Prochlorococcus phage P-SSM7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Palaemonvirus; Prochlorococcus virus PSSM7

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 237 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3SNG0|E3SNG0_9CAUD Uncharacterized protein OS=Prochlorococcus phage P-SSM7 OX=445688 GN=PSSM7_042 PE=4 SV=1
MM1 pKa = 7.23SQLNVDD7 pKa = 4.26ALKK10 pKa = 10.55HH11 pKa = 5.09SQGTGPGIDD20 pKa = 3.89LQSSGNFAFDD30 pKa = 3.5TNTLYY35 pKa = 11.02VDD37 pKa = 3.85SVNDD41 pKa = 3.45RR42 pKa = 11.84VGINDD47 pKa = 3.51ATPSHH52 pKa = 6.49SLDD55 pKa = 3.36VAGSDD60 pKa = 4.68GVNLGCGAIFEE71 pKa = 4.68KK72 pKa = 11.19VNIVSGTSNGNTNIDD87 pKa = 3.94LLTSSVHH94 pKa = 6.74LFTSNNTGNWTPNFRR109 pKa = 11.84GDD111 pKa = 3.37ASTSLDD117 pKa = 3.61SIMSTGQVIVATIISDD133 pKa = 3.51NGGSSGYY140 pKa = 9.21AANMNIDD147 pKa = 3.16GSGQTEE153 pKa = 4.56YY154 pKa = 10.21WSNDD158 pKa = 2.68ATPDD162 pKa = 3.78DD163 pKa = 4.18RR164 pKa = 11.84GGTSGYY170 pKa = 10.73DD171 pKa = 3.15VYY173 pKa = 10.98QYY175 pKa = 11.48SIIKK179 pKa = 8.48TGAGNSYY186 pKa = 10.64LVLANRR192 pKa = 11.84TYY194 pKa = 10.78MDD196 pKa = 3.19

Molecular weight: 20.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3SNE9|E3SNE9_9CAUD Uncharacterized protein OS=Prochlorococcus phage P-SSM7 OX=445688 GN=PSSM7_031 PE=4 SV=1
MM1 pKa = 7.39IFWIGFTLMFFNEE14 pKa = 4.41GFVMMRR20 pKa = 11.84HH21 pKa = 4.9VSPWFSNQRR30 pKa = 11.84QKK32 pKa = 11.21FIDD35 pKa = 3.74RR36 pKa = 11.84YY37 pKa = 9.64GANVWYY43 pKa = 9.96RR44 pKa = 11.84FHH46 pKa = 6.83GTLDD50 pKa = 3.63YY51 pKa = 10.67TWIGLVSIGLIVNPNRR67 pKa = 11.84ILHH70 pKa = 6.15IAALATFWSLSFIIFYY86 pKa = 10.09LPRR89 pKa = 11.84WIKK92 pKa = 10.46RR93 pKa = 3.41

Molecular weight: 11.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

237

0

237

58727

25

7124

247.8

27.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.763 ± 0.25

0.879 ± 0.102

6.765 ± 0.146

5.863 ± 0.329

4.185 ± 0.082

7.892 ± 0.347

1.568 ± 0.118

6.547 ± 0.164

6.156 ± 0.476

7.106 ± 0.176

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.975 ± 0.218

5.849 ± 0.164

3.809 ± 0.136

3.458 ± 0.123

3.847 ± 0.202

7.351 ± 0.274

8.03 ± 0.577

6.76 ± 0.201

1.136 ± 0.085

4.059 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski