Amino acid dipepetide frequency for Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.458AlaAla: 10.458 ± 0.155	0.614AlaCys: 0.614 ± 0.036	3.053AlaAsp: 3.053 ± 0.068	8.799AlaGlu: 8.799 ± 0.128	5.806AlaPhe: 5.806 ± 0.101	8.467AlaGly: 8.467 ± 0.122	2.453AlaHis: 2.453 ± 0.066	3.214AlaIle: 3.214 ± 0.085	4.082AlaLys: 4.082 ± 0.079	22.928AlaLeu: 22.928 ± 0.286	2.083AlaMet: 2.083 ± 0.059	1.389AlaAsn: 1.389 ± 0.051	5.758AlaPro: 5.758 ± 0.109	4.118AlaGln: 4.118 ± 0.085	10.627AlaArg: 10.627 ± 0.144	3.982AlaSer: 3.982 ± 0.083	3.948AlaThr: 3.948 ± 0.084	7.844AlaVal: 7.844 ± 0.119	2.152AlaTrp: 2.152 ± 0.067	3.988AlaTyr: 3.988 ± 0.085	0.002AlaXaa: 0.002 ± 0.001
Cys	0.383CysAla: 0.383 ± 0.028	0.041CysCys: 0.041 ± 0.008	0.149CysAsp: 0.149 ± 0.015	0.184CysGlu: 0.184 ± 0.017	0.125CysPhe: 0.125 ± 0.013	0.521CysGly: 0.521 ± 0.031	0.116CysHis: 0.116 ± 0.016	0.084CysIle: 0.084 ± 0.011	0.086CysLys: 0.086 ± 0.012	0.377CysLeu: 0.377 ± 0.021	0.03CysMet: 0.03 ± 0.007	0.117CysAsn: 0.117 ± 0.015	0.48CysPro: 0.48 ± 0.032	0.136CysGln: 0.136 ± 0.017	0.303CysArg: 0.303 ± 0.021	0.167CysSer: 0.167 ± 0.015	0.241CysThr: 0.241 ± 0.022	0.236CysVal: 0.236 ± 0.02	0.035CysTrp: 0.035 ± 0.009	0.116CysTyr: 0.116 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	2.99AspAla: 2.99 ± 0.062	0.127AspCys: 0.127 ± 0.016	0.898AspAsp: 0.898 ± 0.039	2.485AspGlu: 2.485 ± 0.072	1.499AspPhe: 1.499 ± 0.048	2.881AspGly: 2.881 ± 0.081	0.834AspHis: 0.834 ± 0.042	0.968AspIle: 0.968 ± 0.043	0.84AspLys: 0.84 ± 0.042	7.041AspLeu: 7.041 ± 0.1	0.438AspMet: 0.438 ± 0.024	0.456AspAsn: 0.456 ± 0.028	4.139AspPro: 4.139 ± 0.078	0.883AspGln: 0.883 ± 0.036	3.181AspArg: 3.181 ± 0.069	0.752AspSer: 0.752 ± 0.039	1.337AspThr: 1.337 ± 0.049	2.337AspVal: 2.337 ± 0.064	0.691AspTrp: 0.691 ± 0.033	1.176AspTyr: 1.176 ± 0.046	0.0AspXaa: 0.0 ± 0.0
Glu	16.207GluAla: 16.207 ± 0.209	0.157GluCys: 0.157 ± 0.016	3.432GluAsp: 3.432 ± 0.078	10.153GluGlu: 10.153 ± 0.164	1.587GluPhe: 1.587 ± 0.05	10.291GluGly: 10.291 ± 0.187	1.053GluHis: 1.053 ± 0.039	2.453GluIle: 2.453 ± 0.075	3.53GluLys: 3.53 ± 0.092	7.216GluLeu: 7.216 ± 0.113	1.276GluMet: 1.276 ± 0.044	1.521GluAsn: 1.521 ± 0.045	3.178GluPro: 3.178 ± 0.065	0.831GluGln: 0.831 ± 0.042	7.784GluArg: 7.784 ± 0.121	1.868GluSer: 1.868 ± 0.063	2.444GluThr: 2.444 ± 0.06	9.166GluVal: 9.166 ± 0.126	0.843GluTrp: 0.843 ± 0.037	1.181GluTyr: 1.181 ± 0.042	0.0GluXaa: 0.0 ± 0.0
Phe	4.17PheAla: 4.17 ± 0.092	0.163PheCys: 0.163 ± 0.015	1.259PheAsp: 1.259 ± 0.05	1.459PheGlu: 1.459 ± 0.049	1.756PhePhe: 1.756 ± 0.061	3.5PheGly: 3.5 ± 0.075	0.868PheHis: 0.868 ± 0.041	0.86PheIle: 0.86 ± 0.049	0.89PheLys: 0.89 ± 0.039	6.405PheLeu: 6.405 ± 0.124	0.482PheMet: 0.482 ± 0.03	0.61PheAsn: 0.61 ± 0.035	2.845PhePro: 2.845 ± 0.071	1.443PheGln: 1.443 ± 0.045	3.487PheArg: 3.487 ± 0.07	1.587PheSer: 1.587 ± 0.049	1.577PheThr: 1.577 ± 0.058	2.541PheVal: 2.541 ± 0.061	0.607PheTrp: 0.607 ± 0.032	1.217PheTyr: 1.217 ± 0.045	0.0PheXaa: 0.0 ± 0.0
Gly	8.802GlyAla: 8.802 ± 0.133	0.413GlyCys: 0.413 ± 0.024	3.018GlyAsp: 3.018 ± 0.067	8.202GlyGlu: 8.202 ± 0.155	4.109GlyPhe: 4.109 ± 0.085	8.232GlyGly: 8.232 ± 0.143	1.788GlyHis: 1.788 ± 0.053	2.764GlyIle: 2.764 ± 0.076	3.779GlyLys: 3.779 ± 0.089	14.02GlyLeu: 14.02 ± 0.206	1.901GlyMet: 1.901 ± 0.051	1.437GlyAsn: 1.437 ± 0.053	4.746GlyPro: 4.746 ± 0.093	2.25GlyGln: 2.25 ± 0.062	7.818GlyArg: 7.818 ± 0.145	3.381GlySer: 3.381 ± 0.073	3.563GlyThr: 3.563 ± 0.084	8.886GlyVal: 8.886 ± 0.121	1.184GlyTrp: 1.184 ± 0.04	2.967GlyTyr: 2.967 ± 0.074	0.002GlyXaa: 0.002 ± 0.001
His	1.904HisAla: 1.904 ± 0.059	0.083HisCys: 0.083 ± 0.011	0.568HisAsp: 0.568 ± 0.029	0.973HisGlu: 0.973 ± 0.04	0.652HisPhe: 0.652 ± 0.034	1.63HisGly: 1.63 ± 0.059	0.505HisHis: 0.505 ± 0.029	0.485HisIle: 0.485 ± 0.029	0.489HisLys: 0.489 ± 0.031	3.16HisLeu: 3.16 ± 0.076	0.28HisMet: 0.28 ± 0.024	0.307HisAsn: 0.307 ± 0.021	1.975HisPro: 1.975 ± 0.059	0.497HisGln: 0.497 ± 0.03	1.714HisArg: 1.714 ± 0.048	0.488HisSer: 0.488 ± 0.028	0.877HisThr: 0.877 ± 0.034	1.249HisVal: 1.249 ± 0.042	0.19HisTrp: 0.19 ± 0.017	0.578HisTyr: 0.578 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	3.268IleAla: 3.268 ± 0.083	0.117IleCys: 0.117 ± 0.012	0.941IleAsp: 0.941 ± 0.05	1.389IleGlu: 1.389 ± 0.056	0.78IlePhe: 0.78 ± 0.044	2.274IleGly: 2.274 ± 0.069	0.578IleHis: 0.578 ± 0.027	0.592IleIle: 0.592 ± 0.037	0.755IleLys: 0.755 ± 0.037	3.756IleLeu: 3.756 ± 0.088	0.309IleMet: 0.309 ± 0.025	0.499IleAsn: 0.499 ± 0.032	2.203IlePro: 2.203 ± 0.061	0.95IleGln: 0.95 ± 0.046	2.297IleArg: 2.297 ± 0.058	0.938IleSer: 0.938 ± 0.042	1.229IleThr: 1.229 ± 0.05	2.032IleVal: 2.032 ± 0.066	0.276IleTrp: 0.276 ± 0.022	0.771IleTyr: 0.771 ± 0.035	0.0IleXaa: 0.0 ± 0.0
Lys	5.857LysAla: 5.857 ± 0.107	0.095LysCys: 0.095 ± 0.013	1.854LysAsp: 1.854 ± 0.058	4.295LysGlu: 4.295 ± 0.079	0.461LysPhe: 0.461 ± 0.029	3.945LysGly: 3.945 ± 0.083	0.431LysHis: 0.431 ± 0.028	0.792LysIle: 0.792 ± 0.04	1.36LysLys: 1.36 ± 0.055	2.69LysLeu: 2.69 ± 0.068	0.381LysMet: 0.381 ± 0.026	0.712LysAsn: 0.712 ± 0.033	2.146LysPro: 2.146 ± 0.054	0.488LysGln: 0.488 ± 0.029	3.335LysArg: 3.335 ± 0.071	1.059LysSer: 1.059 ± 0.041	1.667LysThr: 1.667 ± 0.053	3.139LysVal: 3.139 ± 0.072	0.294LysTrp: 0.294 ± 0.022	0.575LysTyr: 0.575 ± 0.034	0.0LysXaa: 0.0 ± 0.0
Leu	18.466LeuAla: 18.466 ± 0.233	0.468LeuCys: 0.468 ± 0.026	5.573LeuAsp: 5.573 ± 0.093	13.468LeuGlu: 13.468 ± 0.18	5.473LeuPhe: 5.473 ± 0.11	13.617LeuGly: 13.617 ± 0.189	2.259LeuHis: 2.259 ± 0.061	2.771LeuIle: 2.771 ± 0.076	6.524LeuLys: 6.524 ± 0.102	20.958LeuLeu: 20.958 ± 0.336	1.874LeuMet: 1.874 ± 0.057	1.923LeuAsn: 1.923 ± 0.063	9.864LeuPro: 9.864 ± 0.162	2.926LeuGln: 2.926 ± 0.078	10.996LeuArg: 10.996 ± 0.155	6.924LeuSer: 6.924 ± 0.125	5.308LeuThr: 5.308 ± 0.095	10.029LeuVal: 10.029 ± 0.142	1.987LeuTrp: 1.987 ± 0.065	3.848LeuTyr: 3.848 ± 0.079	0.0LeuXaa: 0.0 ± 0.0
Met	2.08MetAla: 2.08 ± 0.061	0.056MetCys: 0.056 ± 0.01	0.77MetAsp: 0.77 ± 0.032	1.374MetGlu: 1.374 ± 0.046	0.294MetPhe: 0.294 ± 0.024	1.703MetGly: 1.703 ± 0.048	0.208MetHis: 0.208 ± 0.019	0.39MetIle: 0.39 ± 0.025	0.747MetLys: 0.747 ± 0.034	1.309MetLeu: 1.309 ± 0.05	0.25MetMet: 0.25 ± 0.022	0.348MetAsn: 0.348 ± 0.026	0.846MetPro: 0.846 ± 0.034	0.31MetGln: 0.31 ± 0.024	1.708MetArg: 1.708 ± 0.047	0.559MetSer: 0.559 ± 0.029	0.696MetThr: 0.696 ± 0.033	1.28MetVal: 1.28 ± 0.043	0.163MetTrp: 0.163 ± 0.017	0.258MetTyr: 0.258 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	1.788AsnAla: 1.788 ± 0.055	0.077AsnCys: 0.077 ± 0.012	0.467AsnAsp: 0.467 ± 0.028	0.66AsnGlu: 0.66 ± 0.036	0.571AsnPhe: 0.571 ± 0.031	1.057AsnGly: 1.057 ± 0.048	0.298AsnHis: 0.298 ± 0.022	0.486AsnIle: 0.486 ± 0.03	0.328AsnLys: 0.328 ± 0.023	2.645AsnLeu: 2.645 ± 0.07	0.185AsnMet: 0.185 ± 0.018	0.292AsnAsn: 0.292 ± 0.026	2.047AsnPro: 2.047 ± 0.057	0.447AsnGln: 0.447 ± 0.028	1.33AsnArg: 1.33 ± 0.05	0.435AsnSer: 0.435 ± 0.028	0.694AsnThr: 0.694 ± 0.04	1.11AsnVal: 1.11 ± 0.042	0.196AsnTrp: 0.196 ± 0.019	0.452AsnTyr: 0.452 ± 0.03	0.0AsnXaa: 0.0 ± 0.0
Pro	4.639ProAla: 4.639 ± 0.113	0.285ProCys: 0.285 ± 0.023	2.506ProAsp: 2.506 ± 0.064	7.794ProGlu: 7.794 ± 0.14	2.88ProPhe: 2.88 ± 0.062	6.532ProGly: 6.532 ± 0.126	1.368ProHis: 1.368 ± 0.046	1.709ProIle: 1.709 ± 0.05	2.976ProLys: 2.976 ± 0.067	9.132ProLeu: 9.132 ± 0.146	1.075ProMet: 1.075 ± 0.043	0.929ProAsn: 0.929 ± 0.036	4.919ProPro: 4.919 ± 0.126	1.761ProGln: 1.761 ± 0.06	4.476ProArg: 4.476 ± 0.092	2.416ProSer: 2.416 ± 0.061	2.414ProThr: 2.414 ± 0.065	4.636ProVal: 4.636 ± 0.089	1.295ProTrp: 1.295 ± 0.048	2.06ProTyr: 2.06 ± 0.058	0.0ProXaa: 0.0 ± 0.0
Gln	4.148GlnAla: 4.148 ± 0.097	0.054GlnCys: 0.054 ± 0.01	1.374GlnAsp: 1.374 ± 0.047	2.518GlnGlu: 2.518 ± 0.073	0.524GlnPhe: 0.524 ± 0.03	3.253GlnGly: 3.253 ± 0.086	0.321GlnHis: 0.321 ± 0.022	0.744GlnIle: 0.744 ± 0.037	0.95GlnLys: 0.95 ± 0.043	1.476GlnLeu: 1.476 ± 0.046	0.398GlnMet: 0.398 ± 0.025	0.639GlnAsn: 0.639 ± 0.035	0.783GlnPro: 0.783 ± 0.041	0.452GlnGln: 0.452 ± 0.037	1.726GlnArg: 1.726 ± 0.052	0.911GlnSer: 0.911 ± 0.037	0.88GlnThr: 0.88 ± 0.041	2.648GlnVal: 2.648 ± 0.073	0.2GlnTrp: 0.2 ± 0.019	0.447GlnTyr: 0.447 ± 0.029	0.0GlnXaa: 0.0 ± 0.0
Arg	9.481ArgAla: 9.481 ± 0.137	0.274ArgCys: 0.274 ± 0.023	2.631ArgAsp: 2.631 ± 0.062	7.763ArgGlu: 7.763 ± 0.128	3.919ArgPhe: 3.919 ± 0.073	6.594ArgGly: 6.594 ± 0.109	1.652ArgHis: 1.652 ± 0.052	2.878ArgIle: 2.878 ± 0.072	3.048ArgLys: 3.048 ± 0.076	13.041ArgLeu: 13.041 ± 0.186	1.63ArgMet: 1.63 ± 0.048	1.149ArgAsn: 1.149 ± 0.045	5.261ArgPro: 5.261 ± 0.103	1.791ArgGln: 1.791 ± 0.064	7.634ArgArg: 7.634 ± 0.128	2.205ArgSer: 2.205 ± 0.062	2.914ArgThr: 2.914 ± 0.071	7.767ArgVal: 7.767 ± 0.109	0.961ArgTrp: 0.961 ± 0.045	3.045ArgTyr: 3.045 ± 0.068	0.0ArgXaa: 0.0 ± 0.0
Ser	3.238SerAla: 3.238 ± 0.076	0.188SerCys: 0.188 ± 0.016	1.057SerAsp: 1.057 ± 0.042	2.014SerGlu: 2.014 ± 0.058	1.529SerPhe: 1.529 ± 0.049	3.765SerGly: 3.765 ± 0.098	0.622SerHis: 0.622 ± 0.029	0.83SerIle: 0.83 ± 0.04	0.803SerLys: 0.803 ± 0.042	5.57SerLeu: 5.57 ± 0.107	0.512SerMet: 0.512 ± 0.029	0.431SerAsn: 0.431 ± 0.026	3.366SerPro: 3.366 ± 0.083	1.008SerGln: 1.008 ± 0.039	2.857SerArg: 2.857 ± 0.072	1.235SerSer: 1.235 ± 0.063	1.42SerThr: 1.42 ± 0.053	2.416SerVal: 2.416 ± 0.063	0.566SerTrp: 0.566 ± 0.033	0.976SerTyr: 0.976 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	3.539ThrAla: 3.539 ± 0.089	0.208ThrCys: 0.208 ± 0.018	1.369ThrAsp: 1.369 ± 0.051	2.393ThrGlu: 2.393 ± 0.062	1.45ThrPhe: 1.45 ± 0.043	4.113ThrGly: 4.113 ± 0.082	0.825ThrHis: 0.825 ± 0.033	0.919ThrIle: 0.919 ± 0.051	0.901ThrLys: 0.901 ± 0.041	6.085ThrLeu: 6.085 ± 0.097	0.434ThrMet: 0.434 ± 0.025	0.59ThrAsn: 0.59 ± 0.035	3.675ThrPro: 3.675 ± 0.084	0.863ThrGln: 0.863 ± 0.034	2.606ThrArg: 2.606 ± 0.059	1.453ThrSer: 1.453 ± 0.049	1.712ThrThr: 1.712 ± 0.072	2.636ThrVal: 2.636 ± 0.068	0.556ThrTrp: 0.556 ± 0.036	1.197ThrTyr: 1.197 ± 0.05	0.0ThrXaa: 0.0 ± 0.0
Val	9.174ValAla: 9.174 ± 0.117	0.324ValCys: 0.324 ± 0.024	3.389ValAsp: 3.389 ± 0.073	7.291ValGlu: 7.291 ± 0.121	2.961ValPhe: 2.961 ± 0.073	6.901ValGly: 6.901 ± 0.111	1.581ValHis: 1.581 ± 0.058	2.029ValIle: 2.029 ± 0.073	2.407ValLys: 2.407 ± 0.069	11.362ValLeu: 11.362 ± 0.147	1.209ValMet: 1.209 ± 0.047	1.514ValAsn: 1.514 ± 0.046	4.585ValPro: 4.585 ± 0.077	2.023ValGln: 2.023 ± 0.059	7.339ValArg: 7.339 ± 0.102	2.711ValSer: 2.711 ± 0.069	2.466ValThr: 2.466 ± 0.073	7.52ValVal: 7.52 ± 0.121	0.964ValTrp: 0.964 ± 0.043	2.768ValTyr: 2.768 ± 0.069	0.0ValXaa: 0.0 ± 0.0
Trp	1.642TrpAla: 1.642 ± 0.055	0.051TrpCys: 0.051 ± 0.009	0.581TrpAsp: 0.581 ± 0.031	1.19TrpGlu: 1.19 ± 0.043	0.363TrpPhe: 0.363 ± 0.024	1.283TrpGly: 1.283 ± 0.049	0.25TrpHis: 0.25 ± 0.021	0.367TrpIle: 0.367 ± 0.024	0.428TrpLys: 0.428 ± 0.027	1.559TrpLeu: 1.559 ± 0.051	0.282TrpMet: 0.282 ± 0.02	0.285TrpAsn: 0.285 ± 0.024	0.92TrpPro: 0.92 ± 0.038	0.428TrpGln: 0.428 ± 0.028	1.346TrpArg: 1.346 ± 0.045	0.491TrpSer: 0.491 ± 0.028	0.66TrpThr: 0.66 ± 0.032	1.152TrpVal: 1.152 ± 0.044	0.209TrpTrp: 0.209 ± 0.018	0.318TrpTyr: 0.318 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.53TyrAla: 3.53 ± 0.076	0.133TyrCys: 0.133 ± 0.014	1.065TyrAsp: 1.065 ± 0.041	1.77TyrGlu: 1.77 ± 0.053	0.988TyrPhe: 0.988 ± 0.047	2.7TyrGly: 2.7 ± 0.059	0.592TyrHis: 0.592 ± 0.034	0.618TyrIle: 0.618 ± 0.031	0.652TyrLys: 0.652 ± 0.035	4.527TyrLeu: 4.527 ± 0.087	0.318TyrMet: 0.318 ± 0.02	0.417TyrAsn: 0.417 ± 0.031	2.066TyrPro: 2.066 ± 0.055	0.842TyrGln: 0.842 ± 0.034	3.075TyrArg: 3.075 ± 0.076	0.84TyrSer: 0.84 ± 0.04	1.477TyrThr: 1.477 ± 0.051	1.892TyrVal: 1.892 ± 0.055	0.437TyrTrp: 0.437 ± 0.027	0.815TyrTyr: 0.815 ± 0.039	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.002XaaLeu: 0.002 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.002XaaVal: 0.002 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2227 proteins (663962 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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