIDD373 pKa = 3.85TTPRR377 pKa = 11.84DD378 pKa = 3.62ITWEE382 pKa = 3.99SRR384 pKa = 11.84EE385 pKa = 4.01SFAIAFNINPQQQIDD400 pKa = 3.88MEE402 pKa = 4.39QTISNWTFPQIEE414 pKa = 4.31QCVLPEE420 pKa = 3.8WRR422 pKa = 11.84TSS424 pKa = 3.06

Molecular weight: 48.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KG05|A0A1L3KG05_9VIRU Uncharacterized protein OS=Beihai tombus-like virus 5 OX=1922726 PE=4 SV=1
MM1 pKa = 7.25LRR3 pKa = 11.84EE4 pKa = 4.44HH5 pKa = 6.79APEE8 pKa = 4.33MGTIMYY14 pKa = 10.39HH15 pKa = 6.42NGDD18 pKa = 3.44NGLNRR23 pKa = 11.84VLAVVCTTRR32 pKa = 11.84PAMDD36 pKa = 4.28LAQRR40 pKa = 11.84LRR42 pKa = 11.84EE43 pKa = 3.84IDD45 pKa = 4.27LLQRR49 pKa = 11.84WDD51 pKa = 3.72GPVYY55 pKa = 10.46IVPQRR60 pKa = 11.84IGRR63 pKa = 11.84FSAPQFIEE71 pKa = 4.12TCRR74 pKa = 11.84VAMPQCVVARR84 pKa = 11.84FYY86 pKa = 10.69PPRR89 pKa = 11.84RR90 pKa = 11.84LQQEE94 pKa = 4.15VEE96 pKa = 4.03EE97 pKa = 4.81ANQNDD102 pKa = 4.19AGEE105 pKa = 4.46EE106 pKa = 3.79PAVAQVEE113 pKa = 4.31RR114 pKa = 11.84QEE116 pKa = 4.44PDD118 pKa = 3.31DD119 pKa = 4.72NEE121 pKa = 3.82VDD123 pKa = 3.73EE124 pKa = 4.85NMEE127 pKa = 4.85LGPDD131 pKa = 3.22HH132 pKa = 6.85HH133 pKa = 8.39VPVDD137 pKa = 3.44DD138 pKa = 3.82ATLTGRR144 pKa = 11.84LATWWLRR151 pKa = 11.84ITRR154 pKa = 11.84QLRR157 pKa = 11.84QPEE160 pKa = 4.33VPADD164 pKa = 3.74VRR166 pKa = 11.84WARR169 pKa = 11.84CGVFPIINLRR179 pKa = 11.84ATGSGLMLVGSALAAANVPRR199 pKa = 11.84LTWEE203 pKa = 3.89LSYY206 pKa = 11.21NYY208 pKa = 9.72PCEE211 pKa = 4.2LTSKK215 pKa = 11.02ALATFCNATNVTLDD229 pKa = 3.25NALVHH234 pKa = 6.52HH235 pKa = 7.14IKK237 pKa = 10.25PYY239 pKa = 8.98SHH241 pKa = 7.39PAFHH245 pKa = 6.57ATYY248 pKa = 8.32LTRR251 pKa = 11.84VIKK254 pKa = 10.19FVSKK258 pKa = 10.85NIPLLANTSIGWTMRR273 pKa = 11.84KK274 pKa = 9.02CRR276 pKa = 11.84CTGPWYY282 pKa = 10.07YY283 pKa = 10.0PKK285 pKa = 10.52PKK287 pKa = 9.77WSWLGPAVFVIGCGLLYY304 pKa = 10.32IGVRR308 pKa = 11.84AIVRR312 pKa = 11.84SDD314 pKa = 3.6PEE316 pKa = 5.0ADD318 pKa = 3.05AVARR322 pKa = 11.84QAAFMRR328 pKa = 11.84PRR330 pKa = 11.84NRR332 pKa = 11.84NTLTVIEE339 pKa = 4.6GRR341 pKa = 11.84LDD343 pKa = 2.84AWMQRR348 pKa = 11.84EE349 pKa = 4.13NVPIVYY355 pKa = 10.02RR356 pKa = 11.84AGVRR360 pKa = 11.84VASAVRR366 pKa = 11.84AMDD369 pKa = 3.41VSPEE373 pKa = 4.03EE374 pKa = 4.1EE375 pKa = 4.01HH376 pKa = 7.48LLTQVGGVQQNLRR389 pKa = 11.84NADD392 pKa = 3.27VSGWIGNVSQYY403 pKa = 10.98LRR405 pKa = 11.84PFLPFLGVGLGLVYY419 pKa = 9.87FRR421 pKa = 11.84PPQSVLVAGRR431 pKa = 11.84SSLYY435 pKa = 11.29AMGQEE440 pKa = 4.97LIRR443 pKa = 11.84PGLQPTLTGGLHH455 pKa = 5.93

Molecular weight: 50.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

879

424

455

439.5

49.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.419 ± 0.605

2.389 ± 0.306

4.778 ± 0.613

6.485 ± 0.901

3.754 ± 0.832

6.485 ± 0.409

1.934 ± 0.197

4.778 ± 0.449

3.527 ± 1.481

8.191 ± 1.266

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.527 ± 0.663

4.096 ± 0.387

5.688 ± 1.329

5.119 ± 0.376

8.077 ± 0.532

4.437 ± 0.522

4.778 ± 0.37

8.077 ± 1.023

2.389 ± 0.021

3.072 ± 0.004
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski