Scytonema millei VB511283

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Scytonemataceae; Scytonema; Scytonema millei

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4950 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D2PE31|A0A1D2PE31_9CYAN FAD-dependent oxidoreductase OS=Scytonema millei VB511283 OX=1245923 GN=QH73_021535 PE=4 SV=1
MM1 pKa = 7.31TRR3 pKa = 11.84FDD5 pKa = 4.36GDD7 pKa = 4.14SNNNTLNGTAGDD19 pKa = 4.14DD20 pKa = 3.51EE21 pKa = 5.66LYY23 pKa = 10.79GYY25 pKa = 10.31DD26 pKa = 4.26GNDD29 pKa = 2.89ILFGGDD35 pKa = 3.78GNDD38 pKa = 3.32TDD40 pKa = 4.66KK41 pKa = 11.5LVGGSGDD48 pKa = 4.54DD49 pKa = 3.48ILIGSIGKK57 pKa = 9.44DD58 pKa = 2.83LFIFNDD64 pKa = 3.23YY65 pKa = 10.08EE66 pKa = 4.83YY67 pKa = 11.18NVSIYY72 pKa = 10.65KK73 pKa = 10.08DD74 pKa = 3.16AYY76 pKa = 9.39NNYY79 pKa = 9.65HH80 pKa = 5.54VFSSEE85 pKa = 3.49GHH87 pKa = 6.75DD88 pKa = 3.32IITNFSEE95 pKa = 4.61AEE97 pKa = 3.63GDD99 pKa = 3.6KK100 pKa = 10.61IYY102 pKa = 11.31VLDD105 pKa = 4.74PQPADD110 pKa = 4.0PINRR114 pKa = 11.84IKK116 pKa = 10.72PIDD119 pKa = 4.3PIIYY123 pKa = 9.82RR124 pKa = 11.84GSADD128 pKa = 3.75NIKK131 pKa = 9.73TILGANTSQYY141 pKa = 9.88TLSNSSLMLEE151 pKa = 4.36SNILQNPLYY160 pKa = 10.93NNLSNLGADD169 pKa = 3.37TSGFGLL175 pKa = 4.02

Molecular weight: 19.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D2P4A1|A0A1D2P4A1_9CYAN Transmembrane sensor domain-containing protein (Fragment) OS=Scytonema millei VB511283 OX=1245923 GN=QH73_015475 PE=4 SV=1
MM1 pKa = 7.34RR2 pKa = 11.84TFIIIWIGQLVSLLGSGLTGFALSIWVYY30 pKa = 11.23KK31 pKa = 8.84NTNSVTQFALISVLTMLPNILISPLAGGLGDD62 pKa = 3.28RR63 pKa = 11.84RR64 pKa = 11.84NRR66 pKa = 11.84RR67 pKa = 11.84KK68 pKa = 10.35LIILSNCGSGLSILSLALLLFIGHH92 pKa = 6.51LEE94 pKa = 3.42IWHH97 pKa = 6.66IYY99 pKa = 8.73IATAASSAFHH109 pKa = 6.97ALQGPAYY116 pKa = 9.88DD117 pKa = 4.33AVITQLIPPQHH128 pKa = 6.37LVRR131 pKa = 11.84ANGMFQLGLAVAQLISPVLGGILLVTIQIQGIFILDD167 pKa = 4.86GITFIFCLVMLLLVRR182 pKa = 11.84LPKK185 pKa = 10.45PITTTKK191 pKa = 11.11AEE193 pKa = 4.21VPKK196 pKa = 10.86NLLWHH201 pKa = 6.58DD202 pKa = 3.97TLVGWNYY209 pKa = 8.02ITTRR213 pKa = 11.84RR214 pKa = 11.84GLFWLLILNCVYY226 pKa = 10.77HH227 pKa = 6.09FVEE230 pKa = 5.86GIAVVLSTPLFLSFTSTAVLGTMLSIGGSGMILGTALISTWGGAKK275 pKa = 9.46RR276 pKa = 11.84RR277 pKa = 11.84IYY279 pKa = 10.96NVFGFMLLGGLCMLVAGLRR298 pKa = 11.84RR299 pKa = 11.84NVLLCTVAIFLHH311 pKa = 6.53FFSLPIVVSSRR322 pKa = 11.84QAIFQSKK329 pKa = 8.76IAADD333 pKa = 3.24VRR335 pKa = 11.84ARR337 pKa = 11.84VFAVNRR343 pKa = 11.84MLINLSLLLAYY354 pKa = 10.31LVAGPLADD362 pKa = 4.33RR363 pKa = 11.84VFEE366 pKa = 4.22PLLTINGPLAGSVGKK381 pKa = 10.36IIGVGPGRR389 pKa = 11.84GIGLLFIIMGMSLIVAIVTSYY410 pKa = 11.18LSPRR414 pKa = 11.84LRR416 pKa = 11.84LVEE419 pKa = 4.25SEE421 pKa = 4.2LPNVISHH428 pKa = 7.04

Molecular weight: 46.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4950

0

4950

1457382

30

2762

294.4

32.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.892 ± 0.04

1.035 ± 0.012

4.73 ± 0.027

6.347 ± 0.038

4.085 ± 0.023

6.46 ± 0.037

1.89 ± 0.018

6.532 ± 0.026

5.191 ± 0.031

10.94 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.817 ± 0.014

4.412 ± 0.028

4.588 ± 0.024

5.413 ± 0.032

5.185 ± 0.029

6.458 ± 0.031

5.625 ± 0.025

6.838 ± 0.027

1.428 ± 0.016

3.132 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski